Hello fellow lurkers
I am pretty sure you all have read about the 3 alpha-hydroxysteroid reductase theory by a fellow follower.
I am sure a lot of you think that it's something legit and has the potential to end all our problems.
I am of the belief that if we somehow work together as a community and look into this we really might have something worthwhile here.
Anyone know if the suppression of 5 alpha reductase during Accutane treatment is permanent after treatment ends?
Journal of the American Chemical SocietyDOI: 10.1021/jacs.1c01359
Fabian Hecker, JoAnne Stubbe, and Marina Bennati
https://ift.tt/3tr9yGb
Hello World,
Can anyone provide me with some information about possible interactions (or recommendatios in general) of mRNA vaccine and 5-alpha reductase inhibitors (e.g. finasteride)? I'm trying to find something about this topic for the last three days, but can't find anything.
I'm asking this because I was scheduled to get Moderna vaccine on 5th of May. When I told the physician that I take finasteride (1 mg/day for 7 years now) she told me that I cannot get the jab. The reason that she gave was that she checked on the Internet (sic!) That finasteride is a steroid and these drugs tend to supposedly weaken the production of antibodies. If I want the vaccine I should stop taking the drug for at least 15 days before the first dose.
I'm not sure if I'm right but is it possible that she didn't understand what finasteride accutaly is and what purpose it serves/ what is its mode of action? I know steroids for their antiinflammatory action but I not sure if 5-alpha reductase inhibitors work the same way?
Any thoughts on that? Stay safe.
Journal of the American Chemical SocietyDOI: 10.1021/jacs.1c02152
Clorice R. Reinhardt, Elvira R. Sayfutyarova, Jiayun Zhong, and Sharon Hammes-Schiffer
https://ift.tt/32liHom
Robust [NiFe] hydrogenase 1 (Hyd1) from Escherichia coli is shown to have non‐native, H2‐dependent activity for FMN and FAD reduction. It is a promising recycling system for FMNH2 or FADH2 supply to flavoenzymes for chemical synthesis when coupled with an Old Yellow Enzyme ene‐reductase.
Abstract
A new activity for the [NiFe] uptake hydrogenase 1 of Escherichia coli (Hyd1) is presented. Direct reduction of biological flavin cofactors FMN and FAD is achieved using H2 as a simple, completely atom‐economical reductant. The robust nature of Hyd1 is exploited for flavin reduction across a broad range of temperatures (25–70 °C) and extended reaction times. The utility of this system as a simple, easy to implement FMNH2 or FADH2 regenerating system is then demonstrated by supplying reduced flavin to Old Yellow Enzyme “ene‐reductases” to support asymmetric alkene reductions with up to 100 % conversion. Hyd1 turnover frequencies up to 20.4 min−1 and total turnover numbers up to 20 200 were recorded during flavin recycling.
https://ift.tt/38KXibI
I would like to preface that I am not posting this in search of medical advice. However, I am looking for more information in order to make a decision that COULD have impacts on my health.
Questions:
-
In what way do 5α-reductase inhibitors affect the brain?
-
I am aware of potential sex-related side effects of 5α-reductase inhibitors, but would they be exacerbated by a history of TBIs/concussions?
-
If saw palmetto and finasteride are both 5α-reductase inhibitors, do they necessarily have the same mechanisms of action - conversely if you take a similar dosage of the two and don't experience any noticeable side effects, is unlikely that you will experience side effects with the other?
-
Would people with a history of TBIs/concussions be necessarily more at risk of developing things like brain fog, depression, dementia, etc. from the use of 5α-reductase inhibitors? How and why?
I apologize for the lengthy post. My questions are primarily related to the brain, but if this is the wrong subreddit for this, please let me know. Thank you in advance for your help - answering as many of these questions as you can would be appreciated!
Background information about me: I am a 20-year-old male university student who has noticed a receding hairline over the past two years. My father and maternal uncle and grandfather are all bald, and my father also started balding in his late teens. My receding hairline is the only thing that I see when I look in the mirror or when I am on Facetime or Zoom calls, which is why I recently reached out to a doctor about Finasteride (I was prescribed to take 1/4 Proscar tablets (1.25mg Finasteride) daily, but have not yet picked up my prescription/started to take the pills.
I have had two minor concussions (no loss of consciousness, although the second one took months to recover from, likely due to stress and poor management of the concussion in the early days) at the ages of 14 and 17. I have been taking saw palmetto orally for the past ~3.5 years on the recommendation of my mother without understanding the mechanism behind it (as I was just a teenager, taking my mother's word - I only found out what a 5α-reductase inhibitor was in the past week). Within those 3.5 years, there was a 3-month period this year where I stopped taking it (simply because it ran out) and no significant changes were noticed. I have had depressive periods in my life in my teen years (although I seemed to have depressive tendencies bef
... keep reading on reddit ➡Based on New theory on this subreddit
The E. coli ribonucleotide reductase (RNR), a paradigm for class Ia enzymes including human RNR, catalyzes the biosynthesis of DNA building blocks and requires a di‐iron tyrosyl radical (Y122•) cofactor for activity. The knowledge on in vitro Y122• structure and its radical distribution within ß2 subunit has accumulated over the years; yet, little information exists on in vivo Y122•. Here, we characterize this essential radical in whole cells. Multi‐frequency EPR and electron‐nuclear double resonance (ENDOR) demonstrate that the structure and electrostatic environment of Y122• are identical under in vivo and in vitro conditions. Pulsed dipolar EPR experiments shed light on a distinct in vivo Y122• per ß2 distribution supporting the key role of Y• concentrations in regulating RNR activity. Additionally, we spectroscopically verify the generation of an unnatural amino acid radical, F3Y122•, in whole cells, providing a crucial step towards unique insights into the RNR catalysis under physiological conditions.
https://ift.tt/2Qju91e
Ive been reading things about it. I started taking finasteride one week ago. The sides Im having is a growth of my eyelashes, and wattery semen. I felt a bit more energetic and angry the first few days but it seems to go away.
Ive read a lot of stuffs online that scare me, my concern in the long term:
-Depression -Brain damages due to the lack of dht for neurosynthesis
The sexual sides effect dont bother me so much but Im afraid of brain damages.
Journal of the American Chemical SocietyDOI: 10.1021/jacs.1c01086
Anjali Patwardhan, Ritimukta Sarangi, Bojana Ginovska, Simone Raugei, and Stephen W. Ragsdale
https://ift.tt/3d19W7Y
Hey hope you all are doing fine. I've been searching up about increasing 5a-reductase activity for more conversion of Testosterone into DHT but I can only find ways to inhibit it. Would be helpful if you could share some information regarding it.
The asymmetric synthesis of N‐substituted α‐amino esters has been achieved, in high yields and excellent enantioselectivities, employing sequence diverse metagenomic imine reductases (IREDs).
Abstract
N‐Substituted α‐amino esters are widely used as chiral intermediates in a range of pharmaceuticals. Here we report the enantioselective biocatalyic synthesis of N‐substituted α‐amino esters through the direct reductive coupling of α‐ketoesters and amines employing sequence diverse metagenomic imine reductases (IREDs). Both enantiomers of N‐substituted α‐amino esters were obtained with high conversion and excellent enantioselectivity under mild reaction conditions. In addition >20 different preparative scale transformations were performed highlighting the scalability of this system.
https://ift.tt/3l0EdaD
Journal of the American Chemical SocietyDOI: 10.1021/jacs.1c01618
Braddock A. Sandoval, Phillip D. Clayman, Daniel G. Oblinsky, Seokjoon Oh, Yuji Nakano, Matthew Bird, Gregory D. Scholes, and Todd K. Hyster
https://ift.tt/2ZWYtA4
An unusual reductase (mnOpccR) in phytosterol catabolism is found to catalyze the formation of 4‐HBC through two different routes. Inactivation or overexpression of mnOpccR leads to exclusive production of 4‐androstenedione (4‐AD) and 4‐HBC from phytosterols. By a two‐step synthesis, 4‐HBC can be further efficiently converted into progesterone (PG). This work provides two green and economical methods for 4‐AD and PG manufacturing.
Abstract
4‐Androstenedione (4‐AD) and progesterone (PG) are two of the most important precursors for synthesis of steroid drugs, however their current manufacturing processes suffer from low efficiency and severe environmental issues. In this study, we decipher a dual‐role reductase (mnOpccR) in the phytosterols catabolism, which engages in two different metabolic branches to produce the key intermediate 20‐hydroxymethyl pregn‐4‐ene‐3‐one (4‐HBC) through a 4‐e reduction of 3‐oxo‐4‐pregnene‐20‐carboxyl‐CoA (3‐OPC‐CoA) and 2‐e reduction of 3‐oxo‐4‐pregnene‐20‐carboxyl aldehyde (3‐OPA), respectively. Inactivation or overexpression of mnOpccR in the Mycobacterium neoaurum can achieve exclusive production of either 4‐AD or 4‐HBC from phytosterols. By utilizing a two‐step synthesis, 4‐HBC can be efficiently converted into PG in a scalable manner (100 gram scale). This study deciphers a pivotal biosynthetic mechanism of phytosterol catabolism and provides very efficient production routes of 4‐AD and PG.
https://ift.tt/36rIWg0
https://raypeatforum.com/community/threads/glycine-strongly-upregulates-5-alpha-reductase-5-ar-activity.9361/
*I did read that it primarily upregulates 5ar1 vs 5ar2
Has anyone supplemented with Glycine, and noticed increased hair fall?
Saw this posted on Longecity.org
I'm about to take this stuff internally but I read a few people saying that it can cause "post-Finasteride syndrome" from lowered DHT like effects like depression, anxiety, impotence, erectile dysfunction, decreased libido due to the fact it's an 5α-Reductase Inhibitor.(at least what I read online it's a 5α-Reductase Inhibitor)
https://en.wikipedia.org/wiki/5%CE%B1-Reductase_inhibitor
User "Hyperflux" posted in the other GHK-CU thread that he was having these effects and they weren't going away.
How many of you have experienced such effects and did they go away over time?
Enzymatic esterification reactions under aqueous conditions are not common, as most of the well‐known enzymes catalyzing esterification require the minimization of water or activated substrates for activity. This work reports a new reaction catalyzed by carboxylic acid reductase (CAR), an enzyme known to transform a broad spectrum of carboxylic acids into aldehydes, with the use of ATP, Mg 2+ , and NADPH as co‐substrates. When NADPH was replaced by a nucleophilic alcohol, CAR from Mycobacterium marinum can catalyze esterification under aqueous conditions at room temperature. Addition of imidazole, especially at pH 10.0, significantly enhanced ester production. In comparison to other esterification enzymes such as acyltransferase and lipase, CAR gave higher esterification yields in direct esterification under aqueous conditions. The scalability of CAR catalyzed esterification was demonstrated for the synthesis of cinoxate, an active ingredient in sunscreen, with an isolated yield of 32%. The CAR esterification reported here offers a new method for green esterification under high water content conditions.
https://ift.tt/2VehH20
A new activity for the [NiFe] uptake hydrogenase 1 of Escherichia coli (Hyd1) is presented. Direct reduction of biological flavin cofactors FMN and FAD is achieved using H 2 as a simple, completely atom‐economical reductant. The robust nature of Hyd1 is exploited for flavin reduction across a broad range of temperatures (25–70 °C) and extended reaction times. The utility of this system as a simple, easy to implement FMNH 2 or FADH 2 regenerating system is then demonstrated by supplying reduced flavin to Old Yellow Enzyme ‘ene‐reductases’ to support asymmetric alkene reductions with up to 100% conversion. Hyd1 turnover frequencies up to 20.4 min ‐1 and total turnover numbers up to 20,200 were recorded during flavin recycling.
https://ift.tt/38KXibI
An unusual reductase (mnOpccR) in phytosterol catabolism is found to catalyze the formation of 4‐HBC through two different routes. Inactivation or overexpression of mnOpccR leads to exclusive production of 4‐androstenedione (4‐AD) and 4‐HBC from phytosterols. By a two‐step synthesis, 4‐HBC can be further efficiently converted into progesterone (PG). This work provides two green and economical methods for 4‐AD and PG manufacturing.
Abstract
4‐Androstenedione (4‐AD) and progesterone (PG) are two of the most important precursors for synthesis of steroid drugs, however their current manufacturing processes suffer from low efficiency and severe environmental issues. In this study, we decipher a dual‐role reductase (mnOpccR) in the phytosterols catabolism, which engages in two different metabolic branches to produce the key intermediate 20‐hydroxymethyl pregn‐4‐ene‐3‐one (4‐HBC) through a 4‐e reduction of 3‐oxo‐4‐pregnene‐20‐carboxyl‐CoA (3‐OPC‐CoA) and 2‐e reduction of 3‐oxo‐4‐pregnene‐20‐carboxyl aldehyde (3‐OPA), respectively. Inactivation or overexpression of mnOpccR in the Mycobacterium neoaurum can achieve exclusive production of either 4‐AD or 4‐HBC from phytosterols. By utilizing a two‐step synthesis, 4‐HBC can be efficiently converted into PG in a scalable manner (100 gram scale). This study deciphers a pivotal biosynthetic mechanism of phytosterol catabolism and provides very efficient production routes of 4‐AD and PG.
https://ift.tt/36rIWg0
