My latest bloodwork showed that in addition to Positive ANA with Nuclear homogenous, I have Mitotic- Intercellular Bridge Staining A-27. I've been trying to find info on it but what's out there is a lot of medical legalese and I haven't found anyone else who says they've had this.
I messaged my Rheum but didn't get an answer.
Anyone have experience with this and if so, what did it indicate for you?
This is my hail Mary pass. Please labrats, save me from wasting a month trying a million different antibodies!
I have one finicky antibody that looks best following a 10 minute fix in ice cold acetone/methanol, but it's so expensive to buy and try antibodies only to find out they aren't compatible with this fixation.
Mechanical interactions between cells have been shown to play critical roles in regulating cell signaling and communications. However, the precise measurement of intercellular forces is still quite challenging, especially considering the complex environment at cell–cell junctions. In this study, we report a fluorescence lifetime‐based approach to image and quantify intercellular molecular tensions. Using this method, tensile forces among multiple ligand‐receptor pairs can be measured simultaneously. We first validated our approach and developed lifetime measurement‐based DNA tension probes to image E‐cadherin‐mediated tension on epithelial cells. These probes were then further applied to quantify the correlations between E‐cadherin and N‐cadherin tensions during an epithelial‐mesenchymal transition process. The modular design of these probes can potentially be used to study the mechanical features of various physiological and pathological processes.
https://ift.tt/3vXkuwV
https://doi.org/10.1016/j.cmet.2020.11.008
https://pubmed.ncbi.nlm.nih.gov/33278339
Abstract
Recent studies suggest that mitochondria can be transferred between cells to support the survival of metabolically compromised cells. However, whether intercellular mitochondria transfer occurs in white adipose tissue (WAT) or regulates metabolic homeostasis in vivo remains unknown. We found that macrophages acquire mitochondria from neighboring adipocytes in vivo and that this process defines a transcriptionally distinct macrophage subpopulation. A genome-wide CRISPR-Cas9 knockout screen revealed that mitochondria uptake depends on heparan sulfates (HS). High-fat diet (HFD)-induced obese mice exhibit lower HS levels on WAT macrophages and decreased intercellular mitochondria transfer from adipocytes to macrophages. Deletion of the HS biosynthetic gene Ext1 in myeloid cells decreases mitochondria uptake by WAT macrophages, increases WAT mass, lowers energy expenditure, and exacerbates HFD-induced obesity in vivo. Collectively, this study suggests that adipocytes and macrophages employ intercellular mitochondria transfer as a mechanism of immunometabolic crosstalk that regulates metabolic homeostasis and is impaired in obesity.
------------------------------------------ Info ------------------------------------------
Open Access: False
Authors: Jonathan R. Brestoff - Craig B. Wilen - John R. Moley - Yongjia Li - Wei Zou - Nicole P. Malvin - Marina N. Rowen - Brian T. Saunders - Hongming Ma - Madison R. Mack - Barry L. Hykes - Dale R. Balce - Anthony Orvedahl - Jesse W. Williams - Nidhi Rohatgi - Xiaoyan Wang - Michael R. McAllaster - Scott A. Handley - Brian S. Kim - John G. Doench - Bernd H. Zinselmeyer - Michael S. Diamond - Herbert W. Virgin - Andrew E. Gelman - Steven L. Teitelbaum -
Additional links: None found
One day, Jeff Winger was at his desk feeling sad, staring at his phone. When he looked up, he saw Elroy at the door. With a big grin, Elroy said, "Now there's a man who knows how to use a cellphone." Jeff Winger smiled.
A fluorescence lifetime imaging-based cell membrane-anchored DNA hairpin probe is designed to measure tensile forces at cell–cell junctions. The tension among multiple ligand–receptor pairs can be quantified simultaneously during cellular processes such as the epithelial–mesenchymal transition.
Abstract
Mechanical interactions between cells have been shown to play critical roles in regulating cell signaling and communications. However, the precise measurement of intercellular forces is still quite challenging, especially considering the complex environment at cell–cell junctions. In this study, we report a fluorescence lifetime-based approach to image and quantify intercellular molecular tensions. Using this method, tensile forces among multiple ligand–receptor pairs can be measured simultaneously. We first validated our approach and developed lifetime measurement-based DNA tension probes to image E-cadherin-mediated tension on epithelial cells. These probes were then further applied to quantify the correlations between E-cadherin and N-cadherin tensions during an epithelial–mesenchymal transition process. The modular design of these probes can potentially be used to study the mechanical features of various physiological and pathological processes.
https://ift.tt/3vXkuwV
