https://preview.redd.it/t0teqwnktw981.jpg?width=595&format=pjpg&auto=webp&s=45ebd89056993f634f17eb9a18dc2b476eef4fe7
Maximum Mutagenesis is now available in PDF and Print on Demand hard cover format.
An Umerican Mutation Sourcebook
May the ever present holy glow warm your marrow as you travel down the irradiated paths of Umerica!
Within these twisted pages you will find:
- Getting the Glow - Looking to get mutated? Here is a list of what you need and where you might find it.
- Lesser Mutations - A new form of simple mutations to inflict upon hapless wastelanders!
- Three new character classes - Nukaβs Favored, Glow Shepherds, and Tainted Drifter.
- The Mutation Alphabet - 26 illuminated tables of new mutagenic mischief.
- Grokkβs Blessed Bestiary - Over two dozen βordinaryβ creatures subjected to the mutagenic effects of the Mutation Alphabet, creating a slew of new horrors for wasteland adventurers to encounter.
This product is compatible with the Dungeon Crawl Classics Role Playing Game
Get it now at...
DrivethruRPG: https://www.drivethrurpg.com/product/378925/Maximum-Mutagenesis-DCC
Amazon: Coming Soon
Goodman Games webstore: Coming Soon
Hi,
I have a quick question. I have an N-terminal tag that I would like to remove from my sequence in the vector. I naturally imagined that all I need to do is mutate a premature stop codon, followed by a start codon before the part of the sequence that I want to express. I do not think this should be a problem because the sequence is still downstream from the promoter, but I feel like that I might be missing some insight. Would it be better to have a spacer of codons separating the premature stop codon and where I want to start the sequence after it? Does this question make sense? I have a depiction below if that helps:
Original:
T7 Promoter------Start Codon------N-terminal tag-----Primary Sequence of Interest------Stop Codon
Proposal:
T7 Promoter--------Start Codon------N-terminal tag------Premature Stop Codon-------New Start Codon---------Primary Sequence of Interest------Stop Codon
I am asking this because it makes more sense to edit the tag away from the sequence than to design a new vector/plasmid. I am only interested in purifying the sequence of interest, so it does not bother me if a small peptide fragment of the previously mentioned tag is expressed. Thanks for your help.
I'm doing a university project on the 1978 Hutchison paper about Site Directed Mutagenesis and it's a particularly difficult paper for me to digest. Virtually, the phi X174 bacteriophage genome is edited enzymatically to have a specific desired mutation (changing G with A at position 587). E. coli would then be transfected with this new phage gene to cultivate mutated phages. This knowledge is pretty bare bones compared what I need to know, and I'm not even entirely sure the steps I stated were correct. Is anyone able to give me some tips?
Hi everyone,
I was trying to perform a site-saturation mutagenesis via PCR. I tried several primer designs and protocols but never have success after transformation. Only a handful of colonies are obtained but would rather aim for at least 100-200.
How are your experiences with this procedure? Can you recommend a robust/reliable single-SSM protocol using PCR and vector template?
We work in E.coli btw.
I would like to try to induce genetic mutations in pepper plants to see the results and create something unique. Is it possible to try mutagenesis at home having no specialized tools? If so, how would I go about it? Could these mutant traits be passed down and become stable? Thank you for your time π
I've had this thought for a while but why is it that no one is talking the risk of getting cancer as a result from the DNA based vaccines like JNJ, Novavax, etc? What's the rationale for the safety of JNJ's vaccine?
Gene therapy does carry risk of insertional mutagenesis and secondary malignancies. This has been seen in human and in vivo. CAR-T therapies are 1 example for secondary malignancies. The FDA even required follow-up studies in their approval letters for CAR-T for this reason. And CAR-Ts use viral vectors as well to deliver the gene. And the rates of secondary cancers are not exactly low...granted different patient population, prior tx, etc. But if 1/1000 get cancer from JNJ vaccine, is that an acceptable risk for people?
So why is no one talking about this risk? I saw people talk in Science but nothing conclusive. I can imagine JNJ will end up with a huge scandal like their recent baby powder and opioid scandals. Bu since vaccine manufacturers are immune to lawsuits now, there will be no recourse for those who get cancer from DNA based viral vector vaccines.
How safe are adenovirus vector's and JNJ's vector compared to lenti or retroviral vectors? I'm hoping there's no risk but papers I read suggest there actually is. I get there's approved vaccines like Ebola but don't you need long-term follow-up on the orders of decades to really see the danger of DNA based vaccination? This seems like a huge gamble to me and why I'm hesitant to use JNJ or any DNA based vaccine.
What are some ways I can improve SDM efficiency? I have ligated the mCherry sequence from pJ014 into pJT0 to make pJT1 (high expression plasmid containing mCherry fluorescent protein). I am now using SDM on pJT1 to convert the plasmid to pJT2 (mOrange). If anyone has past experience on this I would be grateful to know!
https://preview.redd.it/4mr94ysysw981.jpg?width=595&format=pjpg&auto=webp&s=c13bbd105266b845521755fed3eedc8f4ac5f169
Maximum Mutagenesis is now available in PDF and Print on Demand hard cover format.
An Umerican Mutation Sourcebook
May the ever present holy glow warm your marrow as you travel down the irradiated paths of Umerica!
Within these twisted pages you will find:
- Getting the Glow - Looking to get mutated? Here is a list of what you need and where you might find it.
- Lesser Mutations - A new form of simple mutations to inflict upon hapless wastelanders!
- Three new character classes - Nukaβs Favored, Glow Shepherds, and Tainted Drifter.
- The Mutation Alphabet - 26 illuminated tables of new mutagenic mischief.
- Grokkβs Blessed Bestiary - Over two dozen βordinaryβ creatures subjected to the mutagenic effects of the Mutation Alphabet, creating a slew of new horrors for wasteland adventurers to encounter.
This product is compatible with the Dungeon Crawl Classics Role Playing Game
Get it now at...
DrivethruRPG: https://www.drivethrurpg.com/product/378925/Maximum-Mutagenesis-DCC
Amazon: Coming Soon
Goodman Games webstore: Coming Soon
