Does it require an endonuclease or exonuclease? Confused because one of the Bouras anki cards said it uses an exonuclease, but UEarth says it uses an endonuclease
In prokaryotes, MutH creates a nick in a newly - synthetized strand containing a mismatched nucleotide, thus allowing the exonuclease to degrade the portion of this strand containing the error.
But what about eukaryotes? How is this particular nick in the daughter strand formed?
Thanks in advance!
From what I understood from google, mismatch repair removes and corrects only one base at a time, while in nucleotide excision repair a whole segment of DNA is also excised, not just the problematic base. Is this right? Also are there any other differences between the two?
(Context: Was reading Robbins neoplasms and it mentioned that HNPCC had defects in DNA mismatch repair, while Xeroderma Pigmentosum has defects in nucleotide excision repair. Then I realised I don't remember the difference btw the two repair mechs >_<)
My mother has a mutated mismatch DNA repair gene.
While searching for information regarding this and its links to cancer, there doesn't seem to be a lot of recent interest on the topic (most stuff is from the 1990s and the most recent study I'm aware of is something from 2012), or maybe researchers simply don't have enough patients with those specific mutations to research. Is further research on this topic beneficial to the scientific community or is it only useful for the particular person with the mutation? Please explain. Thank you so much!
I know that mismatch repair proteins (e.g. MutS homolog 2) recognize changes in topology due to DNA mismatches and correct them within the mutS complex, but how does it know which is the correct base and which the wrong one so as to avoid a single base-pair mutations?
Can anyone help clarify to me the differences between these DNA mechanisms? Any help would be greatly appreciated :)
