They were doing free drug analysis in my city today. Dope came back as 82% diacetylmorphine and heroin base and 18% caffeine and acetaminophen. Does anyone know if this is what you would expect from your heroin #3? No fentanyl or other additives detected
I want to get a mass spectrometer machine for the SSP I work at.
I know there are some that are more inexpensive and portable, some that may or may not be able to test many analogs or have a large drug library, etc.
Are there any, when taking capabilities and pricing into account, that anyone here would recommend?
Context: 20yo male, 165lb
4:03 insufflated ketamine 100mg pure
4:15 have achieved god consciousness and turned my phone on silent
4:19 there’s no such thing as happiness and no such thing as sadness. Reality is too complex to understand
4:22 consciousness is too profound to be described by a single word like happy.
4:23 but I guess that could describe it
4:32: only I am real. Everyone else is a story to get me to behave in a certain way.
4:32: but Buddhism has taught me that I don’t exist. Then what is real?
4:35 suffering can’t be quantified because
4:39 once we believed in gods; now we are gods
4:45: hold up. What if the forces of the universe are essentially good?
4:46: even if our reality is simulated I hope it’s for a good cause
4:47 I should post this on Reddit
4:48 this is some Flowers for Algernon shit
Hello, I am new to mass spectrometry. My background was molecular biology with some specialization in bioinformatics. I will be joining a research laboratory that specializes in nanoLC-MS/MS + proteomics for my Master's. I am asking the questions below so I can make a better research proposal and a more informed choice in the future.
For my Master's, I want to do two things (these don't necessarily have to be congruent with each other). One, I want to make a differential proteomics analysis between a mammalian cell line and a biopsy tissue sample. Two, I want to do a cancer biomarkers mining analysis using LC-MS/MS as a potential pipeline/proof-of-concept for MS-based cancer clinical diagnostics.
On the other hand, the closest relevant publication (w/ respect to my research goal) that my lab has done did a comparative proteomic analysis in a bacteria. They used 2 MS instruments. One is the Q-Exactive mass spectrometer (which I read is an Orbitrap-based MS), and the other is a TripleTOF 5600+ mass spectrometer (which I read is a Triple Quadrupole/TOF-based MS). For their LC, they used a Dionex Ultimate 3000 RSLCnano system coupled with an HTC-PAL autosampler.... and then after that, based on the semiquantitative proteomic profiling data based on the number of identified MS/MS spectra per protein, they used a custom NR database to do cluster analysis, proteome profiling, and they compare hit counts of differentially expressed proteins.
My general question is: Do you think my intended research goal and the lab that I will be joining are compatible? Do you think the lab that I will be joining has enough proficiency to help guide me to do my intended project?
Furthermore, here are some specific/technical questions that I have:
- How different will bacterial-proteomics be from mammalian-based proteomics in terms of sample prep?
- Additionally, correct me if I'm wrong, but I assume after the sample-prep and the LC-MS/MS run, the data analysis between the bacterial-proteomics and the mammalian-based proteomics is just the same, right? The only thing that will change is the NR database that I will use?
- Between the 2 MS instruments (the QE-MS orbitrap and the TripleTOF), is one better than the other in terms of its compatibility with my intended project?
- Lastly, the lab just did a comparison of keyword hit counts of differentially expressed proteins, but they didn't really identify specific proteins that are upregulated or downregul
It just occurred to me that Aperture (may have?) had successful quantum tunneling tech in the 50's and Black Mesa had miniaturized Xen portals by the 90's at the latest, implying that they'd been working on the project for quite some time.
So what was the point of the Anomalous Materials team?
The race for portal technology is already over and has been potentially for decades.
Both companies already created portals so whatever Gordon's team was supposed to be working on is doubly redundant.
I've been thiking about this recently1
https://preview.redd.it/4mduyhxxh4f61.png?width=1920&format=png&auto=webp&s=85ed71ae15ff0477d20f60e1b967590c1227da62
Hi everybody,
I'm having issues tuning a Thermo ISQ 7000 single quadrupole mass spectrometer. I've tried cleaning the ion source multiple times, changing the column, and replenishing the calibration standard reservoir. Each time, tuning fails because the detector cannot find the initial peak. The instrument is fairly new and seldom used. If anybody has input, I would greatly appreciate it.
Thanks!
CW
So, in "Death From Above" Bishop and Palmer accidently blow up Abby's mass spectrometer when trying to esacape, and are terrified she will find out, and at the end of the following episode she seems to be on the verge of doing so. Then the whole thing is seemingly forgotten about and she leaves in "Two Steps Back", seemingly without ever finding out about it. So, why was this whole plot point just dropped for now apparent reason?
So, being an actual designer of actual sample insertion systems into ACTUAL mass spectrometers (but not anti-mass 😉)
I think the entire Black Mesa incident could have been avoided by automating the process..... Did they really need a HUMAN to
- push a button
- push a cart
Like, linear actuator maybe?
Sure, UHV presents an issue for material compatibility but.... Oh, wait...... What vacuum? That sort of kit needs a vacuum. Poor scientists in the airlock would have gone 🤯
Other than that. All other science is sound 👍
theres also the scientist voice lines relating to the incident that can be heard
Context: 20yo male, 165lb
4:03 insufflated ketamine 100mg pure
4:15 have achieved god consciousness and turned my phone on silent
4:19 there’s no such thing as happiness and no such thing as sadness. Reality is too complex to understand
4:22 consciousness is too profound to be described by a single word like happy.
4:23 but I guess that could describe it
4:32: only I am real. Everyone else is a story to get me to behave in a certain way.
4:32: but Buddhism has taught me that I don’t exist. Then what is real?
4:35 suffering can’t be quantified because
4:39 once we believed in gods; now we are gods
4:45: hold up. What if the forces of the universe are essentially good?
4:46: even if our reality is simulated I hope it’s for a good cause
4:47 I should post this on Reddit
4:48 this is some Flowers for Algernon shit
