Terahertz Time Domain Spectroscopy Puns

Characterization of Porous Silicon Using Terahertz Differential Time-Domain Spectroscopy by Suchitra Ramani, Alan Cheville, J. Escorcia Garcia, and Vivechana Agarwal

http://dx.doi.org/10.1364/OTST.2007.TuA5

Thanks in advance!

Journal of the American Chemical SocietyDOI: 10.1021/jacs.1c02545

Hikaru Kuramochi and Tahei Tahara

https://ift.tt/3gpnM5Y

Journal of the American Chemical SocietyDOI: 10.1021/jacs.1c03314

Aurelio J. Dregni, Harrison K. Wang, Haifan Wu, Pu Duan, Jia Jin, William F. DeGrado, and Mei Hong

https://ift.tt/3boYT8F

Journal of the American Chemical SocietyDOI: 10.1021/jacs.1c02149

Brandon H. Bowser, Shu Wang, Tatiana B. Kouznetsova, Haley K. Beech, Bradley D. Olsen, Michael Rubinstein, and Stephen L. Craig

https://ift.tt/3sDvNZB

Journal of the American Chemical SocietyDOI: 10.1021/jacs.1c08103

Gary K. Chow, Archana G. Chavan, Joel Heisler, Yong-Gang Chang, Ning Zhang, Andy LiWang, and R. David Britt

https://ift.tt/3mSNunm

A cryo‐EM study to investigate the cooperativity of N‐terminal domain (NTD) up/down conformational transitions in an R95G homo‐hexameric disease mutant of p97 is presented. The 14 unique conformational states of the complex were successfully classified and their populations fit to a model to address the cooperativity of the NTD transitions.

Abstract

The hexameric p97 enzyme plays an integral role in cellular homeostasis. Large changes to the orientation of its N‐terminal domains (NTDs), corresponding to NTD‐down (p97‐ADP) or NTD‐up (p97‐ATP), accompany ATP hydrolysis. The NTDs in a series of p97 disease mutants interconvert rapidly between up and down conformations when p97 is in the ADP‐bound state. While the populations of up and down NTDs can be determined from bulk measurements, information about the cooperativity of the transition between conformations is lacking. Here we use cryo‐EM to determine populations of the 14 unique up/down NTD states of the homo‐hexameric R95G disease‐causing p97 ring, showing that NTD orientations do not depend on those of neighboring subunits. In contrast, NMR studies establish that inter‐protomer cooperativity is important for regulating the orientation of NTDs in p97 particles comprising mixtures of different subunits, such as wild‐type and R95G, emphasizing the synergy between cryo‐EM and NMR in establishing how the components of p97 function.

https://ift.tt/34MkbKO

The glycan structures of the receptor binding domain of the SARS‐CoV2 spike glycoprotein expressed in human HEK293F cells have been studied by using NMR. The different possible interacting epitopes have been deeply analysed and characterized, providing evidence of the presence of glycan structures not found in previous MS‐based analyses. The interaction of the RBD 13C‐labelled glycans with different human lectins, which are expressed in different organs and tissues that may be affected during the infection process, has also been evaluated by NMR. In particular, 15N‐labelled galectins (galectins‐3, ‐7 and ‐8 N‐terminal), Siglecs (siglec‐8, siglec‐10), and C‐type lectins (DC‐SIGN, MGL) have been employed. Complementary experiments from the glycoprotein perspective or from the lectin’s point of view have permitted to disentangle the specific interacting epitopes in each case. Based on these findings, 3D models of the interacting complexes have been proposed.

https://ift.tt/3inKWcF

I read on Wikipedia and some other sources that: "NIR laser sources provide an amplitude modulated sinusoid at frequencies near one hundred megahertz (100 MHz)".

I also read that NIRS light is typically in the range of 700 - 2500 nm, which corresponds to a much, much higher frequency than the previously mentioned 100 MHz. This doesn't make much sense to me. Am I not understanding the initial statement, or is my conversion wrong? Maybe it would help if I knew what exactly is meant by "amplitude modulated sinusoid".

Thank you.