A list of puns related to "Liquidβliquid extraction"
URL: https://pubs.acs.org/doi/full/10.1021/acs.iecr.7b05221
authors: Zhuo LiZhuo Li,Β Yingna Cui, Yongming Shen and Changping Li
DOI: DOI: 10.1021/acs.iecr.7b05221
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I'm returning to my undergraduate project after a year out from medical absence, so my lab skills are a bit rusty. Part of a synthesis I'm going to be doing involves "solid-partitioning" (a term I've not encountered before) and extracting and washing the mixture:
"The solvent was removed under reduced pressure and the resulting mixture partitioned between EtOAc (50 mL) and H2O (50 mL), the organics were extracted twice more (2 Γ 25 mL), washed with saturated aqueous brine (50 mL), dried over Na2SO4, filtered and the solvent removed under reduced pressure "
How would I "extract" the reaction mixture with water and how is this different to "washing" with brine solution?
Thanks
Hello fellow chromatographers,
I am currently trying to precipitate protein/lipid out from a clear aqueous sample. Traditional methods are acid treatments with perchloric acid, trichloroacetic acid etc.
I am wondering what is the name of this technique called? Is it LLE? But I only have a single phase system, miscible liquids. Is it SLE? Since I am precipitating and discarding the protein (recover aqueous sample). Thank you for answering, baffled analyst here ...
When is a liquid-liquid-extraction needed? In almost any paper I read a liquid-liquid-extraction is performed before flash-chromatography. But wouldn't it be easier to (dry)load the reaction-mixture directly onto the column?
For how long could i store the extracted LSA in water/juice before it goes bad ?
I do an LLE and I want to retain the top phase (which is alcohol). I usually get about 400ul in the top phase, so I can safely pipette off about 350ul.
I need to do this very slowly and carefully, because it is very easy to get some of the bottom phase.
This is fine when I only have a few samples in individual eppendorfs. But really, I need to move towards doing a few hundred at a time- i.e. in a couple 96-well plates.
It's almost impossible to use a manual multi-channel.
My electronic finnpipette is too forceful.
I don't have a robot.
What are my other options here?
i got all 4 wisdom teeth out on august 26th, and on day 9 i noticed some salty-tasting fluid accumulating in my mouth. when i spat in the sink, my spit was clear but slightly yellow-tinged, with some globbier yellow bits mixed in as well.
iβm not visibly swollen, and none of my sockets hurt. i canβt even tell which socket the fluid is coming fromβit pools at the back of my mouth, in the space between my tongue and my two lower extraction sites. just to be safe iβve reverted back to the liquid diet (sigh), rinse all my sockets with a syringe of warm salt water 3 times a day, and brush my teeth twice a day. it hasnβt decreased since it started.
is this an infection, or just βhealing fluidβ? i imagine if it was an infection i would be feeling some amount of pain, but iβm not. and the yellow liquid doesnβt look like pusβitβs a very watery, translucent consistency.
my dentist is closed for the long weekend so i figured i would ask here to see if anyone has had a similar experience.
Just a thought...
I read in several places online of people adding a strong base such as lye or sodium carbonate to liquid methadone to precipitate the freebase, which they extracted in a variety of ways for use in vaporizing. The effects of vaping the freebase were said to be extremely powerful, compared to fentanyl by one and called "the crack of opioids" by another.
As an experiment, I heated about 300mg sodium bicarbonate in a few ml water until bubbling stopped to produce sodium carbonate. I added this to roughly 200mg liquid methadone. A precipitate immediately formed, as evidenced by the clear red liquid turning opaque pink. One of the people who did this online claimed that freezing the suspension would cause the sugars to turn to a goop on the top of the frozen water, which one could carefully remove then evaporate the remaining liquid to achieve smokeable product. I never got any goop, just a milky pink frozen product.
Next I tried thawing it and letting it sit over 24 hours. The precipitate remained suspended. Finally I decided to evaporate the suspension in a large, flat pyrex pan at 250f in the oven until it became a thick syrup, at which time I reduced the temp to 200. I left it at that temp for quite a while, hoping the thick goo would solidify. This never happened.
Next I prepared some anhydrous isopropanol, which I have used to dissolve other freebase piperazine derivatives with ease. I added an excess of sodium chloride to 91% rubbing alcohol, shook well and let it sit. After it settled I decanted off the alcohol. To ensure there was no water in my product, I added a some water to the bottle with the salt in the bottom and shook, then let the layers seperate. Only a very small isopropanol later formed at the top, which told me I used more than enough salt to dehydrate the alcohol the first time.
Finally I poured the anhydrous iso in the pan with the freebase done/additive goop and scraped and stirred well, scooping up the goop that wouldn't dissolve and adding it to a clean bottle into which I decanted the iso from the pan which hopefully contains the freebase. I shook well, let the undissolved crap settle, then decanted the solvent into a glass with a coffee filter rubber banded to the top. I didn't want to clog the filter, so I only poured the clear layer into the filter, but when it's done I intend to filter the remainder.
Right now it's still filtering, but when it's done I intend to evaporate the solution on low in the oven, cracking the door to
... keep reading on reddit β‘#Liquid Extraction (emiter) The user has ability to extract any kind of liquid substances from its source and store it inside his body. He can then inject or secrete the substance from their body. He then also has ability to control and manipulate those substances, even able to combine them or mix them to make new substance. Each substance will connected to his finger. Once he extract a substance, one of his finger will turn black, it also indicates the % amount of the substance.
Drawback: The user must know what substance he wants to extract from its source. He is limited to store maximum of 10 different substances. To much using the substance can weaken his body to resist the effect that substance.
A crucial condition for molecular biology investigations is the extraction of high-quality genomic deoxyribonucleic acid (DNA) from plants. A good genomic DNA methodology should be simple, quick, and inexpensive, with a high yield and purity. Because polyphenols, polysaccharides, and secondary metabolites are present in some plants, DNA extraction becomes a hard, complicated, and time-consuming process. To isolate DNA from climate tolerant pearl millet leaf tissues with larger amounts of polysaccharides, we used a modified approach based on the cetyl trimethyl ammonium bromide (CTAB) method. It also prohibits the use of high-cost chemicals and equipment such as proteinase K, liquid nitrogen, and tissue lysers. DNA is extracted using a buffer (pH 8.0) containing 200 mM Tris-HCl, 20 mM ethylenediamine tetracetic acid (EDTA), 1.4 M NaCl, 2 percent CTAB, 2 percent sodium dodecyl sulphate (SDS), and 1.0 percent -mercaptoethanol, then purified using phenol, chloroform, isoamyl alcohol, and finally precipitated with sodium acetate and iso Using this procedure, good quality genomic DNA with crisp and clear bands was recovered from 48 pearl millet genotypes. The DNA yield ranged between 105.2 and 328.3 ng/l. Based on the absorbance at A260/A280 ratio, the purity of the DNA sample ranged from 1.74 to 1.95, suggesting that it was devoid of ribonucleic acid (RNA) and protein contamination. Simple sequence repeat (SSR) primers produced consistent and reliable amplification results ranging from 150 to 650 kb during PCR analysis. This research demonstrates a rapid, easy, efficient, specific, reproducible, reliable, and cost-effective method for extracting DNA from small to large numbers of plant samples that may be amplified by PCR and kept for prolonged periods of time.
Please see the link :- https://www.journalijecc.com/index.php/IJECC/article/view/30287
Keywords :- CTAB, genomic DNA extraction ,Pennisetum glaucum ,polyphenols, polysaccharides ,secondary metabolites.
If so why don't more people do it because swallowing this poison is a fucking nightmare.
I prob still won't touch this ever again because I've done a lot of healing since recovering from this addiction but I dont know enough to refute or confirm his claim.
About a week ago, I had all of my teeth pulled. I've been on the typical diet for someone without teeth that's healing.
For the last couple days, every time I eat something I feel nauseous. It's something about the consistency. For whatever reason, my body is like "nope, this mush isn't working for me" and I feel nauseous or I gag.
Did this happen with anyone else?
Also, I was not given immediate dentures so I'm gumming it for a few weeks until I get my impressions done.
I've been living on ensure and protein milkshakes for 9 days now. First he said full liquid diet till he removed the stitches from the lower ones. When he tooked them, he left me on 3 more days of liquid diet. It is supposed to end tomorrow, I guess.
I just wanted some liquid yogurt but realized it was from berries, so I strained it but still noticed some seeds and just drank it bc i'm starting to think he is crazy. I feel fine. But now I'm paranoid those seeds went into the hole. I have a tiny hole, more towards like in the inner side of the cheeks (not really were i thought it would be), that the stitches didn't apparently manage to close on the left side and have no idea how deep does it go. The entrance is tiny. On the other side I don't see any. It's either even more tiny or good at hidding.
I wasn't given any syringe or instructions on how to use one and was advise against salt mouth wash.
What is going to happen to the possible berry seeds in there?
Thanks for reading.
I heard that there is a way to tap a peyote and extract small quantities of the liquid over time. Is this a commonly used technique? Have you ever heard of it? I didn't find anything on the internet. Do you know anything about a method to extract the mescaline from the peyote without cutting off a part?
Hello everyone,
I'm looking for a source for liquid-liquid extraction in which more than one compound is transferred between the liquid medias. Can anyone recommend a source for it?
Hey there,
So I tried Paul Staments blue Juice extraction a few days ago, with chopped frozen mushrooms as extraction material. As expected they were already blueing like crazy. I covered them with water before adding ice cubes to prevent unnecessary oxidation and put everything in the fridge. After the ice cubes melted I was left with a pretty vile greenish brown liquid. Anyone with similar results who can say something about potency? Or does someone maybe know the chemistry and can tell me, what the colour means?
Next time I'll add some lemon juice as an active antioxidant right from the start and use destilled water
Edit: Didnt do anything. Next time I'll try fresh mushrooms without freezing. My guess is there are oxidising enzymes in the mushrooms and freezing them brings the enzymes into contact with the psilocybin/psilocin
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