Bacterial growth curves for anaerobic (facultative) microbe?

Hi! I am planning on performing bacterial growth curve measurements under anaerobic conditions. I will be using the GasPak system. What is a good protocol flow to use for these types of experiments? I will be measuring both OD600 and spot plating.

Thanks!

πŸ‘︎ 5
πŸ’¬︎
πŸ‘€︎ u/iineedacookie
πŸ“…︎ Oct 17 2021
🚨︎ report
Comparative Analysis of Gut Microbial Communities in Children under 5 Years Old with Diarrhea. proportion of aerobic and facultative anaerobic microbes in infectious group was much higher than the non-infectious group. relative abundance of Enterococcus significantly higher in healthy control group jmb.or.kr/journal/viewJou…
πŸ‘︎ 3
πŸ’¬︎
πŸ‘€︎ u/MaximilianKohler
πŸ“…︎ Apr 17 2018
🚨︎ report
Can I activate a freeze-dried facultative anaerobe under anaerobic conditions?

Hi, I want to activate a freeze-dried actinobacillus succinogenes under anaerobic conditions, to avoid the overexpression of LDH. The problem is that they recommend to activate it under aerobic conditions and don't talk about if it's possible to do the same under anaerobic conditions. Is there any problem if I try to do it in anaerobiosis ?

πŸ‘︎ 6
πŸ’¬︎
πŸ‘€︎ u/Lord_codfish
πŸ“…︎ Oct 01 2018
🚨︎ report
Specific, but does anyone know how long Facultative Anaerobic bacteria lasts on surfaces like floor carpets, leather, and tiles?

I’m worried about Capnocytophaga Canimorsus specifically.

πŸ‘︎ 3
πŸ’¬︎
πŸ‘€︎ u/konodioda879
πŸ“…︎ Apr 18 2020
🚨︎ report
Increased D-Lactic Acid Intestinal Bacteria in Patients with Chronic Fatigue Syndrome: his study suggests a probable link between intestinal colonization of Gram positive facultative anaerobic D-lactic acid bacteria and symptom expressions in a subgroup of patients with CFS. iv.iiarjournals.org/conte…
πŸ‘︎ 15
πŸ’¬︎
πŸ‘€︎ u/shallah
πŸ“…︎ Sep 23 2016
🚨︎ report
le anaerobic organism has arrived
πŸ‘︎ 693
πŸ’¬︎
πŸ‘€︎ u/YaBoiFulan
πŸ“…︎ May 30 2021
🚨︎ report
Increased D-Lactic Acid Intestinal Bacteria in Patients with Chronic Fatigue Syndrome: This study suggests a probable link between intestinal colonization of Gram positive facultative anaerobic D-lactic acid bacteria & symptom expressions in a subgroup of patients with CFS researchgate.net/profile/…
πŸ‘︎ 12
πŸ’¬︎
πŸ‘€︎ u/shallah
πŸ“…︎ Dec 30 2015
🚨︎ report
Increased D-Lactic Acid Intestinal Bacteria in Patients with Chronic Fatigue Syndrome: This study suggests a probable link between intestinal colonization of Gram positive facultative anaerobic D-lactic acid bacteria & symptom expressions in a subgroup of patients with CFS researchgate.net/profile/…
πŸ‘︎ 2
πŸ’¬︎
πŸ‘€︎ u/shallah
πŸ“…︎ Jan 01 2016
🚨︎ report
[WP] Humans have achieved interstellar travel and are seeding planets across the galaxy with photosynthetic organisms to make the planets suitable for human colonies. The anaerobic life that live on these worlds don't take this act of attempted genocide lightly
πŸ‘︎ 19
πŸ’¬︎
πŸ“…︎ May 17 2021
🚨︎ report
Symbiosis can be obligate, where two organisms cannot survive without each other or facultative, where species live together by choice. They vary between mutualism (positive for both), commensalism (positive for 1, neutral for the other), and parasitism (positive for 1, negative for the other). en.wikipedia.org/wiki/Sym…
πŸ‘︎ 81
πŸ’¬︎
πŸ‘€︎ u/FillsYourNiche
πŸ“…︎ Jan 29 2017
🚨︎ report
checkmate anaerobic organisms
πŸ‘︎ 233
πŸ’¬︎
πŸ‘€︎ u/nonnoxer
πŸ“…︎ Nov 08 2019
🚨︎ report
Who would win between the only 2 anaerobic hive organism monsters? Destoroyah vs Hedorah

Destroroyah has the micro-oxygen beam which is deadly to us because we are aerobic organisms that depend on the target of the micro-oxygen beam oxygen.

>Herodah is composed almost entirely of a mineral called Hedrium, an acidic, sludge-like material, which he uses as his primary means of attack. Hedorah can launch pieces of his body at opponents, which will burn or smother them. Hedorah can also discharge large quantities of sludge without impacting his size in any way, shown in Godzilla vs. Hedorah when he attempts to drown Godzilla in a pit by dumping a continuous stream of sludge onto him.

-Wikizilla

πŸ‘︎ 11
πŸ’¬︎
πŸ‘€︎ u/Non-profitboi
πŸ“…︎ Sep 12 2019
🚨︎ report
Does fermentation occur in all anaerobic organisms?

Like if it can use a non-oxygen terminal electron acceptor, does that mean it doesn't have to ferment?

πŸ‘︎ 3
πŸ’¬︎
πŸ‘€︎ u/JoeyBobBillie
πŸ“…︎ Apr 11 2020
🚨︎ report
First time seeing this organism. Anaerobic Blood culture bottle.
πŸ‘︎ 11
πŸ’¬︎
πŸ‘€︎ u/Labrechaun
πŸ“…︎ Nov 18 2018
🚨︎ report
[MEME] im a facultative anaerobe
πŸ‘︎ 120
πŸ’¬︎
πŸ‘€︎ u/deezpantshit
πŸ“…︎ Feb 19 2019
🚨︎ report
Chloroflexus islandicus is a photosynthetic bacterium isolated from the Strokkur Geyser in Iceland. This organism is thermophilic showing optimal growth at 55Β°C (131Β°F) with a pH range of 7.5 – 7.7. C. islandicus grows best photoheterotrophically under anaerobic conditions with light but is capable

Chloroflexus islandicus is a photosynthetic bacterium isolated from the Strokkur Geyser in Iceland. This organism is thermophilic showing optimal growth at 55Β°C (131Β°F) with a pH range of 7.5 – 7.7. C. islandicus grows best photoheterotrophically under anaerobic conditions with light but is capable of chemoheterotrophically growth under aerobic conditions in the dark. C. islandicus has a yellowish green color. The individual cells form unbranched multicellular filaments about 0.6 Β΅m in diameter and 4-7 Β΅m in length.

πŸ‘︎ 2
πŸ’¬︎
πŸ‘€︎ u/KnightOfDoom22
πŸ“…︎ Oct 16 2019
🚨︎ report
Is there any organism which can survive long term on anaerobic respiration alone?

I know humans can't because our ATP needs are too high, but I was wondering if any organism is able to survive on anaerobic respiration. I'm guessing they'd be micro-organisms, and they'd probably have to be able to:

  • Survive off only 2ATP per glucose and still somehow have energy to go get that glucose

  • Have an efficient way of purging lactic acid (or whatever byproducts they have)

πŸ‘︎ 5
πŸ’¬︎
πŸ‘€︎ u/JoseDzirehChong
πŸ“…︎ Mar 27 2019
🚨︎ report
Would it be possible to put anaerobic organisms on a planet to help make it habitable for a larger human population?
πŸ‘︎ 9
πŸ’¬︎
πŸ‘€︎ u/dtamura18
πŸ“…︎ May 15 2018
🚨︎ report
Could an organism survive with anaerobic respiration?

Just as the title says, could an organism the size of a cat, derived from microorganisms, efficiently survive using only anaerobic respiration? If so what adaptations would it need?

πŸ‘︎ 2
πŸ’¬︎
πŸ‘€︎ u/MagentaDinoNerd
πŸ“…︎ Sep 22 2018
🚨︎ report
TIL that many famous pathogens, such as Salmonella, E. Coli, and Y. Pestis, are all Facultative Anaerobes, that is to say, they prefer to "breathe" oxygen, but can switch to fermentation in its absence en.wikipedia.org/wiki/Fac…
πŸ‘︎ 74
πŸ’¬︎
πŸ‘€︎ u/Jozarin
πŸ“…︎ Sep 16 2019
🚨︎ report
Why anaerobic organisms can not grow much bigger than, say, Ascaris? Are anaerobes able to evolve into a conscious life?

Maybe anaerobes of today can not get any bigger, complex and smarter than Ascaris because of the competition with aerobes, but what if the "Oxygen catastrophe" had never happened? Could anaerobe evolution produce big animals and, maybe, even into something smart?

If so, then there is a chance that "aliens" can be anaerobic, too, and hence the search for theoretically conscious life-applicable planets in the space should include atmospheres that are not oxygen-rich at all.

Thanks in advance.

πŸ‘︎ 9
πŸ’¬︎
πŸ‘€︎ u/RRDDSS
πŸ“…︎ Apr 29 2017
🚨︎ report
[Hig School Biology] Which organisms perform anaerobic respiration?

I can't believe such a simple fact is so infuriating to Google. I search, and there's no straight answer. It's just "here's some examples of what can, and some things can if there's no oxygen". Reworded it a few times, no dice.

So I google which CAN'T do anaerobic respiration. And I get some answers that say "some cells can't do anaerobic respiration, so instead they do fermentation". But isn't fermentation what anaerobic respiration is? I was taught 2 types of anaerobic respiration, being lactic acid fermentation, and alcohol fermentation.

πŸ‘︎ 2
πŸ’¬︎
πŸ‘€︎ u/SYZekrom
πŸ“…︎ Apr 02 2017
🚨︎ report
TIL that in many anaerobic organisms, including some animals, the hydrogenosome is the powerhouse of the cell en.wikipedia.org/wiki/Hyd…
πŸ‘︎ 13
πŸ’¬︎
πŸ‘€︎ u/AOEUD
πŸ“…︎ Nov 09 2015
🚨︎ report
Genomic Expansion of Domain Archaea Highlights Roles for Organisms from New Phyla in Anaerobic Carbon Cycling cell.com/current-biology/…
πŸ‘︎ 6
πŸ’¬︎
πŸ‘€︎ u/burtzev
πŸ“…︎ Mar 16 2015
🚨︎ report
Genomic Expansion of Domain Archaea Highlights Roles for Organisms from New Phyla in Anaerobic Carbon Cycling cell.com/current-biology/…
πŸ‘︎ 3
πŸ’¬︎
πŸ‘€︎ u/burtzev
πŸ“…︎ Mar 16 2015
🚨︎ report
Twelve egg-shaped anaerobic digesters on Deer Island break down our waste into methane gas, carbon dioxide, solid organic byproducts, and water (second largest sewage treatment plant in the U.S.)
πŸ‘︎ 202
πŸ’¬︎
πŸ‘€︎ u/alanboston
πŸ“…︎ Jul 05 2021
🚨︎ report
The first multi-cellular, anaerobic organism known to humans boingboing.net/2010/04/07…
πŸ‘︎ 13
πŸ’¬︎
πŸ‘€︎ u/DrJulianBashir
πŸ“…︎ Apr 08 2010
🚨︎ report
Incredibly preserved 2400 year old shipwreck compared to a contemporary depiction. It was found off the coast of Bulgaria. Bellow the Black Sea 200 meters /650feet it's completely anaerobic which prevents organic material from deterioration. It's also complete darkness, the image is photogrammetry.
πŸ‘︎ 90
πŸ’¬︎
πŸ‘€︎ u/r3vange
πŸ“…︎ Jun 01 2021
🚨︎ report
"Facultative Parthenogenesis in California Condors", Ryder et al 2021 academic.oup.com/jhered/a…
πŸ‘︎ 13
πŸ’¬︎
πŸ‘€︎ u/gwern
πŸ“…︎ Oct 28 2021
🚨︎ report
Classic example of Armillaria and how it is a facultative parasite
πŸ‘︎ 350
πŸ’¬︎
πŸ“…︎ Oct 16 2021
🚨︎ report
New paper studying cut marks on 1.9 million year old animal bones finds evidence of primary usage by hominids, meaning that humans were hunting and butchering animals themselves, not just stealing other carnivores' kills. Discussion makes strong case for facultative & obligate carnivory in humans. /r/ketoscience/comments/p…
πŸ‘︎ 53
πŸ’¬︎
πŸ‘€︎ u/dem0n0cracy
πŸ“…︎ Aug 10 2021
🚨︎ report
La liaison facultative, should I do it?

Let me get this out there first, why the hell must you guys torture potatoes who are trying not to make a fool of themselves like me? Okay, now I have done ranting, do you guys recommend doing La liaison facultative like in the phrase <jeux intΓ©ressants> or <Nous sommes allΓ©s>? I get that it is a regional and optional thing but in general, is it better to learn it in order to understand others and speak in a clearer fashion?

πŸ‘︎ 6
πŸ’¬︎
πŸ“…︎ Nov 06 2021
🚨︎ report
Do anaerobic proccessed bean have alcohol?

I got an anaerobic proccessed bag of beans and after reading about them it says that they go through fermentation so i was asking myself if they contained alcohol, thanks

πŸ‘︎ 77
πŸ’¬︎
πŸ“…︎ Dec 23 2021
🚨︎ report
Anyone know of any roasters who offer the same coffee, roasted with different processes? Would love to try the same coffee as a washed vs honey vs anaerobic, etc. thanks !
πŸ‘︎ 47
πŸ’¬︎
πŸ‘€︎ u/Lcphilly
πŸ“…︎ Jan 05 2022
🚨︎ report
Would I target anaerobic or VO2 max?

So I did an early-bird racing clinic this weekend and it is causing me to question what I should target.

My issue isn't endurance, but it's being able to go back and forth, on and off, with the surging peloton.

I can put out steady power without much issue. But continually applying the watts to stay with the pack leaves me in what we used to refer to as oxygen debt. I'm unable to maintain deep breathing and end up short of breath, gasping. My HR doesn't register that it's spiking during this but it sure feels like it with the gasping.

I did have asthma as a kid and have just now started being treated for it as an adult with an inhaler and I see a little relief on training rides, but with racing situations, not so much.

Is this something to target with VO2 Max workouts or anaerobic workouts, intervals? Or are they both close enough that they will accomplish the same goal?

What would be the best metric test to evaluate improvement before hitting a race. I have about a month and a half before my first real race and opportunities for more practice races before that.

I'm just now moving back on to TrainerRoad for structured training.

Has anyone seen results from the breathing training tools like Airofit? After surgery a few years ago the doctor gave me a unit to practice breathing more fully. It made a ball rise in a tube and you could measure results. Of course I tossed it.

πŸ‘︎ 15
πŸ’¬︎
πŸ‘€︎ u/DougalisGod
πŸ“…︎ Jan 10 2022
🚨︎ report
Would it be possible to put anaerobic organisms on a planet to help make it habitable for a larger human population?
πŸ‘︎ 4
πŸ’¬︎
πŸ‘€︎ u/dtamura18
πŸ“…︎ May 16 2018
🚨︎ report
are there any anaerobic multicellular organisms?
πŸ‘︎ 5
πŸ’¬︎
πŸ‘€︎ u/wanabeswordsman
πŸ“…︎ Aug 24 2014
🚨︎ report

Please note that this site uses cookies to personalise content and adverts, to provide social media features, and to analyse web traffic. Click here for more information.