A list of puns related to "Metabolomics"
This post means I need to significantly cut back, I've been feeling weird pains from what I'm thinking is my liver or kidneys and my doc said there was stuff in the blood test she wanted to tell me and I put it off because my suicidal compulsion and debilitating PTSD were so severely in need of significant help. Combined with meditative practices EMDR and "parts" work therapy I'm in a significantly better place and may even be able to go back to work. I have nutmeg to thank for a great part of that; but it looks like I'm gonna have to find something else now. This is from a comment on another post and I felt it needed it's own post:
I've had phenomenal mental health benefits from microdosing in the 2-8g range in dose-weighted pulses, it's got a more psychedelic version of MDMA which is a lot more like mescaline. I'm looking up toxicity now; apparently there's data I've never looked up that came out in Nov 2020.
https://www.sciencedirect.com/science/article/pii/S0753332220309732 - Hepatotoxicity of nutmeg: A pilot study based on metabolomics:
"Although studies have reported that nutmeg has a certain therapeutic effect and even a hepatoprotective effect, (we need to find that data) it is undeniable that nutmeg shows toxicity, which may result from exposure to high doses and/or exposure compositions extracted with different methods [7]. It was reported that LD50 is 5.1 g/kg, (not sure if this is human or animal Edit: it's gotta be mice, that's way too high for humans.. no still a bit low, maybe, I've just recently done 60g and it wasn't my first, maybe I was in the other 50%, but I was ABSURDLY hungry so maybe I remediated somehow, but also these human animal conversions are not clear cut, and I did a crude one, I think there's more advanced versions) and signs of abnormal behavior such as hypoactivity, unstable gait, and dizziness are seen in animals given a dose of 4 g/kg or higher [25]. Numerous cases of nutmeg abuse and poisoning were reported in recent decades [8,10,[26], [27], [28]]. However, few studies reported the toxicity of nutmeg to the liver. To the best of our knowledge, this is the first metabolomics study on nutmeg hepatotoxicity."
"Control groups (C-7 and C-14): 0.2 mL normal saline was administered to each mouse daily by intragastrical gavage (feeding tube) for 7 and 14 days, respectively. Low-dose groups (L-7 and L-14): 1.0 g/kg nutmeg powder in 0.2 mL normal saline was administered to each mouse daily by intragastrical gavage for
... keep reading on reddit ➡WARNING Preprint! Not peer-reviewed!
https://www.biorxiv.org/content/10.1101/2021.12.29.474437
Background
Systemic treatments for prostate cancer (PC) have significant side effects. Thus, newer alternatives with fewer side effects are urgently needed. Animal and human studies suggest the therapeutic potential of low carbohydrate diet (LCD) for PC. To test this possibility, Carbohydrate and Prostate Study 2 (CAPS2) trial was conducted in PC patients with biochemical recurrence (BCR) after local treatment to determine the effect of a 6-month LCD intervention vs. usual care control on PC growth as measured by PSA doubling time (PSADT). We previously reported the LCD intervention led to significant weight loss, higher HDL, and lower triglycerides and HbA1c with a suggested longer PSADT. However, the metabolic basis of these effects are unknown.
Methods
To identify the potential metabolic basis of effects of LCD on PSADT, serum metabolomic analysis was performed using baseline, month 3, and month 6 banked sera to identify the metabolites significantly altered by LCD and that correlated with varying PSADT.
Results
LCD increased the serum levels of ketone bodies, glycine and hydroxyisocaproic acid. Reciprocally, LCD reduced the serum levels of alanine, cytidine, asymmetric dimethylarginine (ADMA) and 2-oxobutanoate. As high ADMA level is shown to inhibit nitric oxide (NO) signaling and contribute to various cardiovascular diseases, the ADMA repression under LCD may contribute to the LCD-associated health benefit. Regression analysis of the PSADT revealed a correlation between longer PSADT with higher level of 2-hydroxybutyric acids, ketone bodies, citrate and malate. Longer PSADT was also associated with LCD reduced nicotinamide, fructose-1, 6-biphosphate (FBP) and 2-oxobutanoate.
Conclusion
These results suggest a potential association of ketogenesis and TCA metabolites with slower PC growth and conversely glycolysis with faster PC growth. The link of high ketone bodies with longer PSADT supports future studies of ketogenic diets to slow PC growth.
Since many features of metlin are now only comercially available , I'm looking for a replacement metabolome database for untargeted LC-MS. It may also be a commercial solution, but before buying Metlin Gen2 I thought I'd ask if anyone has good recomendations.
Abstract
Our aim was to determine the effect of diet on gut microbiota, digestive function and sensations, using an integrated clinical, metagenomics and metabolomics approach. We conducted a cross-over, randomised study on the effects of a Western-type diet versus a fibre-enriched Mediterranean diet. In 20 healthy men, each diet was administered for 2 weeks preceded by a 2-week washout diet. The following outcomes were recorded: (a) number of anal gas evacuations; (b) digestive sensations; (c) volume of gas evacuated after a probe meal; (d) colonic content by magnetic resonance imaging; (e) gut microbiota taxonomy and metabolic functions by shotgun sequencing of faecal samples; (f) urinary metabolites using untargeted metabolomics. As compared to a Western diet, the Mediterranean diet was associated with (i) higher number of anal gas evacuations, (ii) sensation of flatulence and borborygmi, (iii) larger volume of gas after the meal and (iv) larger colonic content. Despite the relatively little difference in microbiota composition between both diets, microbial metabolism differed substantially, as shown by urinary metabolite profiles and the abundance of microbial metabolic pathways. The effects of the diet were less evident in individuals with robust microbiotas (higher beta-diversity). To conclude, healthy individuals tolerate dietary changes with minor microbial modifications at the composition level but with remarkable variation in microbial metabolism.
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