I am not saying this without any basis. This virus was manufactured in China. It hit Trump, China's #1 enemy and it will probably also take Modi down.
'Far more likely' coronavirus came from lab, ex-MI6 chief tells LBC | Coronavirus was more likely to have escaped from a lab than to have come from an animal, the former head of MI6 has told LBC
https://www.lbc.co.uk/news/coronavirus-escaped-from-lab-mi6-chief/
Dr. Sanjay Gupta on Why He Gives Weight to ‘Informed’ Theory That Covid Leaked From Wuhan Lab https://www.mediaite.com/podcasts/the-interview-dr-sanjay-gupta-talks-vaccines-and-why-he-gives-weight-to-informed-theory-that-covid-leaked-from-wuhan-lab/
Ex-CDC director says he believes coronavirus originated in Wuhan lab
https://www.axios.com/wuhan-lab-coronavirus-cdc-director-c599cf7b-9e30-4314-909b-a9bdac28ead6.html
https://np.reddit.com/r/China_Flu/comments/fbt49e/the_who_sent_25_international_experts_to_china/
Public health agency ordered to hand over documents on two scientists fired from microbiology lab | Xiangguo Qiu sent a shipment of Ebola and Henipah viruses to the Wuhan Institute of Virology in 2019 but PHAC said that had nothing to do with her subsequent exit
https://ottawacitizen.com/news/canada/phac-ordered-to-hand-over-documents-on-two-fired-scientists
Specifically, do you guys just search for rare dens and keep doing those until eventually you get the monster you want with good genes and open slots, or do you paintball your target monster and only do dens that have their eggs.
Hello all,
I hope this is the right subreddit for this question. I would like some input from people with experience in gene editing and various positive and negative selection markers. I have previously used neomycin resistance (neoR) for positive selection and diphtheria toxin (DTA) for negative selection against random integrations.
Positive selection usually works quite well in that all clones contain neoR but negative selection does not select so well against random integration. By Southern blot, 90-95% of clones would still be random integrations (I have previously targeted two loci by traditional gene targeting).
Is there a better negative selection marker (like thymidine kinase [TK])? Would a dual DTA and TK strategy work better? How about putting a DTA cassette at the 5' and 3' ends of the linearized targeting vector?
On paper, it seems like negative selection should kill all random integrations, but decades of papers have shown this not to be the case.
Thank you!
