Microscopy Puns

Hey does anyone know if TMF Microscopy is a trustworthy site? I'm thinking of buying some spores from them but I want to see if anyone can confirm they're not going to scam me.

I get fluorescence idea where something with a lot of conjugations absorbs light at longer wavelength and then emits light at lower wavelength. Why aren't the absorbance and emission same? Where does energy stay after being absorbed that accounts for difference with emission

Hi I just wanted to take a moment to wish all of you a Happy New Years! 🤙 With that being said I'm gonna do a flash giveaway that all the active folks will hit the jackpot on..... 2 prizes....2winners..... swabs and prints 1-20 first 2 comments to guess the number or closest to it will win this NEW YEAR GIVEAWAY! WELCOME 2022!!!🔬🍄🤙❤️! Annnnnnnnnnd go!

Hi,

I am trying to detect which parts of a microscopy image are out of focus and how far (from z-stacked images). Sadly I only have data that is not annotated. I have focus values for the main focus plain but some of the objects that should be in focus are not always on that plane. I also have some annotated data with these objects (possibly later on also annotations on which plane is in focus).

What do you think is the best approach for this kind of problem. I thought of multiple approaches:

1. Using the main focus plane but hand annotating/using objects in focus to override the focus plane on these places
2. Hand labelling some images (maybe 100 to 1000) and
   1. training a model on these,
   2. then using that model to auto annotated some images with the trained model (for example annotate 1000 images manually and 1000 with the trained model)
   3. now train again on the combined set of images. Use the now trained model to relabel the automatic annotated images + some more.
3. Using some approach like Snorkel where annotated objects and focus of the whole image focus plane are used as weak supervision signals.

What do you think of my approaches (as of now I favour approach 2 or a combination of this with either 1 or 3)?

Also do you have any recommended papers that might help me out here?

Thanks in advance!

I finally got a nice microscope and would love to wow the kids and hopefully spark the interest. Any idea where I can find cool living specimens in the house? Everything outside is frozen.

I bought some sample slides on Amazon and they are nice but nothing is alive and moving.

Thank you

Can you just point a phone camera into the eye of the microscope and do it that way if you're on a really tight budget? Would anything be visible?
Otherwise what are the most affordable ones with good image/video capturing capability?

25€ per sp. Accepting payment in crypto only!

Shipping world wide!

Ps. aucklandiae (print)

Ps. ovoideocystidiata (print)

Ps. subaeruginosa (print)

Ps. subsecotioides (swab set)

Ps. weraroa (swab set)

Gym. luteofolius (swab set)

Gym. purpuratus (swab set)

The title is pretty much self-explanatory. I want to learn more about modern microscopy like light microscopy, confocal microscopy, point spread function. Like all, there is to know without being able to take a university class. So I was looking for a MOOC or some youtube tutorial online. If anyone knows something about such resources, that will be really helpful. Thanks in advance.

Just trying to observe spores up close and am wondering if I'll need a stronger magnification than 1000X.

I've found several organisms like this, varying in size 8-15 μm. They were in the enrichment culture from a soil sample treated beforehand with 100 μl trimethoprim, from which I then isolated Pseudomonas fluorescens. Inside, there were very dynamic vesicles (in size very similar to Pseudomonas itself, size 1-2 μm). I am really curious what this could be and I'd be grateful for any hints :)

https://preview.redd.it/pieaacs758c81.jpg?width=3120&format=pjpg&auto=webp&s=05d26f080646dca0d53e6fd785d06648d35b574c

Ooo we got some good ones in!!