All I remember is the scene were a person is going though an ink blot test not reacting to any of them then getting to a single dot a freaking out. Something about it reminding them of the constant pressure to be perfect. Idk what time this was from I just remember seeing it as a kid in the 2000s.
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Citation: A Elbrecht, DR Rowley, BW O'Malley - BMBiochemica 4:12-13, 1987
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DOI/PMID/ISBN: unknown
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URL: https://scholar.google.com/scholar?q=Irish+cream+liqueur+as+a+blocking+agent+for+DNA+dot+blots&btnG=&hl=en&as_sdt=0%2C44
This is one of those papers that is mentioned pretty often (hey, let's use our grant money to buy booze) but may not even be real paper. Since it's April 1, I decided to get to the bottom of it. I can't find much about the journal but there is a company with that name so maybe this was some sort of technical bulletin. The last author, at Baylor, does list the paper on his CV. It's been cited 12 times and I can find other papers that use Irish cream for blocking but am interested in the original source. It's not on sci-hub.
Thank you
I'm trying to figure out if my antibody even reacts with my protein by doing dot blots before running any samples on a Western (the way we do them in my lab is a huge pain in the ass). Problem is, we use HRP-conjugated antibodies and ECL substrate for detection, and there's no way to get all the heme from host blood out of my samples. The ECL substrate is reacting with the heme and ruining any chance of detecting the HRP. On a Western, I can just cut off the bottom of the blot where the heme is and process that separately, but that's still not ideal. This is the only method I've ever used for Westerns - is there anything else out there that would be appropriate for my assay?
I don't want to step on anybody's toes here, but the amount of non-dad jokes here in this subreddit really annoys me. First of all, dad jokes CAN be NSFW, it clearly says so in the sub rules. Secondly, it doesn't automatically make it a dad joke if it's from a conversation between you and your child. Most importantly, the jokes that your CHILDREN tell YOU are not dad jokes. The point of a dad joke is that it's so cheesy only a dad who's trying to be funny would make such a joke. That's it. They are stupid plays on words, lame puns and so on. There has to be a clever pun or wordplay for it to be considered a dad joke.
Again, to all the fellow dads, I apologise if I'm sounding too harsh. But I just needed to get it off my chest.
Do your worst!
They were cooked in Greece.
I'm surprised it hasn't decade.
For context I'm a Refuse Driver (Garbage man) & today I was on food waste. After I'd tipped I was checking the wagon for any defects when I spotted a lone pea balanced on the lifts.
I said "hey look, an escaPEA"
No one near me but it didn't half make me laugh for a good hour or so!
Edit: I can't believe how much this has blown up. Thank you everyone I've had a blast reading through the replies 😂
Don't you know a good pun is its own reword?
It really does, I swear!
Because she wanted to see the task manager.
Heard they've been doing some shady business.
but then I remembered it was ground this morning.
Edit: Thank you guys for the awards, they're much nicer than the cardboard sleeve I've been using and reassures me that my jokes aren't stale
Edit 2: I have already been made aware that Men In Black 3 has told a version of this joke before. If the joke is not new to you, please enjoy any of the single origin puns in the comments
What exactly is a culinary ethnography?
Food is many things, to many people. What exactly that thing is, says a lot about that group of people as a whole. What we eat is a huge part of who we are, who we’ve been, and what we experience as we encounter the new. In my 90 years, I have done my best to experience first hand the many, many ways that each denizen of the Forgotten Realms decides to break bread.
This is by no means a rule book, as if I am some sort of dictator of what is, and isn’t proper for a member of a race to do in the kitchen. Rather, it is a collection of observances in regards to commonalities and trends among members of the same race. While my own experience is obviously limited, and there are plenty of individuals I have yet to dine with and learn about, I would like to offer up what I have learned so far.
-Adelbert Boffin, Halfling Culinarian
First things first, as a disclaimer of all Culinary Ethnography entries, let’s set some caveats. Who are Elves? Am I including Half-Elves? Drow? All those of Elven lineage? All those of Elven parentage back to the Fae themselves? While I would like to eventually include more varied looks at the similarities and differences between all of these groups, for the purposes of this analysis, I will be looking at those Elves that live in, or near the Faewild in particular. As always, this is a look at some of the major trends I have seen occur. It is not a rulebook that one must follow to be a “proper Elf” in the kitchen. Food is as much culture as it is history, geography, necessity, community, and personal freedom. With that out of the way, let’s give this culture a respectful look.
Living off the Land
To truly understand the cuisine of the Elves, it is important to first understand where their sustenance is derived from. While this maxim is true of all races, it holds even greater weight with Elves. Food culture is derived from the culmination of necessity and environment and the environment that most Elves live in is one of true bounty, allowing for a more “particular” palate. After all, it is much easier to only eat things that taste good, when starvation is not on the line. Not many races can claim the same abundance, leading to a rather unique culinary culture.
The first subject of note is that Elves do not take part in animal husbandry. From Dwarves, to Humans, to Orcs, you can see some sort of animal that is a mainstay in their diet in some form or another. As always, this is co
... keep reading on reddit ➡They’re on standbi
A play on words.
My daughter, Chewbecca, not so much.
Pilot on me!!
