Substrate specificity and proposed structure of the proofreading complex of T7 DNA polymerase sciencedirect.com/science…
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πŸ‘€︎ u/MarcHerb
πŸ“…︎ Jan 27 2022
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God is inside us... in the form of DNA polymerase who has very thick eyeglasses.
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πŸ‘€︎ u/Born-Process-9848
πŸ“…︎ Nov 23 2021
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Likelihood of needing to know the functions of different DNA polymerases?

Or is it just one of those things that I should memorize because it will come up in M1 and M2 anyways?

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πŸ‘€︎ u/LLegwarmers91
πŸ“…︎ Jan 06 2022
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DNA Tranlession Polymerase in Prokaryotic Cells

DNA Tranlession Polymerase in prokaryotic cells: History, structures and function Soheil Malekpour DNA is one of the most important part of the cell that gives cell integrity and character. This pa.

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πŸ‘€︎ u/Ok-Pass-2435
πŸ“…︎ Jan 22 2022
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Sinclair tweeted this study, saying, "DNA mutations cause cancer but do not appear to cause aging in humans." Increased somatic mutation burdens in normal human cells due to defective DNA polymerases | Nature Genetics nature.com/articles/s4158…
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πŸ‘€︎ u/newbooke
πŸ“…︎ Oct 01 2021
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Learn DNA Primer Design for Polymerase Chain Reaction idownloadcoupon.com/coupo…
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πŸ‘€︎ u/smartybrome
πŸ“…︎ Dec 22 2021
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Dna polymerases other names?

Hi so I’m kinda confused with other names of DNA Polymerase iii and DNA Polymerase i, cause like in my lecture, the lecturer calls it DNA Polymerase iii and DNA Polymerase i, but in the assignment it is called DNA Polymerase alpha, delta, epsilon. Sorry if its a dumb question cause I can’t think of a good search on googleπŸ˜”

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πŸ“…︎ Dec 13 2021
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How does DNA polymerase I remove the RNA primers in okazaki fragments during DNA replication?

Primase adds RNA primers in the beginning of each okazaki fragment and then DNA pol. III adds DNA bases until the RNA primer of the next fragment. After they're joined together by DNA ligase and the primers need to be removed, does DNA pol. I just remove uracil and add adenine IF there happens to be any in the RNA primers? Or does it remove the whole RNA primer? If it does remove it all, 1) why? Apart from uracil, the rest of the sequence set by the primase should be correct, right? 2) how would it know exactly which 10 nucleotides were set by the primase? Bonus question: is exonuclease an enzyme that's part of DNA pol. I ? Or a seperate enzyme?

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πŸ‘€︎ u/babyboyjunmyeon
πŸ“…︎ Oct 25 2021
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Why is DNA polymerase needed to make a cDNA plasmid?

Passage link (question 4):https://jackwestin.com/daily/mcat-practice-passages/biological-biochemical-practice-passages/signaling-intermediates

https://preview.redd.it/lxipu1xrqwu71.png?width=1236&format=png&auto=webp&s=9b385e3dc92aa02b47ffee20ddae3185765c309d

I found this online: "Reverse transcriptase contains three enzymatic activities: (1) RNA-dependent DNA polymerase, (2) RNase H, and (3) DNA-dependent DNA polymerase. First, RNA-dependent DNA polymerase synthesizes a DNA strand complementary to the RNA template. Then RNase H removes the RNA strand from the RNA–DNA hybrid double helix. Then the DNA-dependent DNA polymerase completes double-stranded DNA synthesis."

So if RT has activity (3), why is DNA polymerase needed in cDNA plasmid formation?

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πŸ‘€︎ u/POJJERZ
πŸ“…︎ Oct 22 2021
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What is the word for the fact that Polymerase doesn't easily disassociate from DNA?

There is a word for this that I forgot and cannot seem to find. Polymerase activity is _______, which means that it doesn't easily fall off the DNA strand and continues for a long time along the DNA strand. It's something along the lines of "persistent" or "robust"

PROCESSIVITY that's the word!

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πŸ‘€︎ u/horsehooves101
πŸ“…︎ Nov 25 2021
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Bruh has anyone else noticed all the conflicting information about euk DNA polymerases

Like between the Kaplan book and the internet... this shit got me heated. This isn't my job dude

https://preview.redd.it/n302lsfy4ar71.png?width=1392&format=png&auto=webp&s=1dbb3cf60abbe303d6744a922bf499046ba4a376

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πŸ‘€︎ u/orc-asmic
πŸ“…︎ Oct 03 2021
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Learn DNA Primer Design for Polymerase Chain Reaction idownloadcoupon.com/coupo…
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πŸ‘€︎ u/smartybrome
πŸ“…︎ Oct 04 2021
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Please don't blame DNA Polymerase.
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πŸ‘€︎ u/AniPsych0908
πŸ“…︎ May 30 2021
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Can there be several RNA polymerases transcribing RNA from DNA during transcription?
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πŸ‘€︎ u/oldnips
πŸ“…︎ Sep 29 2021
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Site‐Specific 5‐Formyl Cytosine Mediated DNA‐Histone Cross‐Links: Synthesis and Polymerase Bypass by Human DNA Polymerase Ξ·

DNA-Protein Cross-links (DPCs) between DNA epigenetic mark 5-formylC and lysine residues of histone proteins spontaneously form in human cells. Such conjugates are likely to influence chromatin structure and mediate DNA replication, transcription, and repair, but are challenging to study due to their reversible nature. Here we report the construction of site specific, hydrolytically stable DPCs between 5fdC in DNA and K4 of histone H3 and an investigation of their effects on DNA replication. Our approach employs oxime ligation, allowing for site-specific conjugation of histones to DNA under physiological conditions. Primer extension experiments revealed that histone H3-DNA crosslinks blocked DNA synthesis by hPol Ξ· polymerase, but were bypassed following proteolytic processing.

https://ift.tt/3AArS2Y

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πŸ‘€︎ u/TomisMeMyselfandI
πŸ“…︎ Oct 12 2021
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