Cyclooxygenase Puns

So, I like to look at the Mechanism of Action of drugs, and I looked at aspirin, and I saw, "due to its irreversible inactivation of the cyclooxygenase," and I figured, if it's irreversible and that enzyme helps with the pain creation, why doesn't it permanently dull pain, because you can't deactivate it, it's irreversible.

Edit: Typo in the title, it's meant to be, "Why doesn't aspirin---"

Today we’re going to be focusing specifically on the pain relief properties of kavalactones. Ibuprofen, naproxen (Aleve), and aspirin are common drugs which fall into the class called “NSAIDs”. Non-steroidal anti-inflammatory drugs share a common quality. They inhibit the activity of one, the other, or both cyclooxygenase enzymes known as COX-1, and COX-2. Cyclooxygenase is an enzyme that is responsible for forming prostanoids that play a major role in pain and inflammation [1]. Most NSAIDs work by non-selectively blocking both COX-1 and COX-2 enzymes, thus reducing inflammation and pain. Kava was shown to inhibit both COX-1 and COX-2 enzymes substantially with all kavalactones. In fact, kava was shown to inhibit COX-1 enzymes by a greater or equal degree across all kavalactones in comparison to Ibuprofen, naproxen, and aspirin. Dihydromethysticin and yangonin were shown to be as good as the three pain relievers mentioned in regards to COX-2 inhibition [2], similar to drugs like Celebrex. If you’re noticing relief from aches and pains after drinking, or maybe seeing relief from arthritis this could lend evidence towards why.

https://i.imgur.com/redeUAW.jpg [2]

[1] Cyclooxygenase. (2020, December 02). Retrieved from https://en.wikipedia.org/wiki/Cyclooxygenase

[2] Wu D, Yu L, Nair MG, DeWitt DL, Ramsewak RS. Cyclooxygenase enzyme inhibitory compounds with antioxidant activities from Piper methysticum (kava kava) roots. Phytomedicine. 2002 Jan;9(1):41-7. doi: 10.1078/0944-7113-00068. PMID: 11924763.

This is rather interesting.

Ketogenic diets attenuate cyclooxygenase and lipoxygenase gene expression in multiple sclerosis. https://www.ncbi.nlm.nih.gov/m/pubmed/30292675/?i=2&from=multiple%20sclerosis

Hey guys I had a question about the COX inflammatory pathway. I was wondering if you increase the expression of COX-2, would this lead to an increase in COX-2 derived prostaglandins and a decrease in COX-1 derived products? I am asking this because I thought that if there's more COX-2 than COX-1, a larger amount of arachidonic acid will be acted upon by COX-2 and it's coupled-enzymes (mPGES1, mPGES2, PGIS).

Thanks in advance!

Activity‐based sensing of COX‐2: An isoform‐selective fluorogenic probe reports on enzymatic activity within live cells. A two‐step activation facilitates the release of the natural product and resorufin for confocal imaging and flow cytometry. CoxFluor indicates that COX‐2 activity can be regulated by oxygen without a change in the protein expression level.

Abstract

Cyclooxygenase‐2 (COX‐2) overexpression is prominent in inflammatory diseases, neurodegenerative disorders, and cancer. Directly monitoring COX‐2 activity within its native environment poses an exciting approach to account for and illuminate the effect of the local environments on protein activity. Herein, we report the development of CoxFluor, the first activity‐based sensing approach for monitoring COX‐2 within live cells with confocal microscopy and flow cytometry. CoxFluor strategically links a natural substrate with a dye precursor to engage both the cyclooxygenase and peroxidase activities of COX‐2. This catalyzes the release of resorufin and the natural product, as supported by molecular dynamics and ensemble docking. CoxFluor enabled the detection of oxygen‐dependent changes in COX‐2 activity that are independent of protein expression within live macrophage cells.

https://ift.tt/2SaDHuo

[Join the Battle for Net Neutrality] (https://www.battleforthenet.com)

I'll take some COX up my ass this once to stop laws allowing ISPs like COX Com to anal you everyday.

[Join the Battle for Net Neutrality] (https://www.battleforthenet.com)

I don't want to step on anybody's toes here, but the amount of non-dad jokes here in this subreddit really annoys me. First of all, dad jokes CAN be NSFW, it clearly says so in the sub rules. Secondly, it doesn't automatically make it a dad joke if it's from a conversation between you and your child. Most importantly, the jokes that your CHILDREN tell YOU are not dad jokes. The point of a dad joke is that it's so cheesy only a dad who's trying to be funny would make such a joke. That's it. They are stupid plays on words, lame puns and so on. There has to be a clever pun or wordplay for it to be considered a dad joke.

Again, to all the fellow dads, I apologise if I'm sounding too harsh. But I just needed to get it off my chest.

Do your worst!

I'm surprised it hasn't decade.

For context I'm a Refuse Driver (Garbage man) & today I was on food waste. After I'd tipped I was checking the wagon for any defects when I spotted a lone pea balanced on the lifts.

I said "hey look, an escaPEA"

No one near me but it didn't half make me laugh for a good hour or so!

Edit: I can't believe how much this has blown up. Thank you everyone I've had a blast reading through the replies 😂

It really does, I swear!

Hey guys I had a question about the COX inflammatory pathway. I was wondering if you increase the expression of COX-2, would this lead to an increase in COX-2 derived prostaglandins and a decrease in COX-1 derived products? I am asking this because I thought that if there's more COX-2 than COX-1, a larger amount of arachidonic acid will be acted upon by COX-2 and it's coupled-enzymes (mPGES1, mPGES2, PGIS).

Thanks in advance!