Glycosaminoglycan Puns

I recently got curious as to why do some guys I know don't do jacksht to their skin and have clear and wrinkle-free skin?

I looked up some stuff and saw an article that said moisturizing reduces ur body's GAG(a compound that ur body naturally produces to store water or something like that" and NMFs(natural moisturizing factors) and can also cause accumulation of dead skin cells aka. slowing down skin renewal process. So they said that exfoliating is what actually helps ur skin become less dry without adding in moisturizer.

Some counter claims were that as we age our body needs help, and all the cleansing and pollutants from outside also contribute to us needing to moisturize, so that could be the reason why we need to moisturize but then would over moisturizing harm the skin if the initial claim that moisturizing can reduce ur body's GAG and NMFs is actually true or is that just bogus. Thoughts?

This is an paper that explains NMFs and GAGs but with respect to niacinamide

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2921764/

My dog just suddenly died at the vet.

I brought her in because she was limping after another dog ran into her. She's a 13yr old mini australian shepherd 45lbs and she has arthritis but other than that she is an extremely healthy dog that can keep up with even puppies at the dog park. She was on Rovera for her arthritis.

The vet gave her a shot of Polysulfated Glycosaminoglycan. A few minutes after she started vomiting so the vet gave her some sort of anti-nausea shot. She then peed/pooped herself. The vet brought her to the back and she died shortly after. He thinks it might have been a stroke but I wasn't able to get a clear answer from him.

She has never had any seizures or strokes before and was in very good health.

I just want to know if this shot had anything to do with her death. It really came out of nowhere.

I am working with some glycosaminoglycan (GAG) purification (specifically chondroitin sulfate and enzyme digest derivatives), and in order to use the enzymes I needed to use sodium phosphate as a buffer (enzymes precipitate in all other buffers i've tried). The problem is sodium phosphate is not compatible with the mass spectrometer we plan on using, so I needed to buffer swap it into ammonium acetate. This is where I am a little confused.

I figured that chondroitin sulfate can behave as an anion and I can use typical low pressure strong anion exchange (General Electric Sytiva Q FF 5ml Column). Beyond the sulfate group, there is also the hydroxyl groups on the carbohydrate itself. Now typically I would use a high concentration of sodium chloride to elute something like protein, however in this case I cannot use sodium chloride which would also be incompatible with ESI mass spec. Can I just use a high concentration of ammonium acetate to elute? I would think that it can behave as a salt, so can I use a running buffer of say 50 mM and do a linear gradient up to 400 mM? I am also using ammonium acetate pH 7, however based on some reading the glycosaminoglyans dont stick to the anion column very well, and people have reported using high pH anion exchange. However the problem is I dont want to destroy the structure of my product because I intend to elucidate the structure using mass spec.

Does anyone have any experience with GAG chemistry? Apparently this is supposed to be very routine work, however I cannot find any protocols.

A novel approach for the design of platinum complexes with intrinsic anti‐metastatic potential is presented. Metalloshielding of a model heparan sulfate (HS) pentasaccharide by the highly cationic TriPlatinNC modifies the conformational preference of the critical iduronic acid residue. Structural modulation of HS can result in inhibition of cellular cleavage by hepara(i)nase with a consequence in tumour cells being prevention of metastases.

Abstract

1H NMR spectroscopic studies on the 1:1 adduct of the pentasaccharide Fondaparinux (FPX) and the substitution‐inert polynuclear platinum complex TriplatinNC show significant modulation of geometry around the glycosidic linkages of the FPX constituent monosaccharides. FPX is a valid model for the highly sulfated cell signalling molecule heparan sulfate (HS). The conformational ratio of the 1C4:2S0 forms of the FPX residue IdoA(2S) is altered from ca. 35:65 (free FPX) to ca. 75:25 in the adduct; the first demonstration of a small molecule affecting conformational changes on a HS oligosaccharide. Functional consequences of such binding are suggested to be inhibition of HS cleavage in MDA‐MB‐231 triple‐negative breast cancer (TNBC) cells. We further describe inhibition of metastasis by TriplatinNC in the TNBC 4T1 syngeneic tumour model. Our work provides insight into a novel approach for design of platinum drugs (and coordination compounds in general) with intrinsic anti‐metastatic potential.

https://ift.tt/3klCCdp

in my biochem book it mentions that “chondroitin sulfate can form proteoglycan aggregates through NON-COVALENT ASSOCIATION with hyaluronic acid”. i was wondering what type of bonds are the “non-covalent associations” that form between HA and CS ?

Journal of the American Chemical SocietyDOI: 10.1021/jacs.0c06046

https://ift.tt/33mtxMM

PMID: 2818645

http://www.sciencedirect.com/science/article/pii/000629528990124X?via%3Dihub

My professor said that glycosaminoglycans are synthesized in the cell on proteins, and then exocytosed, but failed to elaborate on the process. Just a simple explanation would help a lot with understanding the topic, thanks!

Authors: Chandreskaan, E. V. and BeMiller, J. N.

I don't have high hopes for this one. My university's library has volumes 1 - 7, but not 8 or 9. Any help would be appreciated.

Do your worst!

My dog just suddenly died at the vet.

I brought her in because she was limping after another dog ran into her. She's a 13yr old mini australian shepherd 45lbs and she has arthritis but other than that she is an extremely healthy dog that can keep up with even puppies at the dog park. She was on Rovera for her arthritis.

The vet gave her a shot of Polysulfated Glycosaminoglycan. A few minutes after she started vomiting so the vet gave her some sort of anti-nausea shot. She then peed/pooped herself. The vet brought her to the back and she died shortly after. He thinks it might have been a stroke but I wasn't able to get a clear answer from him.

She has never had any seizures or strokes before and was in very good health.

I just want to know if this shot had anything to do with her death. It really came out of nowhere.

1 H NMR spectroscopic studies on the 1:1 adduct of the pentasaccharide Fondaparinux (FPX) and the substitution‐inert polynuclear platinum complex TriplatinNC show significant modulation of geometry around the glycosidic linkages of the FPX constituent monosaccharides. FPX is a valid model for the highly sulfated cell signalling molecule heparan sulfate (HS). The conformational ratio of the 1 C 4 : 2 S 0 forms of the FPX residue IdoA(2S) is altered from ≈ 35:65 (free FPX) to ≈ 75:25 in the adduct; the first demonstration of a small molecule affecting conformational changes on a HS oligosaccharide. Functional consequences of such binding are suggested to be inhibition of HS cleavage in MDA‐MB‐231 triple‐negative breast cancer (TNBC) cells. We further describe inhibition of metastasis by TriplatinNC in the TNBC 4T1 syngeneic tumour model. Our work provides insight into a novel approach for design of platinum drugs (and coordination compounds in general) with intrinsic anti‐metastatic potential.

https://ift.tt/3klCCdp