Elicitor Puns

Microbial genomes harbor an abundance of biosynthetic gene clusters, but most are expressed at low levels and need to be activated for characterization of their cognate natural products. In this work, we report the combination of high‐throughput elicitor screening (HiTES) with Matrix‐Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI‐MS) for the rapid identification of cryptic peptide natural products. Application to Streptomyces ghanaensis identified amygdalin as an elicitor of a novel non‐ribosomal peptide, which we term cinnapeptin. Complete structural elucidation revealed cinnapeptin as a cyclic depsipeptide with an unusual 2‐methyl‐cinnamoyl group. Insights into its biosynthesis were provided by whole genome sequencing and gene deletion studies, while bioactivity assays showed antimicrobial activity against Gram‐positive bacteria and fission yeast. MALDI‐HiTES is a broadly applicable tool for the discovery of cryptic peptides encoded in microbial genomes

https://ift.tt/33RJMSd

http://www.ncbi.nlm.nih.gov/pubmed/19400727 would be very useful for my dissertation if anyone has it.

I don't want to step on anybody's toes here, but the amount of non-dad jokes here in this subreddit really annoys me. First of all, dad jokes CAN be NSFW, it clearly says so in the sub rules. Secondly, it doesn't automatically make it a dad joke if it's from a conversation between you and your child. Most importantly, the jokes that your CHILDREN tell YOU are not dad jokes. The point of a dad joke is that it's so cheesy only a dad who's trying to be funny would make such a joke. That's it. They are stupid plays on words, lame puns and so on. There has to be a clever pun or wordplay for it to be considered a dad joke.

Again, to all the fellow dads, I apologise if I'm sounding too harsh. But I just needed to get it off my chest.

Do your worst!

I'm surprised it hasn't decade.

For context I'm a Refuse Driver (Garbage man) & today I was on food waste. After I'd tipped I was checking the wagon for any defects when I spotted a lone pea balanced on the lifts.

I said "hey look, an escaPEA"

No one near me but it didn't half make me laugh for a good hour or so!

Edit: I can't believe how much this has blown up. Thank you everyone I've had a blast reading through the replies 😂

It really does, I swear!

Buenosdillas

They’re on standbi

Pilot on me!!

SAG's Open Access Cannabis Links

last update: 15 JAN 2022 (bit of clean up)

• SAG's Plant Lighting Guide

• Main open access page -many hundreds more papers

• Most links are for THC cannabis but some are for hemp or CBD and found primarily using Google Scholar. At the very bottom is a recently added section which will likely be every few months.

• Please report dead links! You sometimes have to look around a bit to find the actual PDF download in the page linked to.

Highlights

• Characterization of Nutrient Disorders of Cannabis sativa -this is for vegetative hemp and the only broad study I know of with nute issues and any cannabis

• Cannabis lighting: Decreasing blue photon fraction increases yield but efficacy is more important for cost effective production of cannabinoids "As percent blue increased from 4 to 20%, flower yield decreased by 12.3%. This means that flower yield increased by 0.77% per 1% decrease in blue photons."

Cannabis spectral and lighting

--THESIS

• The Impact of LED Spectra on Cannabis sativa Production master thesis

• SPECTRAL DIFFERENTIATION OF CANNABIS SATIVAL FROM MAIZE USING HYPERSPECTRAL INDICES master thesis

--ULTRAVIOLET

• Cannabis Yield Increased Proportionally With Light Intensity, but Additional Ultraviolet Radiation Did Not Affect Yield or Cannabinoid Content

• Cannabis Inflorescence Yield and Cannabinoid Concentration Are Not Improved with Long-Term Exposure to Short-Wavelength Ultraviolet-B Radiation

• [Cannabis sativa L. Response to Narrow Bandwidth UV and the Combination of Blue and Red Light during the Final Stages of Flowering on Leaf Level Gas-Exchange Parameters, Secondary Metabolite Production, and Yield](ht

Dad jokes are supposed to be jokes you can tell a kid and they will understand it and find it funny.

This sub is mostly just NSFW puns now.

If it needs a NSFW tag it's not a dad joke. There should just be a NSFW puns subreddit for that.

Edit* I'm not replying any longer and turning off notifications but to all those that say "no one cares", there sure are a lot of you arguing about it. Maybe I'm wrong but you people don't need to be rude about it. If you really don't care, don't comment.

When I got home, they were still there.

What did 0 say to 8 ?

" Nice Belt "

So What did 3 say to 8 ?

" Hey, you two stop making out "

I won't be doing that today!

[Removed]

You take away their little brooms

This morning, my 4 year old daughter.

Daughter: I'm hungry

Me: nerves building, smile widening

Me: Hi hungry, I'm dad.

She had no idea what was going on but I finally did it.

Thank you all for listening.

There hasn't been a post all year!

Nothing, he was gladiator.

Why

It’s pronounced “Noel.”