Hello, I'm wondering if anyone knows how pen/strep would affect collagenase type 1 activity? I am doing a collagenase activity assay on a collagenase solution with pen/strep.
Would it be possible to tenderize meat using some enzyme?
As i understand it collagen is what makes meat tough so could you use an enzyme to break it down to some degree, and is this why people use pinapples to tenderize meat?
Hi Labrats! I need to reconstitute collagenase IA lyophilized powder to prepare a digestion buffer for isolating tumor cells from breast samples. The manufacturer (Sigma) advises to use Krebs Ringer buffer with calcium and BSA or in Hank's Balanced Salts for tissue culture. However I've seen a protocol that uses PBS instead. Would you recommend doing so? Also, do you follow any special safety measures besides wearing gloves and working in a laminar flow hood?
I also have to reconstitute hyaluronidase for the same purpose. The product information sheet advises 20 mM sodium phosphate buffer, pH 7.0, with 77 mM sodium chloride and 0.1 mg/ml BSA at 1 mg/ml. Would it be the same to use just PBS?
Thanks in advance!
Hi all,
Am I reading these product sheets wrong? 1 and A seem like they're identical, 2 and B look identical, etc...
I've been told these are FAR from being the same... What gives?
Hi labrats, I'm looking to use collagenase D for my experiment. I reconstitute it in Hank's (minus calcium and magnesium), and the TDS from Sigma-Aldrich says you can only store it in this form up to a week at -15 to -25 degrees Celsius before it loses most of its activity (PDF link: http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Roche/Bulletin/1/colldrobul.pdf).
The enzyme is shipped in a dry powder at 100 mg. The problem is, it's troublesome and imprecise to reconstitute it unless I weigh out at least 25 mg or so to use, and I don't want to have to reorder the enzyme every month or so (it's shared with other lab members). Any suggestions?
Thanks in advance.
I don't want to step on anybody's toes here, but the amount of non-dad jokes here in this subreddit really annoys me. First of all, dad jokes CAN be NSFW, it clearly says so in the sub rules. Secondly, it doesn't automatically make it a dad joke if it's from a conversation between you and your child. Most importantly, the jokes that your CHILDREN tell YOU are not dad jokes. The point of a dad joke is that it's so cheesy only a dad who's trying to be funny would make such a joke. That's it. They are stupid plays on words, lame puns and so on. There has to be a clever pun or wordplay for it to be considered a dad joke.
Again, to all the fellow dads, I apologise if I'm sounding too harsh. But I just needed to get it off my chest.
Do your worst!
I'm surprised it hasn't decade.
For context I'm a Refuse Driver (Garbage man) & today I was on food waste. After I'd tipped I was checking the wagon for any defects when I spotted a lone pea balanced on the lifts.
I said "hey look, an escaPEA"
No one near me but it didn't half make me laugh for a good hour or so!
Edit: I can't believe how much this has blown up. Thank you everyone I've had a blast reading through the replies 😂
It really does, I swear!
Buenosdillas
They’re on standbi
Pilot on me!!
Dad jokes are supposed to be jokes you can tell a kid and they will understand it and find it funny.
This sub is mostly just NSFW puns now.
If it needs a NSFW tag it's not a dad joke. There should just be a NSFW puns subreddit for that.
Edit* I'm not replying any longer and turning off notifications but to all those that say "no one cares", there sure are a lot of you arguing about it. Maybe I'm wrong but you people don't need to be rude about it. If you really don't care, don't comment.
When I got home, they were still there.
What did 0 say to 8 ?
" Nice Belt "
So What did 3 say to 8 ?
" Hey, you two stop making out "
I won't be doing that today!
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