For an Intensity over m/z graph, when referring to 'base peak' does it refer to the highest peak on the graph or the peak with the highest intensity. I've literally had 'base peak' referred to as both definitions on separate exams when it asks with a graph so I'm hella confused. For reference, I'm looking at question 8ai) on the 2021 NHT Chem Exam, where I incorrectly stated that 57 was the base peak, when the answer was 41.
Any help would be much appreciated !
I already solved all possible structures for the other peaks but I don't know what could 18 possibly be other than water but the spectrometer wouldn't pick it up since water is neutral so what structure can this peak be? I concluded that the molecule analyzed is a carboxilic acid which containts bromine (C3H5O2Br)
https://preview.redd.it/1wh3tdcuthc71.png?width=716&format=png&auto=webp&s=d17e44c06a263a268466af3d5f028009fe1d2b33
What are the reasons to use Western Blotting instead of a mass-spec workflow to detect target proteins?
LC-MS has advantages of multiplexing, throughput, quantification, detection of PTMs and doesn't require antibodies specific to each protein being measured. So what are the downsides?
For my research I need to compare mass spectra I obtain with databases. So far I have used the NIST database but I'd like to know if there are other well known and/or free databases available. Cheers
Hey all, So you read the title. I'm in Orgo 2 now and tbh this was my weakest area from Orgo 1 (mainly because my orgo 1 class went over this during the switch to online and everything was a blur from then). Everytime I look at my Orgo textbook or at random google links my mind just gets clouded and increasingly confused at all these spectrometry & spectroscopy stuff and I'm lowkey starting to panic. Any advice? If you guys have any advice for Orgo 2 stuff I'm all ears too! PS: Thankyou guys in advance. I have been a lurker on this subreddit for quite a while & wouldn't have found motivation to get through Orgo 1 last spring if it weren't for you guys.
im reviewing how mass spec works and its just not clicking like when i took orgo 2. Do we need to know it realistically, and if so, how much do we need to know?
title. not expected a full structure, but looking for great tips. thanks!
I hope this isn't too dumb of a question.. we've just covered mass spectrometry and IR spectroscopy in chemistry so I was curious!
Had some help in ELI5 but can’t quite grasp it. Chem test coming up and I’d appreciate as much help as possible. Feel free to treat me like I don’t understand organic chemistry! (because I don’t)
Need to analyse the accuracy of MS/How does it compare to Atomic Absorption Spectroscopy?
Need an easy explanation without using difficult vocabularies...
Can someone help me understand how a decrease in m/z ratio (by increasing charge) causes a greater signal intensity?
Hey guys, long time lurker here. I see a lot of post where people ask if the blotter they have is legitimate or not, and always the first reply suggest getting a test kit. Well I personally advocate and preach harm reduction, but have never had the opportunity to do so myself, as my hook up is just straight into geltabs.
Well I recently got a half sheet of GDF WoW unperfed a5 art paper and don't have an ehrlich, but do have access to a mass spec. Just wanted to say that the results i got were fucking amazing! Enough to give any chemist a huge raging boner. Just wanted to point out that there is some amazing talent out there, and the best stuff usually isn't mass circulated. Peace, love and pyramids
Love you guys, stay safe.
