A list of puns related to "Nucleic acid quantitation"
I can recogonize them if shown them, but I have been really struggling to draw them out precisely. Like I know I can draw out the rough features and I know how to differentiate between them.
Important updates to this post including that a clinical trial was based on "misreported data" and the fact #Exicure CEO David Giljohann, one of the developers of the #SphericalNucleicAcids in Chad Mirkin's group is "separating" from the company.
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Nucleic acids are chemically modified to fine-tune their properties for biological function. Chemical tools for selective tagging of base modifications enables new approaches; the photosensitizers riboflavin and anthraquinone were previously proposed to oxidize N6-methyladenine (m6A) or 5-methylcytosine (5mdC) selectively. Herein, riboflavin, anthraquinone, or Rose Bengal were allowed to react with the canonical nucleosides dA, dC, dG, and dT, and the modified bases 5mdC, m6A, 8-oxoguanine (dOG), and 8-oxoadenine (dOA) to rank their reactivities. The nucleoside studies reveal that dOG is the most reactive and that the native nucleoside dG is higher or similar in reactivity to 5mdC or m6A; competition in both single- and double-stranded DNA of dG vs. 5mdC or 6mdA for oxidant confirmed that dG is favorably oxidized. Thus, photosensitizers are promiscuous nucleic acid oxidants with poor chemoselectivity that will negatively impact attempts at targeted oxidation of modified nucleotides in cells.
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