I've been suffering from deliberating digestive issues for a couple of years, since a food poisoning incident in Cambodia. My current hypothesis is, that some Klebsiella species is causing my problems (a large population of Klebsiella has been confirmed by testing). I'm currently on a carnivore diet in a desperate attempt to "starve" Klebsiella. I feel fine while on this diet, but assume that I might relapse as soon as I add something else to my intake. I thinking about having a glass of whiskey with some friends tomorrow, but don't want to fuck things up, so I'm wondering if high percentage alcohol may feed Klebsiella, or if I can treat myself to it? :) Thanks
So I suffered from a longstanding mycoplasma infection which I thought I finally cleared (tested negative 4x, of which the latest was 8 weeks post antibiotics). However, at the hospital they have also done a culture and microscopy analysis of my urine.
Culture came back negative for microorganisms but microscopy (1000x magnification on oil immersion) showed gram negative bacteria score of 1-5 (I'm not sure what the reference value is). So this suggests I still have some sort of bacteria (that can't be cultured) but given the fact that i.had 4 consecutive negative PCRs I highly doubt it's mgen....
What's your take on this guys???
EDIT: I spoke lengthy with my doctor and she said there was nothing to worry about. The observed bacteria were non pathogenic and not growing. She said my symptoms have to be due to residual symptoms.
Journal of the American Chemical SocietyDOI: 10.1021/jacs.0c11113
Vadim Baidin, Tristan W. Owens, Michael B. Lazarus, and Daniel Kahne
https://ift.tt/3pZxuPv
I was reading about gram-negative bacteria and one (helicobacter pylori) was mentioned to be treated with amoxicillin.
I learnt that penicillin isnt effective against gram-negative bacteria because of the outer membrane, and amoxicillin being a beta-lactam would have to get through the outer membrane as well to have any effect, right?
So why can penicillin not get through the OM but amoxicillin can?
Thank you!!!
In other words, are gram negatives and gram positives two separate evolutionary groups? If so, was their common ancestor negative or positive? If not, does this mean that the peptidoglycan / lipopolysaccharide capsules evolved multiple times independently? I can't seem to find a clear answer for this.
Title pretty much says it all. I know that the endotoxin does have an effect in humans, however would the same be true for nearby gram positives?
Thanks!
The glycosylated cationic β‐peptide PAS8‐b‐PDM12 sensitizes the drug‐resistant ESKAPE Gram‐negative bacteria to multiple antibiotics by facilitating penetration through the outer membrane, and by deactivating efflux pump systems, opening an avenue for novel combination therapies for life‐threatening bacterial infections.
Abstract
Carbapenem‐resistant Gram‐negative bacteria (GNB) are heading the list of pathogens for which antibiotics are the most critically needed. Many antibiotics are either unable to penetrate the outer‐membrane or are excluded by efflux mechanisms. Here, we report a cationic block β‐peptide (PAS8‐b‐PDM12) that reverses intrinsic antibiotic resistance in GNB by two distinct mechanisms of action. PAS8‐b‐PDM12 does not only compromise the integrity of the bacterial outer‐membrane, it also deactivates efflux pump systems by dissipating the transmembrane electrochemical potential. As a result, PAS8‐b‐PDM12 sensitizes carbapenem‐ and colistin‐resistant GNB to multiple antibiotics in vitro and in vivo. The β‐peptide allows the perfect alternation of cationic versus hydrophobic side chains, representing a significant improvement over previous antimicrobial α‐peptides sensitizing agents. Together, our results indicate that it is technically possible for a single adjuvant to reverse innate antibiotic resistance in all pathogenic GNB of the ESKAPE group, including those resistant to last resort antibiotics.
https://ift.tt/2GZkIfb
Multi‐drug resistance in Gram‐negative bacteria is often associated with low permeability of the outer membrane. To investigate the role of membrane channels in the uptake of antibiotics here, we show a robust approach using fusion of native outer membrane vesicles (OMV) into planar lipid bilayer, which moreover allows also characterization of membrane protein channels in their native environment. Two major membrane channels from E. coli , OmpF and OmpC, were overexpressed from the host and the corresponding OMVs were collected. Each OMV fusion revealed surprisingly single or only a few channel activities. The asymmetry of the OMV´s translates after fusion into the lipid membrane with the LPS dominantly present at the side of OMV addition. Compared to conventional reconstitution method, the channels fused from OMVs containing LPS have similar conductance but a much broader distribution and significantly lower permeation. We suggest using outer membrane vesicles as a fast and easy approach for functional and structural studies of membrane channels in the native membrane.
https://ift.tt/2H05Cq0
The glycosylated cationic β‐peptide PAS8‐b‐PDM12 sensitizes the drug‐resistant ESKAPE Gram‐negative bacteria to multiple antibiotics by facilitating penetration through the outer membrane, and by deactivating efflux pump systems, opening an avenue for novel combination therapies for life‐threatening bacterial infections.
Abstract
Carbapenem‐resistant Gram‐negative bacteria (GNB) are heading the list of pathogens for which antibiotics are the most critically needed. Many antibiotics are either unable to penetrate the outer‐membrane or are excluded by efflux mechanisms. Here, we report a cationic block β‐peptide (PAS8‐b‐PDM12) that reverses intrinsic antibiotic resistance in GNB by two distinct mechanisms of action. PAS8‐b‐PDM12 does not only compromise the integrity of the bacterial outer‐membrane, it also deactivates efflux pump systems by dissipating the transmembrane electrochemical potential. As a result, PAS8‐b‐PDM12 sensitizes carbapenem‐ and colistin‐resistant GNB to multiple antibiotics in vitro and in vivo. The β‐peptide allows the perfect alternation of cationic versus hydrophobic side chains, representing a significant improvement over previous antimicrobial α‐peptides sensitizing agents. Together, our results indicate that it is technically possible for a single adjuvant to reverse innate antibiotic resistance in all pathogenic GNB of the ESKAPE group, including those resistant to last resort antibiotics.
https://ift.tt/2GZkIfb
