Sorry about all the posts I've been making. It's hard to find reliable information online about treating dropsy.
On Thursday, I will have completed a two-week treatment of kanamycin/kanaplex for my betta fish. She is not getting worse, but she has not consistently improved. She still has a great appetite and a lot of energy. But she has pineconing on her whole body and dull coloring.
I just started doing Epsom salt baths yesterday and I'm going to try to do that 1-2x a day. I also have aquarium salt in the quarantine tank at about 1/2tsp per gallon. I've been changing about 25% of the water daily, give or take.
Is there anything else I can treat her with after kanamycin? I am worried about doing another antibiotic; I don't want to create antibiotic resistant bacteria.
making kanamycin broth for growing bacteria but sigma has like 500 different products with different prices. Does anyone know the difference between them?
Like these two? https://www.sigmaaldrich.com/catalog/product/SIGMA/K1377?lang=en®ion=US https://www.sigmaaldrich.com/catalog/product/SIAL/K1637?lang=en®ion=US
Thanks for the help!
Hey guys, I was wondering if it is possible to inoculate LB media and store the samples at 4 degrees for 24 hours, then grow them overnight to be used the following day? Reason being I can't get into the lab tomorrow, but I don't want to lose a day of work on Friday due to a lack of grown culture. I'm growing these cultures to miniprep, and have someone in the lab who is willing to move my cultures to the shaker from the fridge tomorrow. Any suggestions would be greatly appreciated!
I have a problem with my left eye and the doctor prescribed me Kanamycin Sulfate H (that's what is writen on the box). It's kind of like a cream that I need to put in my eye. I also take 2 others types of eye drops but this is the only antibiotic. Can I drink alcohol?
Just an FYI for anyone that is in the horrible spot I was in the past month. I had a pretty bad columnaris outbreak in a 55g tank that houses mostly guppies but also a healthy community of crystal reds. I bought the CRS on reddit about 6 months ago as juvies and they were just beginning to take off. Breeding etc.
Well- the columnaris was doing an absolute job on my guppie population (neons as well). I lost about 70% of the fish I had. I knew it had to be treated but all of the treatments call for Furan-2 and Kanamycin. I couldn't find ANY solid info on Furan-2. Some posts said it would kill shrimp etc.
Just got done with essentially a week of heavy treatment and the CRS are doing absolutely fine. Didn't lose one. Babies are all good. Guppies are healthy again. Everyone is happy.
Furan-2 was safe for me.
20ug/mL gave me growth from untransformed dh5a. 50 ug/mL didn't give me any transformed colonies. Tips for transforming on kanamycin plates?
Hey folks, I've been having some trouble with the mutagenesis process using a kanamycin-resistant vector. In my amp-containing vectors, the mutagenesis, transformation of mutagenic PCR products, and miniprepping of that transformation all works great but when I use my Kan vector I get very few or small transformant colonies and poor, impure miniprep yield. I've been using 50 ug/mL kan, and I just started one using 25, so we'll see if that helps. Have any of you had a similar problem, and how were you able to fix it?
We're having trouble getting our plasmid after a transformation and think that LB Agar kanamycin plates made in November of last year may be the problem.
Please post sources for your info, thanks!!
And do you have any idea why the plants might look like this?
Basically what I'm seeing is that the dicotyledons are dark green (instead of yellow like they are being fully susceptible), but the first pair of true leaves is yellowish and doesn't look healthy.
Fully resistant plants should be dark green on all leaves. You can see a better example of what I'm talking about when I zoom in - there are a few totally resistant plants in there, and then a bunch with the weird half-resistance.
Kanamycin resistance isn't dose dependent so this isn't a case of some of the plants being heterozygous and some being homozygous.
This is a T2 generation, which means 75% of the plants should be resistant to kanamycin. As you can see from the first image, most of the plants have this weird paleness on the second pair of leaves, some (<25%) look like fully resistant and a few (one or two) are fully susceptible.
I have 5 plates out of 10 that look like this, one plate that had the proper 75-25 proportions of living:dead, but the living plants still mostly had the yellowness on the true leaves. The remaining plates mostly didn't germinate; the seeds that did were fully resistant. (I think that's a problem with my seeds, not the plates).
Is it a nutrient deficiency in my agar medium? We're using MS salts, sucrose, and agar in the recommended proportions. Our salts are ~ 2 years old and have been stored in a dessication box in the fridge.
Thank you so much if you can help me! This has been driving me insane for a month or two now.
I'm pretty sure that two of my bettas have the slower growing variety of columnaris. The problem is trying to figure out how to treat it. Seachem wants me to dose the food, but one is a very poor eater and I'm not certain this is going to work.
Any suggestions on dosing with Furan and Kanamycin. I'm seeing so much information I'm having trouble making sense of it all.
Do your worst!
For context I'm a Refuse Driver (Garbage man) & today I was on food waste. After I'd tipped I was checking the wagon for any defects when I spotted a lone pea balanced on the lifts.
I said "hey look, an escaPEA"
No one near me but it didn't half make me laugh for a good hour or so!
Edit: I can't believe how much this has blown up. Thank you everyone I've had a blast reading through the replies 😂
It really does, I swear!
They’re on standbi
Pilot on me!!
Buenosdillas
Dad jokes are supposed to be jokes you can tell a kid and they will understand it and find it funny.
This sub is mostly just NSFW puns now.
If it needs a NSFW tag it's not a dad joke. There should just be a NSFW puns subreddit for that.
Edit* I'm not replying any longer and turning off notifications but to all those that say "no one cares", there sure are a lot of you arguing about it. Maybe I'm wrong but you people don't need to be rude about it. If you really don't care, don't comment.
When I got home, they were still there.
What did 0 say to 8 ?
" Nice Belt "
So What did 3 say to 8 ?
" Hey, you two stop making out "
I won't be doing that today!
You take away their little brooms
This morning, my 4 year old daughter.
Daughter: I'm hungry
Me: nerves building, smile widening
Me: Hi hungry, I'm dad.
She had no idea what was going on but I finally did it.
Thank you all for listening.
There hasn't been a post all year!
