Bacterial growth curves for anaerobic (facultative) microbe?

Hi! I am planning on performing bacterial growth curve measurements under anaerobic conditions. I will be using the GasPak system. What is a good protocol flow to use for these types of experiments? I will be measuring both OD600 and spot plating.

Thanks!

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πŸ‘€︎ u/iineedacookie
πŸ“…︎ Oct 17 2021
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Comparative Analysis of Gut Microbial Communities in Children under 5 Years Old with Diarrhea. proportion of aerobic and facultative anaerobic microbes in infectious group was much higher than the non-infectious group. relative abundance of Enterococcus significantly higher in healthy control group jmb.or.kr/journal/viewJou…
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πŸ‘€︎ u/MaximilianKohler
πŸ“…︎ Apr 17 2018
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Can I activate a freeze-dried facultative anaerobe under anaerobic conditions?

Hi, I want to activate a freeze-dried actinobacillus succinogenes under anaerobic conditions, to avoid the overexpression of LDH. The problem is that they recommend to activate it under aerobic conditions and don't talk about if it's possible to do the same under anaerobic conditions. Is there any problem if I try to do it in anaerobiosis ?

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πŸ‘€︎ u/Lord_codfish
πŸ“…︎ Oct 01 2018
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Specific, but does anyone know how long Facultative Anaerobic bacteria lasts on surfaces like floor carpets, leather, and tiles?

I’m worried about Capnocytophaga Canimorsus specifically.

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πŸ‘€︎ u/konodioda879
πŸ“…︎ Apr 18 2020
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Increased D-Lactic Acid Intestinal Bacteria in Patients with Chronic Fatigue Syndrome: his study suggests a probable link between intestinal colonization of Gram positive facultative anaerobic D-lactic acid bacteria and symptom expressions in a subgroup of patients with CFS. iv.iiarjournals.org/conte…
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πŸ‘€︎ u/shallah
πŸ“…︎ Sep 23 2016
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le anaerobic organism has arrived
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πŸ‘€︎ u/YaBoiFulan
πŸ“…︎ May 30 2021
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Increased D-Lactic Acid Intestinal Bacteria in Patients with Chronic Fatigue Syndrome: This study suggests a probable link between intestinal colonization of Gram positive facultative anaerobic D-lactic acid bacteria & symptom expressions in a subgroup of patients with CFS researchgate.net/profile/…
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πŸ‘€︎ u/shallah
πŸ“…︎ Dec 30 2015
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Increased D-Lactic Acid Intestinal Bacteria in Patients with Chronic Fatigue Syndrome: This study suggests a probable link between intestinal colonization of Gram positive facultative anaerobic D-lactic acid bacteria & symptom expressions in a subgroup of patients with CFS researchgate.net/profile/…
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πŸ‘€︎ u/shallah
πŸ“…︎ Jan 01 2016
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[WP] Humans have achieved interstellar travel and are seeding planets across the galaxy with photosynthetic organisms to make the planets suitable for human colonies. The anaerobic life that live on these worlds don't take this act of attempted genocide lightly
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πŸ“…︎ May 17 2021
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Symbiosis can be obligate, where two organisms cannot survive without each other or facultative, where species live together by choice. They vary between mutualism (positive for both), commensalism (positive for 1, neutral for the other), and parasitism (positive for 1, negative for the other). en.wikipedia.org/wiki/Sym…
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πŸ‘€︎ u/FillsYourNiche
πŸ“…︎ Jan 29 2017
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checkmate anaerobic organisms
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πŸ‘€︎ u/nonnoxer
πŸ“…︎ Nov 08 2019
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Who would win between the only 2 anaerobic hive organism monsters? Destoroyah vs Hedorah

Destroroyah has the micro-oxygen beam which is deadly to us because we are aerobic organisms that depend on the target of the micro-oxygen beam oxygen.

>Herodah is composed almost entirely of a mineral called Hedrium, an acidic, sludge-like material, which he uses as his primary means of attack. Hedorah can launch pieces of his body at opponents, which will burn or smother them. Hedorah can also discharge large quantities of sludge without impacting his size in any way, shown in Godzilla vs. Hedorah when he attempts to drown Godzilla in a pit by dumping a continuous stream of sludge onto him.

-Wikizilla

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πŸ‘€︎ u/Non-profitboi
πŸ“…︎ Sep 12 2019
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Does fermentation occur in all anaerobic organisms?

Like if it can use a non-oxygen terminal electron acceptor, does that mean it doesn't have to ferment?

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πŸ‘€︎ u/JoeyBobBillie
πŸ“…︎ Apr 11 2020
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First time seeing this organism. Anaerobic Blood culture bottle.
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πŸ‘€︎ u/Labrechaun
πŸ“…︎ Nov 18 2018
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Chloroflexus islandicus is a photosynthetic bacterium isolated from the Strokkur Geyser in Iceland. This organism is thermophilic showing optimal growth at 55Β°C (131Β°F) with a pH range of 7.5 – 7.7. C. islandicus grows best photoheterotrophically under anaerobic conditions with light but is capable

Chloroflexus islandicus is a photosynthetic bacterium isolated from the Strokkur Geyser in Iceland. This organism is thermophilic showing optimal growth at 55Β°C (131Β°F) with a pH range of 7.5 – 7.7. C. islandicus grows best photoheterotrophically under anaerobic conditions with light but is capable of chemoheterotrophically growth under aerobic conditions in the dark. C. islandicus has a yellowish green color. The individual cells form unbranched multicellular filaments about 0.6 Β΅m in diameter and 4-7 Β΅m in length.

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πŸ‘€︎ u/KnightOfDoom22
πŸ“…︎ Oct 16 2019
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[MEME] im a facultative anaerobe
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πŸ‘€︎ u/deezpantshit
πŸ“…︎ Feb 19 2019
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Is there any organism which can survive long term on anaerobic respiration alone?

I know humans can't because our ATP needs are too high, but I was wondering if any organism is able to survive on anaerobic respiration. I'm guessing they'd be micro-organisms, and they'd probably have to be able to:

  • Survive off only 2ATP per glucose and still somehow have energy to go get that glucose

  • Have an efficient way of purging lactic acid (or whatever byproducts they have)

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πŸ‘€︎ u/JoseDzirehChong
πŸ“…︎ Mar 27 2019
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Would it be possible to put anaerobic organisms on a planet to help make it habitable for a larger human population?
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πŸ‘€︎ u/dtamura18
πŸ“…︎ May 15 2018
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Could an organism survive with anaerobic respiration?

Just as the title says, could an organism the size of a cat, derived from microorganisms, efficiently survive using only anaerobic respiration? If so what adaptations would it need?

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πŸ‘€︎ u/MagentaDinoNerd
πŸ“…︎ Sep 22 2018
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TIL that many famous pathogens, such as Salmonella, E. Coli, and Y. Pestis, are all Facultative Anaerobes, that is to say, they prefer to "breathe" oxygen, but can switch to fermentation in its absence en.wikipedia.org/wiki/Fac…
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πŸ‘€︎ u/Jozarin
πŸ“…︎ Sep 16 2019
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Why anaerobic organisms can not grow much bigger than, say, Ascaris? Are anaerobes able to evolve into a conscious life?

Maybe anaerobes of today can not get any bigger, complex and smarter than Ascaris because of the competition with aerobes, but what if the "Oxygen catastrophe" had never happened? Could anaerobe evolution produce big animals and, maybe, even into something smart?

If so, then there is a chance that "aliens" can be anaerobic, too, and hence the search for theoretically conscious life-applicable planets in the space should include atmospheres that are not oxygen-rich at all.

Thanks in advance.

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πŸ‘€︎ u/RRDDSS
πŸ“…︎ Apr 29 2017
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[Hig School Biology] Which organisms perform anaerobic respiration?

I can't believe such a simple fact is so infuriating to Google. I search, and there's no straight answer. It's just "here's some examples of what can, and some things can if there's no oxygen". Reworded it a few times, no dice.

So I google which CAN'T do anaerobic respiration. And I get some answers that say "some cells can't do anaerobic respiration, so instead they do fermentation". But isn't fermentation what anaerobic respiration is? I was taught 2 types of anaerobic respiration, being lactic acid fermentation, and alcohol fermentation.

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πŸ‘€︎ u/SYZekrom
πŸ“…︎ Apr 02 2017
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TIL that in many anaerobic organisms, including some animals, the hydrogenosome is the powerhouse of the cell en.wikipedia.org/wiki/Hyd…
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πŸ‘€︎ u/AOEUD
πŸ“…︎ Nov 09 2015
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Genomic Expansion of Domain Archaea Highlights Roles for Organisms from New Phyla in Anaerobic Carbon Cycling cell.com/current-biology/…
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πŸ‘€︎ u/burtzev
πŸ“…︎ Mar 16 2015
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Genomic Expansion of Domain Archaea Highlights Roles for Organisms from New Phyla in Anaerobic Carbon Cycling cell.com/current-biology/…
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πŸ‘€︎ u/burtzev
πŸ“…︎ Mar 16 2015
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Twelve egg-shaped anaerobic digesters on Deer Island break down our waste into methane gas, carbon dioxide, solid organic byproducts, and water (second largest sewage treatment plant in the U.S.)
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πŸ‘€︎ u/alanboston
πŸ“…︎ Jul 05 2021
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The first multi-cellular, anaerobic organism known to humans boingboing.net/2010/04/07…
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πŸ‘€︎ u/DrJulianBashir
πŸ“…︎ Apr 08 2010
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Incredibly preserved 2400 year old shipwreck compared to a contemporary depiction. It was found off the coast of Bulgaria. Bellow the Black Sea 200 meters /650feet it's completely anaerobic which prevents organic material from deterioration. It's also complete darkness, the image is photogrammetry.
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πŸ‘€︎ u/r3vange
πŸ“…︎ Jun 01 2021
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What is the difference between treaty and facultative reinsurance?

.

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πŸ‘€︎ u/dude_rulz
πŸ“…︎ Jan 30 2022
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Classic example of Armillaria and how it is a facultative parasite
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πŸ“…︎ Oct 16 2021
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"Facultative Parthenogenesis in California Condors", Ryder et al 2021 academic.oup.com/jhered/a…
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πŸ‘€︎ u/gwern
πŸ“…︎ Oct 28 2021
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New paper studying cut marks on 1.9 million year old animal bones finds evidence of primary usage by hominids, meaning that humans were hunting and butchering animals themselves, not just stealing other carnivores' kills. Discussion makes strong case for facultative & obligate carnivory in humans. /r/ketoscience/comments/p…
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πŸ‘€︎ u/dem0n0cracy
πŸ“…︎ Aug 10 2021
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La liaison facultative, should I do it?

Let me get this out there first, why the hell must you guys torture potatoes who are trying not to make a fool of themselves like me? Okay, now I have done ranting, do you guys recommend doing La liaison facultative like in the phrase <jeux intΓ©ressants> or <Nous sommes allΓ©s>? I get that it is a regional and optional thing but in general, is it better to learn it in order to understand others and speak in a clearer fashion?

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πŸ“…︎ Nov 06 2021
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Do anaerobic proccessed bean have alcohol?

I got an anaerobic proccessed bag of beans and after reading about them it says that they go through fermentation so i was asking myself if they contained alcohol, thanks

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πŸ“…︎ Dec 23 2021
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Anyone know of any roasters who offer the same coffee, roasted with different processes? Would love to try the same coffee as a washed vs honey vs anaerobic, etc. thanks !
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πŸ‘€︎ u/Lcphilly
πŸ“…︎ Jan 05 2022
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Would it be possible to put anaerobic organisms on a planet to help make it habitable for a larger human population?
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πŸ‘€︎ u/dtamura18
πŸ“…︎ May 16 2018
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are there any anaerobic multicellular organisms?
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πŸ‘€︎ u/wanabeswordsman
πŸ“…︎ Aug 24 2014
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