Acetylated distarch phosphate Puns

I tried a proper extraction once but it failed due to

A. me making my solution way too basic in the calcium morphenate conversion step (it was like ~11 when it should have been more around 8,5-9)

B. using Ca(OH)2 Powder instead of a saturated solution (stupid ik, dont have a lot of lab experience)

C. Not checking the temp properly and generally missing lots of equip that would have helped

Now I‘ll tackle this properly once I‘ve got some cash for a nice sep funnel, solvents, a hot plate, a glass filter aparatus etc.

But for the time being, could I just react powdered opium with Acetic Anhydride (or AcCl, could use both, anhydride seems smarted if I dont want to properly clean the product later, although I could just neutralize it with NaCO3) ? I dont see a problem unless I cant get it properly dry….

I understand that Disodium Acetyl Glucosamine Phosphate is a hyaluronic acid precursor. And that its usually achieved through an enzyme reaction of n-acetyl glucosamine. But since I cant do that at home where can I buy it raw? Any special considerations in formulating with it?

I don't get glycosylation. Why can asparagine and arginine be N-linked glycosylated but lysine cannot? Don't they both have N? Is it because lysine is NH3 whereas the others are NH2? Also, what in the heck is acetylation and which amino acids can and can't be acetylation and WHY? I'm lost y'all.

As far as phosphorylation to my understanding it's just the aa's with OH groups and sometimes His which also doesn't make sense but ok.

There was some debate on whether or not LFP batteries would ever be made in the 4680 cell format, and it seems that debate was laid to rest during the earnings call.

LFP batteries are less energy dense, I get it, but are the so much so that it’s worth losing the other benefits of the 4680 form factor, like structural pack, and tab-less design which would reduce internal resistance, plus all the economies of scale savings you’d have by not having other form factors to deal with.

https://youtu.be/eKOMVRGkwqs

tl;dr for the non-science people:

‘Yeast Nutrient’ product mixtures (specific product names, NOT yeast nutrients in general) contain both DAP and urea but are urea heavy. Unmetabolized urea after fermentation creates carcinogens and is banned in US pro winemaking but not homebrew. Reasonable alternatives exist without downsides, such as pure diammonium phosphate or organic nitrogen via yeast lysate. Turns out that LD Carlson’s mixture is 84% urea and 16% diammonium phosphate.

The posted image also shows post-separation (left) and pre-separation (right) mixtures. The DAP crystals are translucent and mostly cubic. Urea crystals are ball-shaped and opaque white. It is extremely easy to tell if your mixture has urea and if urea is the primary component just by looking at it. Source DAP from somewhere that doesn’t have urea if you use it or switch to another nitrogen source such as the Fermaid or Fermax series of products.

Abstract-

“Yeast nutrient” mixes sold by vendors like LD Carlson are mixes of diammonium phosphate (DAP) and urea. Both are primary nitrogen sources for yeast during fermentation, however, urea causes the formation of ethyl carbamate (EC). EC is a known carcinogen and disallowed in the US as an authorized supplement for winemaking by the Bureau of ATF. Homebrew supplies are not subject to this and there is no restriction

Some members of the community attempted to contact LD Carlson about how much urea is in there product but no answers were given. This experiment was designed to determine that via a simple solubility separation and gravimetric analysis. The findings were 84% urea and 16% diammonium phosphate.

Materials-

1. 1g of ‘Yeast nutrient’ mixtures
2. 30mL of 96% ethanol
3. Milligram scale
4. Filter paper

Procedure-

1. Weigh out about 1g of the nutrient mixture on a milligram scale.
2. Pipette 10mL of ethanol and stir the mixture for 10 minutes.
3. Decant or remove the wash ethanol.
4. Repeat #2 and #3 for a total of 3 washes.
5. Pour the remaining crystals onto tared filter paper and allow to dry.
6. Weigh the paper containing the crystals.

Observations-

0.759g of mixture was reduced to 0.121g of crystals at the end. This makes the mixture about 84% urea and 16% DAP, per the combination of experimental data and listed ingredients. No difficulties or complications conducting the experiment.

Discussion-

The urea-heavy composition makes it impossible to

I read that Calcium Nitrate precipitates while mixed together in high concentrations with Monopotassium Phosphate, and therefore you should store these nutrients in separate stock solution e.g. Part A and Part B.

However can anybody qualify what "high concentration" means?

I am planning a target nutrient solution with:

Calcium Nitrate, 380 ppm
Monopotassium Phosphate, 160 ppm

Should I worry about precipitation problem at all? Note that I will distribute this via an open, drain-to-waste system and the solution will be mixed via proportional pumps i.e. Dosatron.

Hi.

Rather reputable dude on youtube was saying that riboflavin 5 phosphate is just broken down to regular riboflavin and there is absolutely no use in taking R5P.

BUT, I was taking regular B2 since years, on and off, up to 300mg a day for various reasons. Like MTHFR stuff, PNPO Stuff (possible inability to convert pyridoxine to P5P) and possible MAOA warrior gene (MAOA deficiency), all based on my Dante Labs genetic testing. And also severe migraines.

The regular B2 did something, but the effect was very subtle. Also I mostly took it with regular B6 (not P5P). The idea was that B2 will help converting it to P5P already.

Now I tried one the same B6 pills (80mg) with one 80mg Warnke riboflavin 5 phosphate pill, and it just makes me so extremely relaxed to the point that I went back to bed dozing after taking them after waking up with no need to sleep anymore! Also it was kind of like a very happy high state.

This was reproducible 3 times on 3 different days in the past weeks. The combination makes a bit too tired and relaxed, BUT I'm the most agitated person due to ptsd, adhd and autism, among other things. Not even the strongest tranquilizers work so both me and doctors gave up on them.

Has anyone felt really different on riboflavin 5 phosphate compared to regular Riboflavin? It makes no sense scientifically but these seem like two completely different things to me AND I'm 100% sure about both containing the right thing and dosage so please help me out here :).

I want to try to make a mineral called phosphophyllite artificially, and it is made mostly of hidrated zinc phosphate, so I guess I can't use heat to melt it , and it's not water-soluble, so I can't do it by evaporation.

Should I bother memorizing these pathways as rigorously as Kreb's cycle and glycolysis? I hear that it is not necessary to know de novo nucleotide synthesis, keep hearing maybe. Nonetheless I hear maybe fatty acid and pentose phosphate synthesis is a must too. How in depth (IF AT ALL) should I learn Fatty acid synthesis and pentose phosphate synthesis? How of this is needed to be memorized, like Kreb's or just overview?

If they didn't they'd have no apatite!

Nature Chemistry, Published online: 22 March 2021; doi:10.1038/s41557-021-00655-9

The specificity of human and animal viruses that engage with O-acetylated sialic acids has now been probed using a collection of O-acetylated sialoglycans obtained by diversification of trisaccharide precursors with viral haemagglutinin–esterases. The results revealed host-specific patterns of receptor recognition and showed that human respiratory viruses uniquely employ 9-O-acetylated α2,8-linked disialosides as receptors.

https://ift.tt/2OP9wK0

So I don't have too much in my tank, 3 torches, one acan, some zoas, 2 favia ( small) and a bta and tube anemone. It's a 32 gallon and everything is basically frags except the anemones. Also have 2 clowns and a cleaner wrasse. I'm constantly at 0.0 even after feeding twice a day with flakes first then frozen rotifiers, mysis, brine all mixed together and broadcast feed. I put in much more than they eat up. I also broadcast red sea ab+ daily. I've removed my chemi pure and my small chaeto farm in the back of the AIO system for the past month so I just got filter floss and a teeny bit of activated carbon. I haven't done a water change in probably a month or more either ( just been dosing everything) my levels are all exactly where I wanna be except nitrate and phosphate. I dosed last night to 12ppm nitrate and about 0.15 phosphate and the next day I'm at 0 using Hannah checkers. I check every day and I'm always having to dose nitrate and phosphate daily it seems. I do have a small amount of algae but nothing crazy. In fact I do have a lot less since I stopped my weekly water changes but I still do get some. Someone said I should get another fish or 2 but it's not a big tank and I don't wanna over crowd it. Or should I feed even more? I am feeding a lot right now and I can imagine I'm getting some really fat bristle worms from it. Anyways if anyone has a suggestion or method to naturally raise the 2 parameters.

I just dosed them again and my current levels are

Nitrate 12ppm Phosphate 0.15 Nitrite 0 Ammonia 0 Magnesium 1440 Calcium 457 Kh 11 Ph 8.1 Temp 77.5 Salinity is 1.25 but I don't have an auto top off so when I get home from work it's 1.026 usually ( I get quite a bit of evaporation after going hoodless)

Everything else is pretty stable.

I have seen many people talk about daily maintenance from witch hazel to isopropyl rubbing alcohol. While I think all these do great, including hibacleanse, I have yet to see anyone discussing what works for me.

Everyone’s HS is different. What works for me, may not work for you. I think diet is immensely impactful in treatment of HS, and there are other subs that talk about dietary restrictions. I think this is the greatest thing you can do to treat your HS.

Personally, I have found Clindamycin Phosphate Solution 1.0% (Cleocin) Acne Treatment to work really effectively for me. The topical solution is rubbed against the problematic area, and I noticed over the years it has stopped flareups from occurring. You must get a prescription from your doctor, but I found this a very effective tool and daily treatment of HS.

I hope it works for you as well.

In Paget Disease of the Bone, the Alkaline Phosphatase is high but PO4 is normal. Does the alkaline phosphate use or produce PO4? If not, what does it to then?