Hydrophobic Interaction Chromatography

came across a question regarding this on altius. i haven't heard of this technique before but can someone explain it and what would elute first? They said that if you decrease the salt concentration, it would elute first but i was thinking that if you add salt, that it would elute faste...

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πŸ‘€︎ u/yungsopha
πŸ“…︎ Aug 02 2021
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[ASAP] Hydrophobic Interactions between DNA Duplexes and Synthetic and Biological Membranes

Journal of the American Chemical SocietyDOI: 10.1021/jacs.0c13235

Sioned F. Jones, Himanshu Joshi, Stephen J. Terry, Jonathan R. Burns, Aleksei Aksimentiev, Ulrike S. Eggert, and Stefan Howorka

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πŸ‘€︎ u/TomisMeMyselfandI
πŸ“…︎ May 21 2021
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Question regarding hydrophobic interaction column

Which change in a solution composition would cause a protein to elite from hydrophobic interaction column? Decrease pH, increase pH, decrease salt concentration or increase salt concentration I put increase salt concentration because well salt is NaOH and if you increase that, a protein will interact with that instead of the hydrophobic interactions on the column. But answer is decrease salt concentration. I don’t get it. Please help.

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πŸ‘€︎ u/CandidSecond
πŸ“…︎ Dec 02 2020
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A Hydrophobic‐Interaction‐Based Mechanism Triggers Docking between the SARS‐CoV‐2 Spike and Angiotensin‐Converting Enzyme 2 onlinelibrary.wiley.com/d…
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πŸ‘€︎ u/dodgyb
πŸ“…︎ Nov 13 2020
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hydrophobic interactions and protein folding

Just wanted to make sure i got this correct. When hydrophobic regions are in an unfolded state the entropy tends to be low because of the cage like structure that water tends to form around these hydrophobic residues, therefore giving the protein a high delta G. But when these hydrophobic regions get buried into the core of the protein, entropy then goes up because of waters randomness around the protein. Giving us a more favourable delta G?

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πŸ‘€︎ u/whyuhavetobemad
πŸ“…︎ Sep 10 2019
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[ASAP] Genetic Encoding of -Pentafluorosulfanyl Phenylalanine: A Highly Hydrophobic and Strongly Electronegative Group for Stable Protein Interactions

Journal of the American Chemical SocietyDOI: 10.1021/jacs.0c07976

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πŸ‘€︎ u/TomisMeMyselfandI
πŸ“…︎ Oct 01 2020
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[ASAP] Ions Tune Interfacial Water Structure and Modulate Hydrophobic Interactions at Silica Surfaces

Journal of the American Chemical SocietyDOI: 10.1021/jacs.9b13273

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πŸ‘€︎ u/TomisMeMyselfandI
πŸ“…︎ Apr 01 2020
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ELI5: The relation between Van Der Waals forces and hydrophobic and hydrophilic interactions

I've heard that these are actually the same, but I don't really get it. There's also something with water being pushed out of binding pockets increasing entropy so a ligand can bind. This is a bit specific but apparently the same happens when fats get together and push out the water in between them.

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πŸ‘€︎ u/Magisidae
πŸ“…︎ Mar 05 2018
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What is the difference between hydrophobic and van der Waals interaction?

Undergraduate in biochemistry here. In one of my classes I recently completed an assignment where I had to make a bunch of figures in PyMOL highlighting various specific interactions within a particular protein. One of my figures shows interaction between a phenylalanine residue and a leucine residue. I captioned the figure "Hydrophobic interaction occurring between residues blah blah blah blah." We got our assignment back today and although I got full credit on the assignment, my professor left a comment on that figure that said "I assume you meant van der Walls interaction." Can somebody explain the difference between van der Waals and hydrophobic interaction so that I don't continue to make this mistake on future assignments?

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πŸ‘€︎ u/SatanasLucifer
πŸ“…︎ Sep 22 2015
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Hydrophobic Interactions

Could someone please explain to me why hydrophobic interactions take place in terms of entropy/enthalpy etc? Cheers in advance.

3rd year MChem student studying protein thermodynamics.

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πŸ‘€︎ u/Large_Pimpin
πŸ“…︎ Apr 17 2011
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The future of materials' interaction with water: super hydrophobic and super hydrophilous materials youtube.com/watch?v=FLegm…
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πŸ‘€︎ u/Saponetta
πŸ“…︎ Jan 21 2015
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Why are silicones and fluorocarbons hydrophobic but lipophilic? What chemical interactions are involved?

I am trying to distinguish between lipophobic and hydrophilic and I saw that the wiki article says that fluorocarbons and silicones are unique in that they are hydrophilic yet lipophobic. Can anyone clarify why this is?

Edit: my title is wrong. Can a mod edit it for me? It should rea. Hydrophilic and lipophobic. Thanks

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πŸ‘€︎ u/MysteryMeat21
πŸ“…︎ Aug 16 2013
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If a protein whose tertiary structure contains mostly hydrophobic and hydrophilic interactions is placed/comes in contact with a non-polar solution will it invert itself?
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πŸ‘€︎ u/blongey
πŸ“…︎ Apr 08 2014
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Interaction Of Hydrophobic Molecules With Water – New Insights sciencedebate.com/science…
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πŸ‘€︎ u/MySky
πŸ“…︎ Oct 04 2011
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New equation predicts molecular forces in hydrophobic interactions physorg.com/news/2011-10-…
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πŸ‘€︎ u/davidreiss666
πŸ“…︎ Oct 11 2011
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This is a 15 mm Vantablack coated ball being dipped into a bowl of water. The surface is now super-hydrophobic so water refuses to interact with it in any way v.redd.it/lbk3nylyoty21
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πŸ‘€︎ u/Mass1m01973
πŸ“…︎ May 17 2019
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This is a 15 mm Vantablack coated ball being dipped into a bowl of water. The surface is now super-hydrophobic so water refuses to interact with it in any way v.redd.it/d6l5g2abpmh11
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πŸ‘€︎ u/Mass1m01973
πŸ“…︎ Aug 22 2018
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What kind of bonds do virus protein spikes form to bond to a cell receptor?

Covalent? Polar covalent? Ionic?

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πŸ‘€︎ u/shawnwingsit
πŸ“…︎ Jan 10 2022
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This is a 15 mm Vantablack coated ball being dipped into a bowl of water. The surface is now super-hydrophobic so water refuses to interact with it in any way v.redd.it/d6l5g2abpmh11
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πŸ‘€︎ u/Mass1m01973
πŸ“…︎ Aug 22 2018
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Water interacting with super hydrophobic surface imgur.com/QU9QkY0
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πŸ‘€︎ u/shiruken
πŸ“…︎ Sep 13 2015
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This is what water interacting with super hydrophobic surface looks like v.redd.it/bmw9iosu9el11
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πŸ‘€︎ u/Mass1m01973
πŸ“…︎ Sep 10 2018
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How do hydrophobic surfaces interact with non-water liquids?

Would a hydrophobic surface or coating repel something like melted wax? Liquid nitrogen (assuming the surface was cold enough not to produce the leidenfrost effect)? Are there some types of liquid that would react differently than other types, or is there something specifically about water and water-based liquids that creates the repellant effect?

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πŸ‘€︎ u/grambino
πŸ“…︎ Oct 07 2015
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Can someone please clarify the Hydrophobic effect in the protein folding?

I understand that when protein is in primary/secondary structure there is more organization of water molecules, hence it has low entropy and when you fold it, the entropy of protein increases but the entropy of water (which becomes more positive) overcomes the entropy of folded protein. I know that proteins like hydrophobic parts inside and hydrophilic outside and that's what's confusing me. If hydrophilic residues are outside, wouldn't that interact with water and make water more organized?

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πŸ‘€︎ u/travelingcarpet
πŸ“…︎ Dec 13 2021
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This Sponge Fossil May Be the Earliest Record of Animal Life. The 890-million-year-old relic predates periods of extreme cold and the planet’s second oxygenation spike. smithsonianmag.com/scienc…
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πŸ‘€︎ u/Sariel007
πŸ“…︎ Jul 30 2021
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SAFEMOON V2 RECAP #3 πŸš€πŸŒ

Happy New year fellow safemooners!! Here it goes the first solar year of safemoon existence. It was a turbulent span of time of our lives and I hope 2022 may bring it to fruition.

A lot of things happened in the span of a few days, some of which difficult to summarize, but I will try my best. Let's begin simple and end with the difficult part.

  1. Safemoon V1 is by any means inoperable. Any action on the smart contract (buy, sell, transfer) is now taxed 100% where 1% is reflected and 99% goes to LP. Your safemoon V1 are safe and you can migrate to V2 when you want using the safemoon wallet app or the safeswap on the website. It is advisable to use the desktop version to do this as mobile is buggy. Pancakeswap did not halt transactions on V1 so don't modify the slippage because you will lose money.

  2. Two exchanges support V2 at the time of writing: KickEX and LBank. The others still have to migrate while Bitmart announced it will buy the new stack of coins, we don't know more details, negotiations are in progress. PancakeSwap is not supported by safemoon but I think it may still be used to buy V2 (need confirmation).

  3. WE HAVE A BILLBOARD UP IN TIMES SQUARE FOR 365 DAYS, on which safemoon ads will be played 3 times an hour every hour. The ads can be changed at will so this will be used for advertisement of all new products.

  4. We received a reveal video of Operation Pheonix!!! On this sub we theorized a lot about it and we were correct under a lot of aspects. I will not discuss my idea on the video here, only it was delivered on time and it was made in a few days with holidays in between. I will try to summarize the contents:

A) Operation Pheonix is sum of technological projects, of which some are discussed in the video, some not.

B) Wind turbines: the main eolic systems, featuring big turbines are CENTRALIZED meaning that one society builds them, manages them and gets the revenue. Big eolic fields are harming for the environment, as they take up space, modify the ecosystem and exterminate birds. Safemoon is working to DECENTRALIZE eolic energy production. This may be done by connecting in a single shared system (a blockchain, not mentioned in the video) thousands of small turbines that may be constructed and operated by a single man and can be installed at home. But small turbines have problems --> C

C) At safemoon are trying to make the small turbines produce more energy by increasing their efficiency. How? With hydrophobic-hydrophyllic nanoma

... keep reading on reddit ➑

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πŸ‘€︎ u/EveningCandle1025
πŸ“…︎ Jan 01 2022
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Slovenian applying ED to the US [ACCEPTED CORNELL ENGINEERING!]

Demographics

  • Gender: Male
  • Race/Ethnicity: White
  • Residence: Slovenia
  • Income Bracket: ~70k Dollars
  • Type of School: Public (IB program). Graduating class size: 32
  • Hooks (Recruited Athlete, URM, First-Gen, Geographic, Legacy, etc.): maybe, just maybe Underrepresented country

Intended Major(s): Chem Eng --> CS

Academics

  • GPA (UW/W): N/A
  • Rank (or percentile): N/A
  • IB Diploma Program:
    • PREDICTED GRADES: 44/45
      • MATH AA HL 7
      • PHYSICS HL 7
      • CHEMISTRY HL 7
      • SLOVENE HL 6
      • ENGLISH B SL 7
      • ECONOMICS SL 7

Standardized Testing

List the highest scores earned and all scores that were reported.

  • SAT I: 1450 (680RW, 770M)
    • NOTE: took only once.
  • Other (ex. IELTS, TOEFL, etc.):
    • IELTS: 8

Extracurriculars/Activities

List all extracurricular involvements, including leadership roles, time commitments, major achievements, etc.

  1. Research: Student researcher; single author, Independent chemistry research; NAME High school [10-11th grade]
    1. Gathered&read literature; investigated optimal ratio of precursors; constructed a method for synthesizing flex. aerogels; published + (see add. info)
  2. Competitor (9-12th grade), Preparations leader (11-12), School Chemistry club; Faculty of Chemistry and Chemical Technology, University of Ljubljana
    1. Attended (IChO) competition preparations; additional lab-work; learning college level chemistry; leading 11th-grade comp. preparations (see add. info)
  3. Community service: Founder & Leader of the project; chemistry tutor, NAME OF THE PROJECT - 1st high school student lead tutoring organization in Slovenia [11th-12th]
    1. Gathered tutors; build a website; contacted&offered help to 10+ schools&orphanages; taught chemistry & clarified concepts; helped over 150+ students
  4. Internship: Student researcher and software engineer - AI lab, Jozef Stefan Institute (IJS) - leading Slovenian scientific research institute [12th]
    1. Learned IA & ML fundamentals from online college courses. Learning ML model training. Collaborating in smart assistant development for oil refineries.
  5. Research: Student researcher; single author, Independent Phys-AstroEng research;NAME High school; IJS [11-12th]
    1. Studying optimal geometry of off-planet drone propellers; ran initial experiments; analyzing gathered data with software; collaborating with IJS.
  6. Internship: Student resear
... keep reading on reddit ➑

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πŸ‘€︎ u/HappyLoquat666
πŸ“…︎ Dec 14 2021
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I wonder why they deleted the r/antiwork sub

I guess the concept didn't work

πŸ‘︎ 10k
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πŸ“…︎ Jan 27 2022
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SERIOUS: This subreddit needs to understand what a "dad joke" really means.

I don't want to step on anybody's toes here, but the amount of non-dad jokes here in this subreddit really annoys me. First of all, dad jokes CAN be NSFW, it clearly says so in the sub rules. Secondly, it doesn't automatically make it a dad joke if it's from a conversation between you and your child. Most importantly, the jokes that your CHILDREN tell YOU are not dad jokes. The point of a dad joke is that it's so cheesy only a dad who's trying to be funny would make such a joke. That's it. They are stupid plays on words, lame puns and so on. There has to be a clever pun or wordplay for it to be considered a dad joke.

Again, to all the fellow dads, I apologise if I'm sounding too harsh. But I just needed to get it off my chest.

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πŸ‘€︎ u/anywhereiroa
πŸ“…︎ Jan 15 2022
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