Question ; I have a model that predicts a cis-regulatory element - which protocol/experiment let's me tag it to see if proteins interact with it? and if possible identify which proteins?

My molecular techniques is rusty. Been years since I've read Gene Cloning and DNA Analysis by TA Brown; might pick up the latest version.

A bait/prey setup comes to mind, but would be difficult to do for many proteins... hope there are better approaches

Thx!

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πŸ‘€︎ u/paswut
πŸ“…︎ Dec 26 2021
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Quercetin impairs learning and memory in normal mice via suppression of hippocampal phosphorylated cyclic AMP response element-binding protein expression

https://pubmed.ncbi.nlm.nih.gov/20466049/

Quercetin is a naturally occurring dietary flavonol and several reports have shown that quercetin substantially affects cognitive function in disease models, which suggests that quercetin might be a useful agent for treatment of memory dysfunction. However, only one report has examined the effects of quercetin on normal cognitive function. In the present study, we investigated the potential deleterious effects of quercetin on normal cognitive function using Western blot assays and the following behavioral tasks: passive avoidance, Y-maze, and Morris water maze. In the passive avoidance task, pre-acquisition administration of quercetin (10, 20, or 40 mg/kg, p.o.) caused significant cognitive impairments in mice (P < 0.05 or P < 0.01). Quercetin-treated groups (10, 20, or 40 mg/kg, p.o.) also showed significant memory impairments compared with the control group in the Y-maze task (P < 0.05). In the Morris water maze task, there were no significant differences among the groups during training trial sessions, but at the probe trial session, the quercetin-treated group (40 mg/kg, p.o.) spent significantly less time in the target quadrant than did the control group (P < 0.05). In Western blot assays of hippocampal tissue, we found that quercetin-treated groups showed decreased expression of phosphorylated Akt (pAkt), phosphorylated calcium-calmodulin kinase II (pCaMKII), and phosphorylated cyclic AMP response element-binding protein (pCREB). These results suggest that acute administration of quercetin impairs cognitive function by suppression of pAkt and pCaMKII, which, in turn, decreases pCREB expression in the hippocampus.

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πŸ‘€︎ u/BoostYourBiology
πŸ“…︎ Jan 12 2022
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Quercetin impairs learning and memory in normal mice via suppression of hippocampal phosphorylated cyclic AMP response element-binding protein expression (2010) pubmed.ncbi.nlm.nih.gov/2…
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πŸ“…︎ Apr 16 2021
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Can protein phosphatases be considered regulatory elements in their own right, or are they just 'reset switches' for kinases?
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πŸ“…︎ Dec 24 2020
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Lanmodulin protein, which binds rare-earth elements (15 lanthanides, along with scandium and yttrium) can effectively extract and separate these valuable metals from low-grade sources, such as coal ash or electronic waste archive.md/UQecN
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πŸ‘€︎ u/snooshoe
πŸ“…︎ Oct 27 2021
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Lanmodulin protein, which binds rare-earth elements (15 lanthanides, along with scandium and yttrium) can effectively extract and separate these valuable metals from low-grade sources, such as coal ash or electronic waste archive.md/UQecN
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πŸ‘€︎ u/snooshoe
πŸ“…︎ Oct 27 2021
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Natural protein captures rare-earth elements better than synthetic chelators. A lanthanide-binding protein found in bacteria could extract rare-earth metals more efficiently and with less waste. cen.acs.org/analytical-ch…
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πŸ‘€︎ u/MistWeaver80
πŸ“…︎ Aug 31 2020
🚨︎ report
Lanmodulin protein, which binds rare-earth elements (15 lanthanides, along with scandium and yttrium) can effectively extract and separate these valuable metals from low-grade sources, such as coal ash or electronic waste archive.md/UQecN
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πŸ‘€︎ u/snooshoe
πŸ“…︎ Oct 27 2021
🚨︎ report
Lanmodulin protein, which binds rare-earth elements (15 lanthanides, along with scandium and yttrium) can effectively extract and separate these valuable metals from low-grade sources, such as coal ash or electronic waste archive.md/UQecN
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πŸ‘€︎ u/snooshoe
πŸ“…︎ Oct 27 2021
🚨︎ report
Lanmodulin protein, which binds rare-earth elements (15 lanthanides, along with scandium and yttrium) can effectively extract and separate these valuable metals from low-grade sources, such as coal ash or electronic waste archive.md/UQecN
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πŸ‘€︎ u/snooshoe
πŸ“…︎ Oct 27 2021
🚨︎ report
Lanmodulin protein, which binds rare-earth elements (15 lanthanides, along with scandium and yttrium) can effectively extract and separate these valuable metals from low-grade sources, such as coal ash or electronic waste archive.md/UQecN
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πŸ‘€︎ u/snooshoe
πŸ“…︎ Oct 27 2021
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mycobateria? which tools to find info? (conservation, phylogeny, celullar location of protein, regulatory elements in region)
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πŸ‘€︎ u/wizofe
πŸ“…︎ Jan 12 2018
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What does it mean if cAMP response element-binding protein is upregulated? What is the result?

So I ask this question because I am doing some research, and I am trying to understand this article: https://www.thieme-connect.com/products/ejournals/pdf/10.1055/s-0035-1545937.pdf

So what exactly is cAMP? And what's a response element-bind protein? I did do some research online, but I still don't feel as If I fully understand.

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πŸ‘€︎ u/TheReviewNinja
πŸ“…︎ Jun 28 2017
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Concomitant expression of far upstream element (FUSE) binding protein (FBP) interacting repressor (FIR) and its splice variants induce migration and invasion of non-small cell lung cancer (NSCLC) cells onlinelibrary.wiley.com/d…
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πŸ‘€︎ u/montaukwhaler
πŸ“…︎ Jul 18 2015
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