I’ve obtained the concentration of a DNA scaffold (ssDNA) in ng/μl. How would I then go about calculating this in μM?
E.g. Conc = 1284.8 ng/μl Mw = 12509.1 g/mole EC = 372300 M-1cm-1 Length = 41
I was bored in the middle of the quarantine and decide to compete against a friend on who can improvise a mask faster. (15 min max).
MASK: (Yes is ugly)
https://imgur.com/a/uWMF8Ad
Mats
- Vacuum bag HEPA certified E11 or superior
- Wire
- 2x rubber bands
- Hot glue gun
- Scissors
Instructions
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Cut the mask shape: https://imgur.com/a/Rezyppu
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Cut the rubber bands https://imgur.com/a/4NyJQin
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Attach rubber bands with Hot glue gun: https://imgur.com/qOi04mZ
(Improvement: make a knot at the end of each side, to increase adherence)
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Attach a Wire with V shape to the mask https://imgur.com/a/C1GCMG1
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Use the mask: https://imgur.com/a/uWMF8Ad
References:
vacuum filter HEPA certified
HEPA class Retention (total)
E10 > 85% -
E11 > 95% - n95 ffp2
E12 > 99.5% -
H13 > 99.95% - n100 ffp3
https://en.wikipedia.org/wiki/HEPA
Misc Tests
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Thermic sealing Could be binded with heat. (180 degrees is too much for the material) https://imgur.com/a/LCvnBCf
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Staples + thermic sealing
https://imgur.com/a/rnlJOlP
- Hut Glue gun sealing (Without Glue)
https://imgur.com/a/Ay561Wm
( my friend test)
Journal of the American Chemical SocietyDOI: 10.1021/jacs.0c05392
Kentaro Tomita, Hidenori Noguchi, and Kohei Uosaki
https://ift.tt/31qxXjG
Nanomotors: The first example of a DNA origami nanomotor that processively converts chemical fuel into sustained mechanical motion is presented. Tunable DNA origami motors travel linearly for micron distances at nm/s speeds.
Abstract
Inspired by biological motor proteins, that efficiently convert chemical fuel to unidirectional motion, there has been considerable interest in developing synthetic analogues. Among the synthetic motors created thus far, DNA motors that undertake discrete steps on RNA tracks have shown the greatest promise. Nonetheless, DNA nanomotors lack intrinsic directionality, are low speed and take a limited number of steps prior to stalling or dissociation. Herein, we report the first example of a highly tunable DNA origami motor that moves linearly over micron distances at an average speed of 40 nm/min. Importantly, nanomotors move unidirectionally without intervention through an external force field or a patterned track. Because DNA origami enables precise testing of nanoscale structure‐function relationships, we were able to experimentally study the role of motor shape, chassis flexibility, leg distribution, and total number of legs in tuning performance. An anisotropic rigid chassis coupled with a high density of legs maximizes nanomotor speed and endurance.
https://ift.tt/2Or4pMi
This is the best tl;dr I could make, original reduced by 97%. (I'm a bot)
> The MP concentration of Arctic snow was significantly lower than European snow but still substantial.
> DISCUSSIONThis study provides the first data on contamination of snow by MPs. MP concentrations in snow were very high, indicating significant contamination of the atmosphere.
> Some variability may be due to the fact that snow from the Arctic was not freshly deposited unlike all European snow samples and could have been exposed to secondary dry deposition of airborne particles for an unknown period of time.
> Still, the lower concentrations found in Arctic snow is not unusual given the distance of the Arctic to densely populated source areas.
> Abundance of fibersAll snow samples contained fibers ranging between 0.043 103 and 10.2 103 N liter−1 with highest concentrations in snow from Ice Floe 4 followed by snow from Bremen.
> The high MP concentrations detected in snow samples from continental Europe to the Arctic indicate significant air pollution and stress the urgent need for research on human and animal health effects focusing on airborne MPs.The high amount of MPs present in the atmosphere as indicated by significant concentrations in snow from continental Europe to the Arctic is puzzling at first.
Summary Source | FAQ | Feedback | Top keywords: snow^#1 sample^#2 MP^#3 Arctic^#4 particle^#5
Post found in /r/science, /r/EverythingScience, /r/interestingasfuck and /r/science.
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