Vitro Puns

In vitro fertilisation (IVF) is a process of fertilization where an egg is combined with sperm outside the body to assist with the conception of a child.

However, in the process, thousands upon thousands of fertilized embryos are destroyed. If you believe that abortion is murder since you’re knowingly killing a fertilized embryo, then are you also against IVF?

What is NoMeat?

We want to develop an inexpensive way to create artificial meat and bring it to market. The main reason for this project is that we want to stop the slaughter of innocent animals. In addition, by keeping the animals no longer needed, the CO2 pollution is reduced and our world is healthy again. As you can see, we want to help humanity a bit with our project.

Tokenomics:

Total Supply: 100,000,000,000

- 3% Returned to Holders

- 3% Burned

Contract: 0x089e60fd3b652a99781e3c788e98e2e13a28d6ea

🥞 Pancakeswap: https://exchange.pancakeswap.finance/#/swap?outputCurrency=0x089e60fd3b652a99781e3c788e98e2e13a28d6ea

50% BURNED 🔥: https://bscscan.com/tx/0x740a26eccbe121b76e2b0823f2bb91d838b9b652783e7b4baf0f71cca8f06d7f

Ownership RENOUNCED 🔥: https://bscscan.com/tx/0xf079d90a039d7ac3771e5bf595daabd5f1a473f425a69aa66529a920c4642d42

Chart:

https:// poocoin.app/tokens/0x089e60fd3b652a99781e3c788e98e2e13a28d6ea

Links:

✅ Website: https://www.nomeat-finance.biz/

✅ Telegram: https://t.me/nomeatcoinfinance

What is cultured meat?

Cultured meat is meat produced by in vitro cell culture of animal cells, instead of from slaughtered animals It is a form of cellular agriculture.

Cultured meat is produced using many of the same tissue engineering techniques traditionally used in regenerative medicine.The concept of cultured meat was popularized by Jason Matheny in the early 2000s after co-authoring a seminal paper on cultured meat production and creating New Harvest, the world's first nonprofit organization dedicated to supporting in vitro meat research.

As always - no financial advice - do your own research - but this is something unique and real good idea!

link

>The licorice root extract exhibited neutralizing effects even at a subtoxic concentration of 2 mg/ml, which is lower than the typical consuming dilution. For example, in tea, it is 12.5 mg/ml. Although this shows that licorice root tea may be a candidate for complementary use as an antiviral, it is crucial that the active compound is identified and characterized for its potential consideration in clinical applications.

“Complete virus neutralization was achieved at subtoxic concentrations of 0.5 mg/ml under pre- and 1 mg/ml under post-entry conditions.”

> *Important Notice bioRxiv publishes preliminary scientific reports that are not peer-reviewed and, therefore, should not be regarded as conclusive, guide clinical practice/health-related behavior, or treated as established information.

garden for free. Tony berating him them handing him the newspaper cover from his driveway as though he is his personal litter collector. Boss move. Made me laugh. 🤣🤣

I was surprised searching this subreddit that there weren't more posts about this. Sorry if this is a little long, but maybe it could help someone who is going through something similar, or in the future, save someone from going through hundreds of dollars of tests before considering a t-break first. Ultimately, I'd just hopefully like to hear a success story or two. It would be a little lame if I do this the wife and I still have to go through in vitro, but I would kick myself for life if I never tried. Being a dad is all about pushing through and trying hard to do what you need to do for your child anyways.

Background: wife and I are in our mid 30s and she does not smoke and rarely drinks. I am a daily bong smoker for 17 years while only taking breaks when traveling. Fortunatley, that used to be for 2-3 weeks at a time 3-4 times a year. This allowed a balance in my body and mind by being forced to take t-breaks, I believe. Now with COVID and no travel, I haven't had a break in a year and a half and I have never gone this long smoking without a break since I started. Mentally, I've had some weird AF days, and I also realize that drinking a tall can of beer or two a day for years is related. COVID shittiness and suddenly I was at 4-6 beers a day, along with 3-6 bong bowls daily. I recently found out that the cans have plastic lining and could be a BPA contributor, also known to affect sperm, so I am not saying that that marijuana is the main source with what is going on with low sperm counts. I am, however, quite healthy in most other facets of my life (rarely fast food, I cook all the time, almost always fresh stuff, I have like 3 cans of soda a year, don't binge drink liquor, but I used to years ago). I started consuming legal weed sodas and edibles two times a week since last summer and I feel it is a factor to a change in my sperm... uh.. I dunno, it just became more watery like last September or October.
My wife and I have been trying to get pregnant for a few years and we have been seeing specialists since January. I have done every test they asked me with 2 sperm sample tests and 2 bouts of bloodwork. At first, I thought there must be a mistake with my sperm count from the first sample test. I thought something was off and decided to cut the drinking and smoking for a few weeks to a couple bowls a day, no edibles and a 2 or 3 beers, along with 3 days a week doing cardio and weights. Then I had another two weeks going back to my s

I know that his income was halved by losing part of his territory, but how much more was he losing by having to landscape Tony and Johnny Sac's properties 'on the comp'?

Basically, the title is what I think is going on in my cells. I have been getting some confusing results so I knocked down the gene I am studying with lentiviral shRNA in a 12-well and harvested 3x wells every 2 days for 8 days. (I have gotten up to 90% KD efficiency with this shRNA within the last 6 months under different conditions.)

At Day 2 there was a ~50% KD efficiency and already some significant differences on the expression of other related genes between SC and KD. At day 4, there was a ~10% KD efficiency and the expression of my gene had increased in both conditions relative to day 2. Additionally, there were even larger, more significant differences in the expression of other related genes between SC and KD. This trend continued through day 8: on Day 8 the expression of my gene (in both SC and KD) was the highest out of any day, KD efficiency was maybe ~5%, and there were HUGE differences in expression of other related genes (like RQ=20 in SC, RQ=150 in KD, p<0.0001). Here is a link to the plots if my description didn't make sense! https://imgur.com/a/6252Rtl

Do you guys think the title is a suitable hypothesis for what is going on here? My PI keeps telling me i need to make better virus or find a way to infect the cells better, or even design new primers against my target gene I am knocking down. To me, however, it feels clear that that my KD is working but that depleting my protein in turn positively regulates its own expression through some downstream mechanism. I think this is especially evidenced by the good (but not great) KD efficiency early on (day 2) after KD, and the huge effects that are seen in the KD group on day 8 despite a low KD efficiency.

Does anyone have any ideas? Thanks for reading!

Oops just realized i confused two of my experiments, meant to say i harvested cells on days 3/5/7. Should've drank my coffee before writing this post

I'm looking to implement a in vitro degradation assay for a gelatin based biomaterial. Has anyone any experience with such a project or familiar with the applicable standards?

Does that mean that mirtazapine inhibits other drugs that use CYP3A4 enzyme to be metabolised?

So that means if there is another drug that is metabolized by cy3pa4 it will produce less desired effects?

I was wondering what the community has to say about this study.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4956236/

"In conclusion, NaCl solution stimulates hGF cell migration, alters the organization of cytoskeletal molecules (FAK and F-actin), and enhances extracellular matrix gene expression (COL1 and Fn). These data provide the first scientific evidence to support the application of salt solution as mouth-rinse in conjunction with routine oral care to promote oral wound healing."

So I stumbled upon this article randomly while googling. Have any of you read it before and what do you guys think about it?

Here is the article: "TESTING STANDARDS AND LABELING OF UVA PROTECTION"

Most of this science mumbo jumbo goes right over my head, but I saw people reacting strongly/having intense discussions about sunscreen following the third-party testing results like here, here, and here.

The takeaway for me:

- I still don't understand the British Boots system lmao

- PPD and European UVA-PF are, actually, not the same thing

- In vitro means cells on a plate (calculated to be as close to human skin as possible? MATH) and in vivo means tested on human beans

- American law was written by dinosaurs

- "The in vitro UVA-PF method is an equivalent alternative to the in vivo PPD method... In practice, the UVA-PF numbers obtained by one method or the other are very similar. "

What does this mean? They're supposed to result in very similar numbers? I saw a lot of people/companies saying the third party test results are garbage because the testing methods were different.

I am confusion because I see that Bioderma and Nivea sunscreens pretty much always meet their claims no matter where and how they were tested, it looks like. I get testing sunscreen is really hard, formulating is really hard, but my brain is like, "but Bioderma and Nivea done it.."

- I always thought antioxidants increase sun protection in sunscreens but according to this, they don't.

Food for thought. :>

What is my obligation to speak up about the immorality of something when I have a brief meeting with someone outside of my circle and they are talking about engaging in an immoral activity?

I went to get my haircut last week and the hairdresser started talking about how she was getting eggs implanted or something like that, the next day.

I didn’t encourage it or praise it in any way. In fact, I told a story about my cousin who had 12 years of infertility and was able to conceive spontaneously after she received a relic. I spoke about being Catholic positively and about miracles and how happy they were.

The lady then said something about well if it’s quadruplets, she didn’t know, the doctor didn’t want that many; he only wanted one. So she would see what he would do. At the time I didn’t really think fully about reduction/destruction of embryos, but later I thought that might have been her reference.

I didn’t say in vitro was immoral because I didn’t know what good it could do. I guess I could have said it was against my religion, I don’t know.

Just wondering if I have committed a sin of omission here. I did pray some Hail Marys for her as I sat there. I prayed the doctor would be unsuccessful. I just feel like I was in a trap and there was seemingly no good way out.

Advice about sin here and about what I could do in the future.

Thanks 🙏🏻

Just been very paranoid lately by all these sunscreen scandals, and I was curious as to which method of testing is more reliable when it comes to determining the protective factors of sunscreens when it comes to UVA and UVB protection. Sorry if this is a crazy over the top question but I just wanna be sure I’m getting the best possible protection I could get!

This research is the first to investigate the anti-skin wrinkle properties of Cordyceps militaris extracts both in vitro and in vivo .

Anti-skin wrinkle activities of C. militaris were investigated by means of matrix metalloproteinase-1 (MMP-1), elastase, and hyaluronidase inhibitions. Microemulsions and topical serum formulation containing C. militaris extract were developed.

The anti-skin wrinkle efficacy and irritation properties of the topical serum formulations were clinically investigated in human volunteers. Cordycepin was identified as a major component of C. miliaris extract that was responsible for MMP-1, elastase, and hyaluronidase inhibition.

The C. miliaris water extract possessed the most potent inhibition on MMP-1 (77.9 ± 5.3%) and elastase (84.4 ± 4.0%). Interestingly, CW was as a potent MMP-1 and elastase inhibitor as oleanolic acid and EGCG. CW was incorporated into the microemulsion with the smallest internal droplet size (146.1 ± 1.5 nm) and further developed as a topical serum formulation.

The resulting serum formulation effectively enhanced skin moisture (42.2 ± 14.2%), increased the skin elasticity (39.9 ± 7.3%), and induced no skin irritation in 30 human volunteers. The effectiveness on the skin was detected after 1 week of the applications.

Therefore, it was suggested as an effective anti-skin wrinkle formulation.

From :

- https://europepmc.org/article/ppr/ppr354132

As I understand, the sperm is chosen under the microscope by a person, based on the motility, look. I am interested if there are any differences between group of people, that were IVF and the control group? Those can be any kind of differences, like athleticism, intelligence, disease prevalence, mental health. I would be also very thankful for links to the studies.