Hi! I am planning on performing bacterial growth curve measurements under anaerobic conditions. I will be using the GasPak system. What is a good protocol flow to use for these types of experiments? I will be measuring both OD600 and spot plating.
Thanks!
Hi, I want to activate a freeze-dried actinobacillus succinogenes under anaerobic conditions, to avoid the overexpression of LDH. The problem is that they recommend to activate it under aerobic conditions and don't talk about if it's possible to do the same under anaerobic conditions. Is there any problem if I try to do it in anaerobiosis ?
I’m worried about Capnocytophaga Canimorsus specifically.
Destroroyah has the micro-oxygen beam which is deadly to us because we are aerobic organisms that depend on the target of the micro-oxygen beam oxygen.
>Herodah is composed almost entirely of a mineral called Hedrium, an acidic, sludge-like material, which he uses as his primary means of attack. Hedorah can launch pieces of his body at opponents, which will burn or smother them. Hedorah can also discharge large quantities of sludge without impacting his size in any way, shown in Godzilla vs. Hedorah when he attempts to drown Godzilla in a pit by dumping a continuous stream of sludge onto him.
Like if it can use a non-oxygen terminal electron acceptor, does that mean it doesn't have to ferment?
Chloroflexus islandicus is a photosynthetic bacterium isolated from the Strokkur Geyser in Iceland. This organism is thermophilic showing optimal growth at 55°C (131°F) with a pH range of 7.5 – 7.7. C. islandicus grows best photoheterotrophically under anaerobic conditions with light but is capable of chemoheterotrophically growth under aerobic conditions in the dark. C. islandicus has a yellowish green color. The individual cells form unbranched multicellular filaments about 0.6 µm in diameter and 4-7 µm in length.
I know humans can't because our ATP needs are too high, but I was wondering if any organism is able to survive on anaerobic respiration. I'm guessing they'd be micro-organisms, and they'd probably have to be able to:
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Survive off only 2ATP per glucose and still somehow have energy to go get that glucose
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Have an efficient way of purging lactic acid (or whatever byproducts they have)
Just as the title says, could an organism the size of a cat, derived from microorganisms, efficiently survive using only anaerobic respiration? If so what adaptations would it need?
Maybe anaerobes of today can not get any bigger, complex and smarter than Ascaris because of the competition with aerobes, but what if the "Oxygen catastrophe" had never happened? Could anaerobe evolution produce big animals and, maybe, even into something smart?
If so, then there is a chance that "aliens" can be anaerobic, too, and hence the search for theoretically conscious life-applicable planets in the space should include atmospheres that are not oxygen-rich at all.
Thanks in advance.
I can't believe such a simple fact is so infuriating to Google. I search, and there's no straight answer. It's just "here's some examples of what can, and some things can if there's no oxygen". Reworded it a few times, no dice.
So I google which CAN'T do anaerobic respiration. And I get some answers that say "some cells can't do anaerobic respiration, so instead they do fermentation". But isn't fermentation what anaerobic respiration is? I was taught 2 types of anaerobic respiration, being lactic acid fermentation, and alcohol fermentation.
Let me get this out there first, why the hell must you guys torture potatoes who are trying not to make a fool of themselves like me? Okay, now I have done ranting, do you guys recommend doing La liaison facultative like in the phrase <jeux intéressants> or <Nous sommes allés>? I get that it is a regional and optional thing but in general, is it better to learn it in order to understand others and speak in a clearer fashion?
I got an anaerobic proccessed bag of beans and after reading about them it says that they go through fermentation so i was asking myself if they contained alcohol, thanks
So I did an early-bird racing clinic this weekend and it is causing me to question what I should target.
My issue isn't endurance, but it's being able to go back and forth, on and off, with the surging peloton.
I can put out steady power without much issue. But continually applying the watts to stay with the pack leaves me in what we used to refer to as oxygen debt. I'm unable to maintain deep breathing and end up short of breath, gasping. My HR doesn't register that it's spiking during this but it sure feels like it with the gasping.
I did have asthma as a kid and have just now started being treated for it as an adult with an inhaler and I see a little relief on training rides, but with racing situations, not so much.
Is this something to target with VO2 Max workouts or anaerobic workouts, intervals? Or are they both close enough that they will accomplish the same goal?
What would be the best metric test to evaluate improvement before hitting a race. I have about a month and a half before my first real race and opportunities for more practice races before that.
I'm just now moving back on to TrainerRoad for structured training.
Has anyone seen results from the breathing training tools like Airofit? After surgery a few years ago the doctor gave me a unit to practice breathing more fully. It made a ball rise in a tube and you could measure results. Of course I tossed it.
