I made those solutions in August 2019 and haven’t used them since. They’ve been kept in a 4 degree fridge, should I make new solutions or would these be okay to use again soon?
I'm trying to eliminate a formaldehyde fixation step from a TEM preparation protocol. I visualize DNA (chromatin, not sections) using a sequential stain with phosphotungstic acid (1% in 75% EtOH) and uranyl acetate (1% in water without pH adjustment).
I don't seem to be able, however, to get good contrasting results when omitting the formaldehyde fixation step prior to contrasting.
Does anybody know if fixation with formaldehyde somehow inherently improves the uranyl acetate stain? Does pH of uranyl acetate matter that much? If so is there a way to solve that?
Thanks in advance for any tips.
I don't want to step on anybody's toes here, but the amount of non-dad jokes here in this subreddit really annoys me. First of all, dad jokes CAN be NSFW, it clearly says so in the sub rules. Secondly, it doesn't automatically make it a dad joke if it's from a conversation between you and your child. Most importantly, the jokes that your CHILDREN tell YOU are not dad jokes. The point of a dad joke is that it's so cheesy only a dad who's trying to be funny would make such a joke. That's it. They are stupid plays on words, lame puns and so on. There has to be a clever pun or wordplay for it to be considered a dad joke.
Again, to all the fellow dads, I apologise if I'm sounding too harsh. But I just needed to get it off my chest.
Hi Everyone
I am finishing up at the lab I did my graduate training in, my supervisor is aware that I am slowly getting into microscopy, and she has given me a list of her stain inventory and said I can take a small amount of whatever I want, to use at home. I am listing them below
I was hoping some of you can spend a few minutes browsing this list, and recommend any stains that I should take a small amount of, and make some stains for future use keeping the following criteria in mind:
I would like to use stains that are stable at room temperature for long periods of time, I can add some thymol to help curb microbe contamination
When I want to fix specimens, I will likely use heat, ethanol, isopropanol,
I will primarily be looking at fungi, bacteria and plant materials, with the occasional cheek swab
I do not want anything overly toxic/harmful as I have a toddler, these stains will be kept out of reach, but you can never be too careful.
I am OK with using water soluble or spirit soluble stains, low concentrations of acids are OK too.
anyways, if you can look at the list, and if you spot any "MUST HAVE stains" please let me know, I appreciate taking advantage of those with more experience than myself
Here is the list:
acid fucshin
acridine orange
acriflavine (neutral) acriflavine (trypaflavin)
acridingelb extra alcian Blue 8GS
alcian Blue 8GN (ingrain blue 1)
allizarin yellow GG amaranth red, USP (aniline red)
ammonium molybdate
aniline yellow (fast yellow S)
aniline blue BB
aniline blue
aniline blue aniline blue
aniline blue anthacene (blue violet fluorescence)
auramin O
auramin O
aurin
aurophosphin
azocarmine B
azocarmine G
azocoll substrate <50 mesh
azur A
azur A
azur I
azur II
basic fuchsin
blue dextran
brilliant dianilgrun
brilliant sulfaflavine
brom cresol green
brom phenol blue
benzopurpurin 48
blue tetrazolium
brazilin
brilliant cresyl blue
brilliant phosphin
brilliant sulfolfavin
brom cresol green
brom phenol blue
brom thymol blue
cacodylic acid (sodium salt)
calcofluor M2R enhancer
chelidonium extract
chlorophyll extract chrome azurol S
chromotrope 2R(fast fuchsin G) chrysarobin
Clayton yellow
congo red (direct red #28)
congo red congo red
congorot (congo red) coomassie brilliant blue G250
copper stain for electrophoresis
copper destain
corip
Do your worst!
I'm surprised it hasn't decade.
For context I'm a Refuse Driver (Garbage man) & today I was on food waste. After I'd tipped I was checking the wagon for any defects when I spotted a lone pea balanced on the lifts.
I said "hey look, an escaPEA"
No one near me but it didn't half make me laugh for a good hour or so!
Edit: I can't believe how much this has blown up. Thank you everyone I've had a blast reading through the replies 😂
It really does, I swear!
Because she wanted to see the task manager.
They’re on standbi
Pilot on me!!
Nothing, he was gladiator.
Heard they've been doing some shady business.
Dad jokes are supposed to be jokes you can tell a kid and they will understand it and find it funny.
This sub is mostly just NSFW puns now.
If it needs a NSFW tag it's not a dad joke. There should just be a NSFW puns subreddit for that.
Edit* I'm not replying any longer and turning off notifications but to all those that say "no one cares", there sure are a lot of you arguing about it. Maybe I'm wrong but you people don't need to be rude about it. If you really don't care, don't comment.
When I got home, they were still there.
What did 0 say to 8 ?
" Nice Belt "
So What did 3 say to 8 ?
" Hey, you two stop making out "
I won't be doing that today!
Where ever you left it 🤷♀️🤭
[Removed]
This morning, my 4 year old daughter.
Daughter: I'm hungry
Me: nerves building, smile widening
Me: Hi hungry, I'm dad.
She had no idea what was going on but I finally did it.
Thank you all for listening.
There hasn't been a post all year!
You take away their little brooms
It was about a weak back.
It’s pronounced “Noel.”
After all his first name is No-vac
What, then, is Chinese rap?
Edit:
Notable mentions from the comments:
-
Spanish/Swedish/Swiss/Serbian hits
-
French/Finnish art
-
Country/Canadian rap
-
Chinese/Country/Canadian rock
-
Turkish/Tunisian/Taiwanese rap
There hasn't been a single post this year!
(Happy 2022 from New Zealand)
