Expression of Recombinant Green Fluorescent Protein (rGFP)

Expression and Purification of recombinant Green Fluorescent Protein (rGFP) from E. coli using Ni2+-Agarose Column Chromatography. Andrea Bustamante Janakikeerthika Darmarpandi Β  Green Fl.

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πŸ‘€︎ u/BananaOk8250
πŸ“…︎ Jan 05 2022
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Green fluorescent protein
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πŸ‘€︎ u/Tnemirepxe
πŸ“…︎ Nov 07 2021
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Are GFP(green fluorescent protein) Axolotls Rare?
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πŸ‘€︎ u/kierlanspicer
πŸ“…︎ Aug 31 2021
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[Article] Towards a Digital Twin: A validated Digital Model of a Pilot Scale Bioreactor producing Green Fluorescent Protein (GFP)

Title: Towards a Digital Twin: A validated Digital Model of a Pilot Scale Bioreactor producing Green Fluorescent Protein (GFP)

Journal: Computer Aided Chemical Engineering

DOI: 10.1016/B978-0-323-88506-5.50099-1

Link: https://www.sciencedirect.com/science/article/abs/pii/B9780323885065500991#!

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πŸ“…︎ Oct 09 2021
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Synthesis of Green Fluorescent Protein (GFP) Chromophore Analogues. Im a Second year university chemist. I’ve been a lurker for a while so I thought I would post some analytical chemistry synthesis. reddit.com/gallery/o0a5cs
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πŸ‘€︎ u/lmnop120
πŸ“…︎ Jun 15 2021
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TIL about glow-in-the-dark kitties. Scientists insert the GFP(green fluorescent protein from jellyfish) gene along with another gene that blocks feline AIDS, into the cat's unfertilized eggs. When the eggs are fertilized, glowing cats are produced, which means that they also have the anti-AIDS gene. smithsonianmag.com/scienc…
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πŸ‘€︎ u/girlamethyst
πŸ“…︎ Mar 06 2020
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TIL Alba was a genetically modified "glowing" rabbit created by artist Eduardo Kac. In daylight, Alba looks like a normal albino rabbit. But each of her cells contains the gene for a fluorescent protein taken from the jellyfish Aequorea victoria. In UV light, her body glows bright green. newscientist.com/article/…
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πŸ‘€︎ u/Freudwentcrazy
πŸ“…︎ Jan 04 2021
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[ASAP] Disentangling Chromophore States in a Reversibly Switchable Green Fluorescent Protein: Mechanistic Insights from NMR Spectroscopy

Journal of the American Chemical SocietyDOI: 10.1021/jacs.1c02442

Nina Eleni Christou, Karine Giandoreggio-Barranco, Isabel Ayala, Oleksandr Glushonkov, Virgile Adam, Dominique Bourgeois, and Bernhard Brutscher

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πŸ‘€︎ u/TomisMeMyselfandI
πŸ“…︎ May 10 2021
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Wanted to show y’all Noodle under blue light. He has the GFP (Green Fluorescent Protein) gene which gives him that yellow glow. Such a cutie! reddit.com/gallery/j5e2ro
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πŸ‘€︎ u/Hashermoney
πŸ“…︎ Oct 05 2020
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Developing zebrafish embryo genetically modified to express green fluorescent protein in the vasculature v.redd.it/dweqiqxsl8x01
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πŸ‘€︎ u/Afferent_Input
πŸ“…︎ May 11 2018
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Insulin and Green fluorescent protein with watercolor reddit.com/gallery/j97z5m
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πŸ‘€︎ u/phosphorylated
πŸ“…︎ Oct 11 2020
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Purified Enhanced Green Fluorescent Protein - It was soooo green.
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πŸ‘€︎ u/mujeresliebres
πŸ“…︎ Oct 09 2018
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A transgenic plant embryo at the "heart" stage inside its seed, expressing green fluorescent protein. I did it but unfortunately it was useless for me other than looking cool
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πŸ‘€︎ u/meanSh1t
πŸ“…︎ Sep 23 2020
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Just did a little experiment and infused a GFP (Green fluorescent protein) gene into E.Coli
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πŸ‘€︎ u/Getreddddd
πŸ“…︎ Jun 18 2020
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Green fluorescent protein, after a lot of support removal, printed in Ender5 with Cura
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πŸ‘€︎ u/memomemito
πŸ“…︎ Apr 27 2020
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Cross section of an adult human bicep. Fluorescent tags mark proteins that are responsible for quick contraction(gold) or slow contraction(green). Muscle fibers are generally either 'fast-twitch' for quick bursts, or 'slow-twitch' for endurance.
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πŸ‘€︎ u/XiphiasZ
πŸ“…︎ Jul 22 2018
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This is Aequorea victoria, a bioluminescent jellyfish. They’re incredibly important to science as they make a fluorescent green protein that lets biologists study gene expression
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πŸ‘€︎ u/the_karma_llama
πŸ“…︎ Sep 04 2019
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[todayilearned] TIL about glow-in-the-dark kitties. Scientists insert the GFP(green fluorescent protein from jellyfish) gene along with another gene that blocks feline AIDS, into the cat's unfertilized eggs. When the eggs are fertilized, glowing cats are produced, which means that they also have the smithsonianmag.com/scienc…
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πŸ‘€︎ u/Know_Your_Shit_v2
πŸ“…︎ Mar 06 2020
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Fluorescent Protein Containing Pendant w/ Glow in The Dark Structure reddit.com/gallery/rmeqs1
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πŸ‘€︎ u/Natolx
πŸ“…︎ Dec 22 2021
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Douglas Prasher, an American molecular biologist who was acknowledged by all three 2008 Chemistry Nobel laureates in their acceptance speeches for his work on the green fluorescent protein, couldn't find a job in science and was working as a bus driver for $8.50/h en.wikipedia.org/wiki/Dou…
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πŸ‘€︎ u/anarchisto
πŸ“…︎ Sep 08 2017
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TIL about Osamu Shimomura, Nobel laureate and National Academy of Sciences member, who survived the Nagasaki atomic bomb blast and went on to discover green fluorescent protein, fundamentally advancing basic biological research en.wikipedia.org/wiki/Osa…
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πŸ‘€︎ u/outdoorphil
πŸ“…︎ Oct 22 2018
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[#4|+4882|1955] TIL: The discoverer of a gene for Green Fluorescent Protein lost his grant, didn't get tenure, left academe and was working at a car dealership in Huntsville, Alabama, when he learned that former colleagues had won the Nobel Prize using the gene he sequenced. [/r/todayilearned] reddit.com/r/todayilearne…
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πŸ‘€︎ u/FrontpageWatch
πŸ“…︎ Nov 14 2014
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This is Aequorea victoria, a bioluminescent jellyfish. They’re incredibly important to science as they make a fluorescent green protein that lets biologists study gene expression
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πŸ‘€︎ u/the_karma_llama
πŸ“…︎ Sep 05 2019
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Bacteria expressing Green Fluorescent Protein (GFP), viewed under blue light
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πŸ‘€︎ u/bluesberryblue
πŸ“…︎ Aug 12 2019
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TIL guy who discovered Green fluorescent protein (GFP), for which three other scientists received a Nobel prize in 2008, works as a driver earning $10/hour capecodonline.com/apps/pb…
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πŸ‘€︎ u/oshen
πŸ“…︎ Jun 05 2012
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Swell Sharks: scientists have discovered the shark (Cephaloscyllium ventriosum) actually glows bright green thanks to fluorescent proteins inside its skin which are activated by blue light – the wavelength of visible light that is least absorbed as it travels through water. v.redd.it/ur2yj1cqiyp21
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πŸ‘€︎ u/hercs247
πŸ“…︎ Apr 03 2019
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Transgenic cat is glowing. Caused by GFP - green fluorescent protein
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πŸ‘€︎ u/glomeratus
πŸ“…︎ Jun 03 2017
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0.5l of bacteria pellets. The one on the left contained a GFP (Green Fluorescent Protein)-tagged protein, which gave it this nice colour.
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πŸ‘€︎ u/Arriachi
πŸ“…︎ Feb 06 2019
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Genetic scientists will often include a gene that codes for a green fluorescent protein in combination with a desired gene. How does the glow tell scientists that the desired gene was inserted correctly?
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πŸ‘€︎ u/MYC0B0T
πŸ“…︎ Apr 20 2016
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The Crystal jelly (Aequorea victoria) is a jellyfish that is found off the west coast of North America. The species is best known as the source of two proteins involved in bioluminescence; aequorin and green fluorescent protein which lead to their discoverers winning the Nobel Prize in Chemistry.
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πŸ‘€︎ u/FillsYourNiche
πŸ“…︎ Apr 11 2017
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Green Fluorescent Protein/Fluorescent Microscopy

Hi! I am working on a technique overview research paper regarding GFP/Fluorescent Microscopy. I have pulled some articles from PubMed that have me headed in the right direction. The paper is pretty straightforward; history, usage, future potential, etc. If anyone knows of anything new/unusual that would be of great help. Additionally, please let me know if you are familiar with any work that has been discredited. Thanks in advance!

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πŸ‘€︎ u/fischervol
πŸ“…︎ Apr 09 2017
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[ASAP] Mechanism of Color and Photoacidity Tuning for the Protonated Green Fluorescent Protein Chromophore

Journal of the American Chemical SocietyDOI: 10.1021/jacs.0c02796

https://ift.tt/2MIcISG

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πŸ‘€︎ u/TomisMeMyselfandI
πŸ“…︎ Jun 09 2020
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[ASAP] Room-Temperature Dual Fluorescence of a Locked Green Fluorescent Protein Chromophore Analogue

Journal of the American Chemical SocietyDOI: 10.1021/jacs.9b05096

https://ift.tt/39pBnG5

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πŸ‘€︎ u/TomisMeMyselfandI
πŸ“…︎ Dec 31 2019
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