We compared the peripheral analgesic and CNS effect profiles of isovaline, baclofen, and GABA (a CNS-impermeant, unselective GABAB agonist). All three amino acids attenuated allodynia induced by prostaglandin E2 injection into the mouse hindpaw and tested with von Frey filaments.
Introduction ofΒ Ξ±βNβmethylated nonβproteinogenic amino acids into peptides can improve their biological activities, membrane permeability and proteolytic stability. This is commonly achieved, in nature and in the lab, by assembling preβmethylated amino acids. The more appealing route of methylating amide bonds is challenging. Biology has evolved anΒ Ξ±βNβautomethylating enzyme, OphMA, which acts on the amide bonds of peptides fused to its Cβterminus. Due to the ribosomal biosynthesis of its substrate, the activity of this enzyme towards peptides with nonβproteinogenic amino acids has not been addressed. An engineered OphMA, inteinβmediated protein ligation and solidβphase peptide synthesis have allowed us to demonstrate the methylation of amide bonds in the context of nonβnatural amides. This approach may have application in the biotechnological production of therapeutic peptides.
https://ift.tt/3tpeegz
I'm doing a paper and I need to know, roughly, how many amino acids have been discovered, including the non-proteinogenic ones. I had heard an estimate in the hundreds but I haven't been able to verify it. Any help?
I have a sleep disorder where I do not enter deep sleep properly (my sleep study showed I dipped moved from light sleep to REM with little stage 3 or 4). After years of research I discovered glycine promotes these stages of sleep that I was missing. Now I have almost completely managed to control it with high doses of glycine (15g, twice nightly, 30g total). I buy AjiPure since it's the only pharmaceutical grade glycine I can find. I assumed it was safe since glycine is commonly found in high amounts in foods. Any reason to think I might be wrong?
if anyone is just starting to study and would like to use an anki deck for amino acids, i made one that i feel is pretty comprehensive and covers most of what is asked about amino acids! i didn't really include any structures because i used the "Amino Acid Quiz" app to study those, but this deck might be a good place to start if anyone needs some guidance on what you need to know about AAs :)
k
For anyone looking for a guide. Let me know if I missed anything :)
Edit: created link + updates
This does not include glycine because itβs kinda both polar and non polar!
V- WAP-MILF
If I have trouble remembering the V I just think βvaginaβ. WAP stands for the cardi B song! If you want to include glycine you could also do:
Very Good - MILF-WAP
I used this when I took biochem and it immediately helped me looking over notes. With this down, if I come across an amino acid not in my list I know it is polar!
Good luck everyone :)
For an amino acid to be considered non-essential it needs to not be produced in 'significant' quantities within the human body. This is what keeps some essential amino acids from being considered non-essential, since some are produced in very small quantities. However, the criteria for 'significant' is unestablished. It is possible that some amino acids may be misleadingly classified as 'non-essential' because they are produced in the human body, but not in optimal quantities.
It may be beneficial to intake certain non-essential amino acids to supplement their inadequate endogenous production, but I am unable to find research on this aside from this paper (which talks more generally about mammals).
Any research/speculation anyone could offer on this topic would be much appreciated.
hey guys! I know we have to know the structure, three letter abbreviation, one letter code, and the acidity/basicity/nonpolar/polar for each but do I need to memorize the pKas for Lys, Arg, His, etc.? And also do I need to know if they're essential or not?
Whenever I take a large dose of any of the follow supplements I get this warm/hot cozy feeling inside.
Gaba, agmutine sulphate,NAC, taurine
It feels like it is coming from the inside of me though, like I have no desire to take off my jacket or regulate my external temperature. It feels really nice and relaxing and lasts for around 20 minutes. It makes my breathing kinda weird idrk how to describe it, but if it happens during a workout then I just sit down for a few minutes.
Anyone know what this is?
I had a pretty stupid debate while drunk that you could survive on just the following above. Was my drunk self correct, or would you need more to stay alive? My main concern was protein, but aside from that, it seems like one could survive on the above diet.
hello!
i have very dry, sensitive skin (eczema-prone on and around the eyelids) and i'm absolutely terrible at keeping up skincare routines (for this reason i'm contemplating buying smaller sizes and reducing waste). i'm also on a budget, and i gravitate towards greener options in terms of production and packaging
i don't have a skincare routine right now, but i decided to try the ordinary's squalene cleanser and their moisturiser. i've used their hyaluronic acid in the past, and while it was good, i'm curious to see if their plant-derived hemi squalene or their amino acids + b5 are better alternatives βΒ i've seen equal amounts of good reviews for both, so i don't really know where to go from here
does anyone have any suggestions? thank you! <3
I know that some amino acids like arginine are positively charged at physiological pH but when I'm trying to find the charge at a different pH (like 2 or 9 or something), is it still +1?
^
Just curious if anyone's given this a shot and what they thought of it. Seems to have a 10:1:6 ratio. More affordable than ocean grown dry amendment (which has a 14:1:3 ratio).
Though the song is excellent, there is an unfortunate error that I think we can update. Here are the original lyrics:
>Glycine, valine, leucine, isoleucine, alanine, they all are aliphatic, so you will not see a ring!
>
>The lone human amino acid with one is proline. From protein we are formed!
>
>With two hydroxyl groups you'll find serine and threonine. For sulfur, look to cysteine or to methionine!
>
>For bases, you have arginine, lysine, and histidine. From protein we are formed! (Now the aromatics!)
>
>Tryptophan and tyrosine and phenylalanine! Aspartic and glutamic are both acids as we've seen.
>
>For amides, see asparagine and also glutamine. From protein we are formed!
The problem:
"They all are aliphatic, so you will not see a ring" This statement is technically true; those 5 amino acids are aliphatic and do not contain any ring structures (heteroatoms such as carbons linked together in an enclosed ring). The issue is this implies that Aliphatic (non-aromatic) molecules NEVER have rings and ONLY Aromatic molecules have them. This is completely wrong, especially in light of the next line. "The lone human amino acid with one is Proline" which is wrong because Proline is NOT the only amino acid with a ring structure (All the aromatic AAs have ring structures), and it is NOT Aromatic. So if we were to update the line to be chemically accurate it could go something like:
>Glycine, valine, leucine, isoleucine, alanine, they all are aliphatic, AND you will not see a ring!
>
>The only aliphatic acid WITH a ring's proline. From protein we are formed!
That way the statement about proline is accurate and we aren't erroneously implying that aliphatics never have rings or that proline has the only ring out of all the amino acids.
Alright so I've had a lot of success treating many of my symptoms by supplementing with amino acids and anti inflammatories. Since I still have some symptoms left I was checking out a list of amino acids and their function to see if anything else would be worth trying. Honestly found a number that I thought seemed good. I've also read a lot about how amino acids can be depleted during times of stress and illness, and it can be hard to get individual amino acids back to a normal balance without direct supplementation of that amino acid.
So anyway I'm kind of formulating a new theory that atleast for my longhaul symptoms I either have lingering inflammation from covid stressing my body out causing protein/amino acid breakdown issues and imbalances or I have amino acid imbalances causing stress causing inflammation. Vicious cycle
Has anybody had any kind of amino acid levels testing done? They're definitely not routine at the doctor and kind of pricy, also not sure if they're super accurate; but I felt like if there was a glaring issue we saw in a lot of people that could shed some light on what's going on with longhaulers; maybe even break the cycle of stress and inflammation by addressing the imbalances.
Also I have no medical knowledge whatsoever this theory just came to me trying to research new things I could try to help my symptoms. Felt like somewhere I've heard ~every disease is caused by a nutritional deficiency/imbalance~ which got my gears turning.
For example we know that tryptophan competes with other large neutral amino acids for the BBB but what about other amino acids?
For example if I take a tablet of X-amino acid + 1 of Y-amino acid, will they compete or act synergistically? I'm looking for a reliable table of all amino acid interactions, if it does exist.
*meant to write, built with nucleotides.
Some amino acids can be used as a supplement, for example L-glutamine. Would it harm/change the molecule when adding it to hot beverages, like tea? Iβve seen some conflicting statements being made about it changing parts of the molecule rendering it useless. Do amino acids denature and if so, would this occur at temperatures below 100C?
