I attempted to make some competent BL21(DE3)pLysS cells by taking 10ul of competent stock cells and growing them O/N in 6ml LB with 1:1000 25mg/ml of chloramphenicol.
I then transferred this pre-culture to 250ml of LB and harvested 30ml of the cells at an OD of ~0.4 by cebtrifugation for 10 minutes at 3000 rpm. I resuspended the pellet in 3ml of TSS buffer, however, when I made the buffer I realised later that I grabbed manganese chloride instead of magnesium chloride. Anyway, I froze the cells and attempted two transformations later that day with an ampicillin and a kynamycin resistant vector via heat shock at 42°C for 50 seconds. I plated out the transformant on the appropriate agar, and incubated them O/N, and I have a metric bucket tonne of colonies. I'm going to do a miniprep on some and send the product for sequencing to see if the colonies do actually have the plasmid in them, but what the fuck? Without magnesium chloride the cells shouldn't be competent right? Anyone got any ideas as to what's going on?
Is it possible to make Manganese(II) sulfate tetrahydrate from pure manganese if so, how?
I'm having one more allergy test procedure on Monday but today's findings suggest that I'm allergic to cobalt(ii)chloride hexahydrate and possibly nickel. Any suggestions on how to curb my interaction with things that contain both cobalt(ii)chloride and nickel? I'm kind of at a loss on where to start.
I've been dealing with my dyshidrosis since the summer of 2017 but my flare-ups have been more consistent and fiery over the past year.
The lab sheet tells me that I can reduce a manganse compund (mno2) to Mn2+ by using SO3-2 but I don't see how. Sure, if I mix it with H2so4, it will leave a Mn2+ ion behind. But I don't see how a sulfite would do the job.
i've had dyshidrosis flare-ups for a few years now but they were never very serious or noticeable, just a few bubbles here or there on my fingers!
this past year, i've had flare-ups consistently last months at a time and i couldn't nail down any of my triggers.
rather recently, i was referred to an allergy specialist to do some patch tests! i found out i was allergic to cobalt ii chloride and had a subsequent nickel sensitivity. however, my biggest trigger ended up being LANOLIN which showed up later in my patch test.
lanolin is apparently in a SHIT TON of cosmetics and other things and i realized that it was both in the facial cleanser i'd been using for a year [st. ives avocado & honey scrub] as well as in my translucent powder [coty airspun] used to set my foundation!
my fingers are still in the process of healing from my last flare-up but no new bubbles have shown up since i cut those two products out.
ik these are specific triggers to me but if i can help someone sus out their own triggers by hearing my experience, i'll be super stoked. 🌸
ZIF‐8 membrane separation performance is modified by a facile vapor‐phase metal–organic treatment. Significant increase in selectivity for propylene/propane and hydrogen over other gases is demonstrated at the expense of reduced flux.
Abstract
Vapor‐phase treatment of ZIF‐8 membranes with manganese(II) acetylacetonate (Mn(acac)2) allows permselectivity tuning. Propylene/propane selectivity increases from 31 to 210 after the Mn(acac)2 treatment at 165 °C for 30 min, while selectivities increase from 14.6 to 242 for H2/CH4, from 2.9 to 38 for CO2/CH4, from 2.4 to 29 for CO2/N2, and from 2.9 to 7.5 for O2/N2, after Mn(acac)2 treatment at 175 °C for 30 min. Stable equimolar propylene/propane mixture selectivity of 165 at ambient temperature and 4 bar equimolar feed with a propylene flux of 8.3×10−4 mol m−2 s−1 is established. A control experiment excludes thermal treatment alone causing these changes. XPS analysis reveals the presence of Mn(acac)2 on the outer surface of the vapor‐treated ZIF‐8 membranes while no other changes are detectable by X‐ray diffraction and infrared spectroscopy.
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