Stress granule Puns

Arsenite-induced stress granules isolated from both non-cancer and cancer cells contain reactive oxygen species as revealed by amperometric measurements with Pt or platinized carbon microelectrodes. This finding supports a role for SGs as signaling hubs and unveils a correlation between SG biology and pathogenesis in neurodegenerative diseases and some cancers.

Abstract

Stress granules (SGs) are membrane-less organelles that assemble in the cytoplasm to organize cellular contents and promote rapid adaptation during stress. To understand how SGs contribute to physiological functions, we used electrochemical measurements to detect electroactive species in SGs. With amperometry, we discovered that reactive oxygen species (ROS) are encapsulated inside arsenite-induced SGs, and H2O2 is the main species. The release kinetics of H2O2 from single SGs and the number of H2O2 molecules were quantified. The discovery that SGs contain ROS implicates them as communicators of the cellular stresses rather than a simple endpoint. This may explain how SGs regulate cellular metabolism and stress responses. This may also help better understand their cytoprotective functions in pathological conditions associated with SGs such as neurodegenerative diseases (NDs), cancers and viral infections.

https://ift.tt/3cvNUdU

Hi everyone!

I apologize if this isn't the perfect place to pose my question, I was looking through different subreddits and this just felt like the best bet I had to potentially ask a question and not get it deleted.

I am trying to find a software to download (preferably free) that I can use to quantify images I have from confocal microscopy of stress granules. I have used ImageJ in the past but am hoping someone has a different resource or potentially a trick they use to have ImageJ count the stress granules rather than me needing to click on them and count with my own two eyes.

Thank you so much!

Stress granules (SGs) are membrane‐less organelles that assemble in the cytoplasm to organize cellular contents and promote rapid adaptation during stress. To understand how SGs contribute to physiological functions, we used electrochemical measurements to detect electroactive species in SGs. With amperometry, we discovered that reactive oxygen species (ROS) are encapsulated inside arsenite‐induced SGs, and H 2 O 2 is the main species. The release kinetics of H 2 O 2 from single SGs and the number of H 2 O 2 molecules were quantified. The discovery that SGs contain ROS implicates them as communicators of the cellular stresses rather than a simple endpoint. This may explain how SGs regulate cellular metabolism and stress responses. This may also help better understand their cytoprotective functions in pathological conditions associated with SGs such as neurodegenerative diseases (NDs), cancers and viral infections.

https://ift.tt/3stCpck