Pivoting to bone-marrow derived multipotent adult progenitor cells (MAPCs) in a different model of prolonged cold ischemia, this alternate cell therapy was considered within the setting of EVLP in human donor lungs [34]. After 4 h of EVLP, there was less overall inflammation in treated organs as assessed by histology and by a decreased number of neutrophils and eosinophils. Another study of MAPCs to treat warm ischemia instead found that porcine lungs treated at the start of 6 h of EVLP had fewer cytokines and neutrophils in BAL [35]. There were, however, no changes to physiologic measures including PVR, compliance or PaO2/FiO2Β ratio compared to controls.
The MSC effect on the increase or decrease of key cytokines varies across publications. Mordant et al. reported a rise in IL-8 within their control group that did not exist in the MSC group eventually labeled as the βoptimal doseβ [31]. The findings of Nakajima et al. were more expansive with higher levels of HGF and IL-4, but lower levels of IL-12, IL-18 and IFN-y in the MSC-treated group [32]. In this study, however, there was no difference in IL-8 after EVLP as Mordant et al. had observed. A decrease in TNF-Ξ± was only observed following transplantation, but not EVLP. Similarly, Lee et al.βs work did not find decreased pro-inflammatory IL-8 or TNF-Ξ± [25]. Only in the study of MAPCs in EVLP was a higher level of IL-10 found in cell therapy treatment compared to vehicle control [34]. Many of the studies were limited by relatively small sample sizes, leading to the implication that continued study of the interplay between cell treatment and cytokine levels could shed light on expected changes in concentrations. To this end, NykΓ€nen et al. undertook an effort to gain greater control over cytokine manipulation, specifically by the genetic modification of MSCs to produce increased amounts of anti-inflammatory IL-10 [36]. These cells were then given at the start of 12 h of EVLP to 5 human lungs rejected for transplantation, with the finding that this translated to higher IL-10 in the EVLP perfusate and tissue. There were not, however, differences in PVR, oxygenation capacity and compliance, or other measures of pulmonary function.
https://www.mdpi.com/2073-4409/11/1/91/htm
ADSORPTION OF VOLATILE ORGANIC COMPOUNDS ON DIFFERENT TIMBER SPECIES Marco Vasconcelos1, Nereida Cordeiro1,2* Β Due to the health issues associated to VOC, this work aims to study the ads.
