[9th Grade Biology] What will happen to the human cell membranes if the cytosol was changed to a non-polar liquid?

To be specific I mean the structure of the human cell

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πŸ‘€︎ u/Used_Homework290
πŸ“…︎ Oct 28 2021
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Which type of amino acids ( Polar/NonPolar ) are more likely to be found on the interior of a globular protein that exists in the cytosol of a cell.

I have never taken a biology class and jumped straight into ap bio this year. I was doing well in chem and jump into ap chem and was told to take this class as well. Admittedly I am confused on a few aspects as reading through some things didn't really help me. Are there any good guides or youtube videos that can explain a bit more on concepts?

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πŸ‘€︎ u/Shadowslasher-20
πŸ“…︎ Sep 27 2021
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Increased levels of mitochondrial import factor Mia40 prevent the aggregation of polyQ proteins in the cytosol doi.org/10.15252/embj.202…
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πŸ‘€︎ u/MarcHerb
πŸ“…︎ Aug 18 2021
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Absolute Quantification of Drug Vector Delivery to the Cytosol

Macromolecular drug delivery vectors must translocate at the plasma membrane or escape from endosomes to reach the cytosol. This very inefficient process requires optimization, which is hindered by the difficulty to accurately measure cytosolic arrival. An exceptionally sensitive and robust assay to quantify this step was developed. The assay was validated by determining the absolute numbers of two macromolecular vectors reaching the cytosol.

Abstract

Macromolecular drugs inefficiently cross membranes to reach their cytosolic targets. They require drug delivery vectors to facilitate their translocation across the plasma membrane or escape from endosomes. Optimization of these vectors has however been hindered by the difficulty to accurately measure cytosolic arrival. We have developed an exceptionally sensitive and robust assay for the relative or absolute quantification of this step. The assay is based on benzylguanine and biotin modifications on a drug delivery vector of interest, which allow, respectively, for selective covalent capture in the cytosol with a SNAP-tag fusion protein and for quantification at picomolar sensitivity. The assay was validated by determining the absolute numbers of cytosolic molecules for two drug delivery vectors: the B-subunit of Shiga toxin and the cell-penetrating peptide TAT. We expect this assay to favor delivery vector optimization and the understanding of the enigmatic translocation process.

https://ift.tt/2Ps7LT7

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πŸ‘€︎ u/TomisMeMyselfandI
πŸ“…︎ May 29 2021
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Cell signaling: After that ca2+ ions have been released from ER into the cytosol, how do the ca2+ concentration go back to its resting state?
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πŸ‘€︎ u/mikediablo_
πŸ“…︎ Jun 30 2021
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My first amoeba that i found! Amoebas move around by creating these outward projections called pseudopodia (false feet). That's the same way our white blood cells move! Also you can see the cytosol and all the digested material move towards the pseudopodia. v.redd.it/16m09vbfzku61
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πŸ‘€︎ u/Sucuk420
πŸ“…︎ Apr 21 2021
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Does protein translation happen in the cytosol or in the rough ER?

I read that translation happens in the cytoplasm, but also read elsewhere that it happens in the rough ER. Which is it? Does it happen in the cytosol outside of all organelles, by ribosomes floating in the cytosol, or is it in the rough ER by the ribosomes on the ER?

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πŸ‘€︎ u/vbalasub
πŸ“…︎ Jun 10 2021
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Is it correct to say that the outerface of outermembrane is exposed to cytosol? Innerface of outermembrane is exposed to matrix? Outerface of inner membrane is exposed to intermembrane space? Innerface of inner membrane is exposed to matrix? TY
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πŸ“…︎ Apr 01 2021
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Dysfunctional SRPβ€”> accumulation of protein in cytosol (FA)
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πŸ‘€︎ u/SnooPeppers6545
πŸ“…︎ Apr 16 2021
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[ASAP] Regulation of Proteins to the Cytosol Using Delivery Systems with Engineered Polymer Architecture

Journal of the American Chemical SocietyDOI: 10.1021/jacs.1c00258

Jessica A. Kretzmann, David C. Luther, Cameron W. Evans, Taewon Jeon, William Jerome, Sanjana Gopalakrishnan, Yi-Wei Lee, Marck Norret, K. Swaminathan Iyer, and Vincent M. Rotello

https://ift.tt/30zdxnR

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πŸ‘€︎ u/TomisMeMyselfandI
πŸ“…︎ Mar 11 2021
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Does the Malate-aspartate shuttle go in both directions or just the one pictured? Ive seen some sources say that oxaloacetate is converted into malate in the matrix, which is then exported to the cytosol and converted back to oxaloacetate for gluconeogenesis (opposite arrows in picture).
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πŸ‘€︎ u/heydoyouseethat
πŸ“…︎ May 18 2020
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Spent an hour on this drawing (not a good drawer) believing that the stuff inside the cells were Nucleus. I didn’t read the instructions and turns out they were Cytosol...
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πŸ‘€︎ u/929yiyi
πŸ“…︎ May 15 2020
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Immunofluorescence Fixation Method for Cytosol Washout?

I'm trying to image a protein that has dual cytosolic and membrane-bound (via protein-protein interaction) localization, but I need to wash out or otherwise not see the cytosolic pool - I'm only interested in seeing the membrane-bound pool. I've tried using digitonin (0.025% in PBS for 10 seconds, then fixation in 4% PFA in PBS for 15 minutes at 37 ℃), but all of the cells (HeLas) ball up and die. I may try a lower digitonin concentration, but I'd like an alternative as well.

Would fixation in ice-cold methanol work for this? I need to be able to see the membrane-associated pool of my protein of interest, as well as a membrane-anchored protein marker.

Any advice on fixation methods for this would be much appreciated!

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πŸ‘€︎ u/proteomicsguru
πŸ“…︎ Nov 13 2019
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Effects of Polyhydric and Monohydric Compounds on the Stability of Type I Receptors for Adrenal Steroids in Brain Cytosol - PubMed pubmed.ncbi.nlm.nih.gov/2…
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πŸ‘€︎ u/FrigoCoder
πŸ“…︎ May 22 2020
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Cytosol/cytoplasm/extracellular space/intercellular/etc

This may seem silly but one of the things that I cannot ever get straight is confidently stating where a process takes place, or what is located within one of these spaces. I’m specifically talking about the cytosol, cytoplasm, ECS, ICS, etc. I seem to always forget after a couple weeks of getting a card correct.

Does anyone have a good explanation, or ways to remember how to differentiate?

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πŸ‘€︎ u/langerhauns
πŸ“…︎ Oct 30 2019
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Does it make difference? Cytoplasm vs Cytosol

Can someone explain me these cards please? https://imgur.com/a/i0LmTcQ and this https://imgur.com/a/g0roPVv Why differentiate this? TIA

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πŸ‘€︎ u/artek2001
πŸ“…︎ Nov 27 2019
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Cytosol and Lysosome would like to: *know your location*
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πŸ‘€︎ u/SirLlama17
πŸ“…︎ Feb 19 2019
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Absolute Quantification of Drug‐Vector Delivery to the Cytosol

Macromolecular drugs inefficiently cross membranes to reach their cytosolic targets. They require drug delivery vectors to facilitate their translocation across the plasma membrane or escape from endosomes. Optimization of these vectors has however been hindered by the difficulty to accurately measure cytosolic arrival. We have developed an exceptionally sensitive and robust assay for the relative or absolute quantification of this step. The assay is based on benzylguanine and biotin modifications on a drug delivery vector of interest, which allow respectively for selective covalent capture in the cytosol with a SNAP‐tag fusion protein and for quantification at picomolar sensitivity. The assay was validated by determining the absolute numbers of cytosolic molecules for two drug delivery vectors: The B‐subunit of Shiga toxin and the cell‐penetrating peptide TAT. We expect this assay to favour delivery vector optimization and the understanding of the enigmatic translocation process.

https://ift.tt/2Ps7LT7

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πŸ‘€︎ u/TomisMeMyselfandI
πŸ“…︎ Apr 27 2021
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Cytosol is just Cell Juice
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πŸ‘€︎ u/Epic_Meow
πŸ“…︎ Oct 05 2019
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