Score with the Scaffold Puns

I've only recently came back to Minecraft and there is so many new things added to the game since I last played.

I don't understand scaffolds though and the point of them. Am I missing something or is there more that can be done with them?

All‐DNA energy conversion: ZnIIPPIX/G‐quadruplex aptamer/electron acceptor nanostructures provide effective scaffolds for mimicking photosynthesis.

Abstract

All‐DNA scaffolds act as templates for the organization of photosystem I model systems. A series of DNA templates composed of ZnII‐protoporphyrin IX (ZnIIPPIX)‐functionalized G‐quadruplex conjugated to the 3′‐ or 5′‐end of the tyrosinamide (TA) aptamer and ZnIIPPIX/G‐quadruplex linked to the 3′‐ and 5′‐ends of the TA aptamer through a four‐thymidine bridge. Effective photoinduced electron transfer (ET) from ZnIIPPIX/G‐quadruplex to bipyridinium‐functionalized tyrosinamide, TA‐MV2+, bound to the TA aptamer units is demonstrated. The effectiveness of the primary ET quenching of ZnIIPPIX/G‐quadruplex by TA‐MV2+ controls the efficiency of the generation of TA‐MV+.. The photosystem‐controlled formation of TA‐MV+. by the different photosystems dictates the secondary activation of the ET cascade corresponding to the ferredoxin‐NADP+ reductase (FNR)‐catalysed reduction of NADP+ to NADPH by TA‐MV+., and the sequestered alcohol dehydrogenase catalysed reduction of acetophenone to 1‐phenylethanol by NADPH.

https://ift.tt/2uR1SVf

Journal of the American Chemical SocietyDOI: 10.1021/jacs.0c01003

https://ift.tt/2uQVCgn

All‐DNA scaffolds act as templates for the organization of photosystem I (PS I) model systems. A series of DNA templates composed of Zn(II)‐protoporphyrin IX (Zn(II)PPIX)‐functionalized G‐quadruplex conjugated to the 3 ' ‐ or 5 ' ‐end of the tyrosinamide (TA) aptamer and Zn(II)PPIX/G‐quadruplex linked to the 3 ' ‐ and 5 ' ‐ends of the tyrosinamide aptamer through a four‐thymidine (4×T) bridge, structures I to IV. Effective photoinduced electron transfer (ET) from Zn(II)PPIX/G‐quadruplex to bipyridinium functionalized tyrosinamide, TA‐MV 2+ , bound to the TA aptamer units is demonstrated. The order of photosensitized ET quenching in the photosystems is III > IV > I > II and follows the binding affinities of TA‐MV 2+ to the aptamer units. The effectiveness of the primary ET quenching of Zn(II)PPIX/G‐quadruplex by TA‐MV 2+ controls the efficiency of the generation of TA‐MV +• , under steady‐state illumination, in the presence of 2‐mercaptoethanol as sacrificial electron donor. The photosystem‐controlled formation of TA‐MV +• by the different photosystems dictates the secondary activation of the ET cascade corresponding to the ferredoxin‐NADP + reductase, FNR, catalysed reduction of NADP + to NADPH by TA‐MV +• , and the sequestered alcohol dehydrogenase catalysed reduction of acetophenone to 1‐phenylethanol by NADPH. The photosensitizer‐aptamer conjugates reveal switchable “ON”/“OFF” photoactivities. Also, a photosystem consisting of Zn(II)PPIX/G‐quadruplex linked to the 3 ' ‐ and 5 ' ‐ends of two TA aptamer units, structure V, revealing the improved photoinduced ET quenching and subsequent biocatalytic cascades, is presented. Scaling of the all‐DNA photosensitizer‐aptamer/electron acceptor is achieved by the rolling‐circle‐amplified (RCA) synthesis of micrometre‐long chains consisting of Zn(II)PPIX/G‐quadruplex linked to two TA aptamer repeat units.

https://ift.tt/2uR1SVf

Recently, I've been building a lot of smaller apps in Ruby, and as a result, I've been wasting too much time setting up the same project structure for each of them, so I decided to write a tool for automating this process.

Since it generates only the essentials needed to start working, I thought it might be useful to others on this subreddit. You can install it by running gem install create-ruby-app, and the source code is available at https://github.com/majjoha/create-ruby-app.

A few examples:

$ create-ruby-app new my_app

$ create-ruby-app new web_app -g sinatra,sequel -r ruby-2.6.0

$ create-ruby-app new --ruby jruby-2.9.6.0 my_app

My problem is that, even as an ARc subclass, in the 1300 today in Perdition, there's too many arc shields. I don't own an Arc Bow, so that sucks. I make do regardless. Then I get to the Overload Minotaur, and there's nothing I can do. I had to run an Arc SMG for the shields, so I don't have Le Monarque to use, which makes Overloads TRIVIAL. I'm running Dead Man's Tale in the primary slot so I can deal with Shields.

I have no damage to kill the Overload before the stun wears off, and trying to stun it with an SMG wile it's blinking, and I die in two hits, is incredibly hard. Obviously I'm a bit under light, I'm about 1275, but maybe there's a secret loadout for Arc titan I'm not thinking of here? I'm fine with taking my time, but I've heard that score affects the exotic drop rate. I don't want to spend forty minutes doing this grueling dungeon with one life at the end just to get nothing from it.

In .net core 3.1 MVC project, i want to scaffold user registration (sign-up, login etc) but without that garbage pagelmodel and razor pages. How do i do it ?