So I just made this story up to remember the cell cycle check points, I hope someone can get some use out of it:
The Capital letters correspond to the molecule Letters.
Its kind of a restaurant scene:
I want to Eat 2! (Checkpoint for G1/S, Cyclin E binds to Cyclin dependent kinase 2)
Dad says: we are a party of 4. (Cyclin D binds to CDK4)
I want to have Root Beer but mom says no so, so Dad buys her a Purse to persuade her and now the waiter can go to the kitchen door. (Here Cyclin D + CDK4 activated complex Phosphorylates protein RB which allows for the crossing of the checkpoint)
To further allow for the S-phase,
I want an Appetizer 2! (CDK 2 and cyclin A)
For the checkpoint between G2 and Mitosis, Can I have 1 Banana split for dessert? (get it, because its mitosis:). (Here CDK1 and cyclin B).
I hope this makes some sort of sense.
Some statements indicated that there were 3 more checkpoints founded in S phase, anaphase and telophase. So is it like 6 checkpoints? Am I getting the right information?
So I know Cyclins and CDKs regulate transition through the cell cycle.
But I'm a bit confused in terms of cell cycle checkpoints because in G1 (DNA damage checkpoint) p53 blocks entry in S Phase after its phosphorylated and stimulates p21. And in G2 checkpoint cdc25 is inactivated and blocks dephosphorylation and hence blocks entry into mitosis. And also because p21 is a negative regulator and thus halts the cell cycle.
So does this mean cdc25, p21 and cdks regulate these checkpoints or would we say cyclins, cdks and CKIs regulate it as cyclins are integral part of the cell cycle?
hey all, i’m using the thermo dycycle ruby stain with a bd accuri c6 machine. no matter what cell line I use, my data shows high (>50%) cell cycle arrest in my untreated controls. could anyone offer assistance as to why this happens? I use a protocol wherein after counting cells I add 1uL of dye, incubate in the dark at 37C for 15-20 min, and then read on the machine. I’ve tried using different batches of cells, different confluencies, no matter what I get the high G2 arrest
Journal of the American Chemical SocietyDOI: 10.1021/jacs.1c02016
Yu Yang, Jun Xu, Yang Sun, Liuting Mo, Bo Liu, Xiaoshu Pan, Zhuang Liu, and Weihong Tan
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