"The Lytic cycle is when a phage injects nuclear material into preexisting bacterial DNA, multiples and then lyses the cell, releasing the new viruses"
how is this definition any different from the lysogeny cycle? I've been stuck on these two concepts forever, can someone please clear up their differnce?
Hello, i'm working on how lysogeny of Stx phage affects the metabolism of E.coli. Unfortunately, we haven't been able to isolate stable lysogens. So i was wondering if anyone could give me some advise on how to grow so stable lysogens, starting from which medium to use, supplements i could add, growth temperature or stuff like this.
Info:
We are using LB agar. We obtained the lysogens in the bacteria by creating lysys plaques, and isolating single colonies. We put those colonies to grow at 37° and we confirmed the presence of stx toxin through PCR and Colony Blot. Even the PCR positive colonies, lost the phage after a while. Since we are trying to see the effects of the phage of metabolism, adding an antibiotic resistance gene in the virus is not an option.
Thank you for your time
measuring it out always ruins my day
all my homies hate LB broth powder
I don't want to step on anybody's toes here, but the amount of non-dad jokes here in this subreddit really annoys me. First of all, dad jokes CAN be NSFW, it clearly says so in the sub rules. Secondly, it doesn't automatically make it a dad joke if it's from a conversation between you and your child. Most importantly, the jokes that your CHILDREN tell YOU are not dad jokes. The point of a dad joke is that it's so cheesy only a dad who's trying to be funny would make such a joke. That's it. They are stupid plays on words, lame puns and so on. There has to be a clever pun or wordplay for it to be considered a dad joke.
Again, to all the fellow dads, I apologise if I'm sounding too harsh. But I just needed to get it off my chest.
Do your worst!
I'm surprised it hasn't decade.
For context I'm a Refuse Driver (Garbage man) & today I was on food waste. After I'd tipped I was checking the wagon for any defects when I spotted a lone pea balanced on the lifts.
I said "hey look, an escaPEA"
No one near me but it didn't half make me laugh for a good hour or so!
Edit: I can't believe how much this has blown up. Thank you everyone I've had a blast reading through the replies 😂
It really does, I swear!
Buenosdillas
They’re on standbi
Pilot on me!!
Dad jokes are supposed to be jokes you can tell a kid and they will understand it and find it funny.
This sub is mostly just NSFW puns now.
If it needs a NSFW tag it's not a dad joke. There should just be a NSFW puns subreddit for that.
Edit* I'm not replying any longer and turning off notifications but to all those that say "no one cares", there sure are a lot of you arguing about it. Maybe I'm wrong but you people don't need to be rude about it. If you really don't care, don't comment.
When I got home, they were still there.
What did 0 say to 8 ?
" Nice Belt "
So What did 3 say to 8 ?
" Hey, you two stop making out "
I won't be doing that today!
[Removed]
You take away their little brooms
This morning, my 4 year old daughter.
Daughter: I'm hungry
Me: nerves building, smile widening
Me: Hi hungry, I'm dad.
She had no idea what was going on but I finally did it.
Thank you all for listening.
There hasn't been a post all year!
It’s pronounced “Noel.”
After all his first name is No-vac
What, then, is Chinese rap?
Edit:
Notable mentions from the comments:
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Spanish/Swedish/Swiss/Serbian hits
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French/Finnish art
-
Country/Canadian rap
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Chinese/Country/Canadian rock
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Turkish/Tunisian/Taiwanese rap
