Heard Alex Mack's perfuse sweating is affecting the Center-QB exchanges. Saw an infomercial for this product and wondered if it might help...
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๐Ÿ‘ค︎ u/johnnyradz
๐Ÿ“…︎ Aug 11 2021
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Perfuse perspiration problems?

Last week I got my Index headset replaced because the tracking on it died - pretty certain because of my sweating while playing this game as nowadays it's my main form of exercise and literally the only thing I play in VR.

I average around 45 mins per session and play every other day.

Today I was trying to train some more more long distance endurance and so I opted to skip the breaks while fighting on Outclassed. About 20 mins in, my left hand disappeared in game and I looked to see that my left controller was off and upon trying to turn it back on had seemed to have fully died.

It turns on now after drying it out (there was a pool of sweat on top) it's functional but the status light is not.

Anyone else mess their stuff up this way?

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๐Ÿ‘ค︎ u/T_O_Neill
๐Ÿ“…︎ Apr 27 2021
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Aniracetam perfuse sweating?

Has anyone had this side effect from aniracetam idk why but at the 1.5g mark I start sweating as if I was sprinting. so far itโ€™s my first experience with a nootropic and I do feel a boost in energy and mood but this one side effect for me kinda worries me.

I live in the desert and have gotten use to high temps where I barley sweat at regular 100 degree temps but even in an air conditioned room Iโ€™m dripping in sweat in less than 5 minutes after wiping off. I now stick to lower doses(.75 g every 4 hours) but Iโ€™m pretty sure itโ€™s lost itโ€™s โ€œkickโ€ after my tolerance built up. Just wanted to know if this is normal for anyone else?

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๐Ÿ‘ค︎ u/longpenisofthelaw
๐Ÿ“…︎ Aug 28 2020
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Induced V-tach with aortic waveform. Just to show how V-tach doesn't perfuse well.
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๐Ÿ‘ค︎ u/4077
๐Ÿ“…︎ Feb 11 2019
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Fluxion - Perfuse youtu.be/kMdXyh1ynQA
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๐Ÿ‘ค︎ u/Down2Earth77
๐Ÿ“…︎ Dec 27 2019
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Perfuse 73 - Popstar Beat (2019) open.spotify.com/track/4Eโ€ฆ
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๐Ÿ‘ค︎ u/TranceScythe
๐Ÿ“…︎ Mar 24 2019
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Do I have to perfuse for IHC?

Hi all, new here. I'm running some immediate early gene expression immunohistochemistry and I'm having troubles with perfusion. I'm doing this on convict cichlid fish and the perfusion process is very time-consuming (45+ min/fish) and I also have difficulty doing it because the fish are so small. The IHC I'm doing needs DAB, so I'm just wondering if there's any way that I could just extract a fresh brain and drop-perfuse it in 4% PFA and then use a H2O2 quenching step before my blocker so I don't have to properly perfuse. Any suggestions?

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๐Ÿ‘ค︎ u/neurostressnerd
๐Ÿ“…︎ Jan 09 2017
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It's completely necessary to perfuse the whole animal in order to fix an organ for immunohistochemisry?

I don't have acces to perfusion equipment. I was wondering if I can stick the pancreas into PFA 4%

EDIT: I meant Immunofluorescence on tissue slides.

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๐Ÿ‘ค︎ u/nemodot
๐Ÿ“…︎ Jul 04 2016
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I am absolutely in love with my diamondiferous lamproite. Perfused with beautiful gold mica and olivine inclusions. And I had to fight a herd of black widow spiders to get it.
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๐Ÿ‘ค︎ u/anarchophysicist
๐Ÿ“…︎ Dec 28 2021
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I was accepted into perfusion school but I donโ€™t know how I will afford housing if I canโ€™t work during school. Do loans cover housing?
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๐Ÿ‘ค︎ u/Logical-Ad-6139
๐Ÿ“…︎ Jan 13 2022
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Current Perfusion School Students

I will soon be attending perfusion school and was wondering if any current students would recommend getting an iPad with the pencil for notes/studying. I have a friend in med-school who swears by it and was wondering if any perfusion students use one or would recommend it.

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๐Ÿ‘ค︎ u/No-Bird-5
๐Ÿ“…︎ Jan 02 2022
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Student interested in cardiovascular perfusion - How do I find someone to interview?

Hi -

Because of COVID, many schools I'm applying to are allowing an interview over email with a perfusionist instead of formal shadowing. The problem is that I can't find names/emails of perfusionists I could ask.

I shadowed during a CABG some years back when I was thinking about medical school but I didn't take a name down because I was mostly there for the surgeon and anesthesiologist at that point.

Thanks!

P.S. I live in Chicago. :)

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๐Ÿ‘ค︎ u/CapybaraFan987
๐Ÿ“…︎ Jan 11 2022
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Metal arteries / perfuse the land with black blood / fueling us; rusting
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๐Ÿ‘ค︎ u/ShiftedClock
๐Ÿ“…︎ Aug 29 2017
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Perfusion programs differ in their interview process, may you shed light on the schools youโ€™ve interviewed with?

How many rounds?
How long are the segments?
Written, zoom, recorded?
Vibe among the persons conducting the interviews?

Please donโ€™t forget to name the school(s) youโ€™re referring to.

Thank you!

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๐Ÿ‘ค︎ u/AnonPaperEditor
๐Ÿ“…︎ Jan 10 2022
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Goths, whenever I last long enough to research perfusion
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๐Ÿ‘ค︎ u/petran1420
๐Ÿ“…︎ Nov 16 2021
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If perfusion has taught me one thing, ITโ€™S LABELS!! smh
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๐Ÿ‘ค︎ u/AnonPaperEditor
๐Ÿ“…︎ Jan 10 2022
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Sildenafil for the Treatment of Patients With COVID-19 and Perfusion Mismatch on Subtraction Computed Tomography Angiography: A Pilot, Placebo-Controlled Randomised Trial papers.ssrn.com/sol3/papeโ€ฆ
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๐Ÿ‘ค︎ u/luisvel
๐Ÿ“…︎ Dec 29 2021
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Current Status and Future Perspectives on Machine Perfusion: A Treatment Platform to Restore and Regenerate Injured Lungs Using Cell and Cytokine Adsorption Therapy

Pivoting to bone-marrow derived multipotent adult progenitor cells (MAPCs) in a different model of prolonged cold ischemia, this alternate cell therapy was considered within the setting of EVLP in human donor lungs [34]. After 4 h of EVLP, there was less overall inflammation in treated organs as assessed by histology and by a decreased number of neutrophils and eosinophils. Another study of MAPCs to treat warm ischemia instead found that porcine lungs treated at the start of 6 h of EVLP had fewer cytokines and neutrophils in BAL [35]. There were, however, no changes to physiologic measures including PVR, compliance or PaO2/FiO2ย ratio compared to controls.

The MSC effect on the increase or decrease of key cytokines varies across publications. Mordant et al. reported a rise in IL-8 within their control group that did not exist in the MSC group eventually labeled as the โ€œoptimal doseโ€ [31]. The findings of Nakajima et al. were more expansive with higher levels of HGF and IL-4, but lower levels of IL-12, IL-18 and IFN-y in the MSC-treated group [32]. In this study, however, there was no difference in IL-8 after EVLP as Mordant et al. had observed. A decrease in TNF-ฮฑ was only observed following transplantation, but not EVLP. Similarly, Lee et al.โ€™s work did not find decreased pro-inflammatory IL-8 or TNF-ฮฑ [25]. Only in the study of MAPCs in EVLP was a higher level of IL-10 found in cell therapy treatment compared to vehicle control [34]. Many of the studies were limited by relatively small sample sizes, leading to the implication that continued study of the interplay between cell treatment and cytokine levels could shed light on expected changes in concentrations. To this end, Nykรคnen et al. undertook an effort to gain greater control over cytokine manipulation, specifically by the genetic modification of MSCs to produce increased amounts of anti-inflammatory IL-10 [36]. These cells were then given at the start of 12 h of EVLP to 5 human lungs rejected for transplantation, with the finding that this translated to higher IL-10 in the EVLP perfusate and tissue. There were not, however, differences in PVR, oxygenation capacity and compliance, or other measures of pulmonary function.

https://www.mdpi.com/2073-4409/11/1/91/htm

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๐Ÿ‘ค︎ u/wisdom_man1
๐Ÿ“…︎ Dec 29 2021
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Is this Perfusion Index Variance / Floor Normal? (Left Hand VS Right Hand)

Background: Returned home from holidays sick, (suspected COVID). PI% is has a very low floor (0.x%/0.0x%), and is considerably variable on the left hand (But not the right). Ensured stable breathing (Despite struggling with the phone lol), normal seating position, and that my hands are dead still - - repeated twice with same results on each hand. I made a side by side 1m vid here. O2 Sat's are high as I just got off CPAP, and will drop to 94-97 (Which is Normal as day progresses).

Symptoms presenting as:

-Constantly runny nose/Blowing nose

-headache (sensitive to lights & sounds)

-mild confusion / "Mental Slowness," (Used to experiencing this with my history of Bacterial Pneumonia)

-fatigue (Could be related to MDD history, and procrastinating on going to sleep the night before - - but I did take 3MGs of Melatonin and got 12Hrs of sleep).

-No coughing (Only clearing throat), no phlegm expulsion. No chest pains, skin remains normal colour (Not blue). Cannot obtain COVID Test until Jan 1st.

Question: Should I be concerned about this low floor / variance? I'm not really that worried tbh (Which is why I'm posting on Reddit when my Doc said that I can call him anytime), as I know that clinical presentation matters more than a random test (And I just feel sick, like when I have Bacterial Pneumonia).

Demographics:

Location: Ontario, Canada

Age/Sex: 25M

Weight: 250LBs (Lost 15Lbs since Nov 8th).

Medical Conditions: Moderate OSA (On CPAP), MDD, provisional ADD, Clinician suspicions of ASD/ Non Specific Eating Disorder (Binge Eating).

Medications:

-Adderall XR 30MGs (11:14AM)

-Vortioxetine 20MGs (11:14AM)

-Perindopril / Amlodipine 7/5MGs (11:14AM)

-Ranitidine 150MGs (11:14AM & 4PM)

-Ozempic (Liraglutide) 0.25MGs: Dec 23rd (Dosage Escalation to .50MGs tomorrow)

Supplements:

-CoQ10 (Ubiquinone) 400MGs (11:14AM)

-PQQ 20MGs (11:14AM - - moreso just to take the last one, was just trying it out)

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๐Ÿ‘ค︎ u/WafflesCamus
๐Ÿ“…︎ Dec 30 2021
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TX Heart Institute School of Perfusion

Not sure if thereโ€™s a way to search in this group (Iโ€™m more of a Facebooker), but Iโ€™m wondering about peoplesโ€™ experience with the TX Heart Institute in Houston. Admittance experience, tuition assistance, real world experience after graduating, etc. Any info about this school would be so helpful. Thanks!

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๐Ÿ‘ค︎ u/ElishaDunaway
๐Ÿ“…︎ Jan 06 2022
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Ex-vivo Kidney Machine Perfusion: Therapeutic Potential

Cell Therapy

Another type of cells, potentially useful for kidney treatment duringย ex-vivoย machine perfusion, are multipotent adult progenitor cells (MAPC). Genetically MAPCs are similar to MSCs, reside in the bone marrow, and even have comparable function and mechanism of action. Particular growth and expansion characteristics lead to phenotypically different features of those two cells' populations (141). MAPC immunomodulatory capacity has already been demonstrated in a couple of Tx studies. Treatment with allogenic MAPC in a rat heterotopic heart transplant model allowed to withdraw pharmacological immunosuppressive therapy and successfully achieve long-term survival (142). MAPCs have also been successfully used in a human liver Tx case, which resulted in a pro-tolerogenic profile of the recipientโ€˜s leucocytes and reduced immunogenicity (143). Thompson et al., in their very recent study, usedย ex-vivoย NMP to deliver MAPC to non-transplantable human kidneys. MAPC treatment resulted in higher UO, lower NGAL concentration in perfusate, but not other kidney injury biomarkers kidney injury molecule-1 (KIM-1) and flavine mononucleotide (FMN). MAPC was also associated with the changes in cytokine profileโ€”decreased IL-1ฮฒ and increased IL-10 levels, as well as up-regulated indoleamine-2,3-dioxygenase activity, which is known for its role in pro-tolerant mechanisms and suppression of inflammatory processes (101). Although the results of machine perfusion cell-therapy studies are promising, several questions remain to be answered. The fate ofย ex-vivoย delivered MSC or MAPC is still not determined. It is known that their lifespan in the target organ is limited; however, there is evidence that once immunomodulatory processes have been promoted, these beneficial effects are maintained even after inactivation or death of MSC (144,ย 145). Therefore, it is necessary to investigate if supportive cell therapy would be beneficial after implantation and at which time points it would be the most efficient. Additionally, it is crucial to thoroughly check into possible immunogenicity of allogenic MSC and MAPC in the machine perfusion setting, as extraction and preparation of autologous cells in case of Tx is usually logistically demanding (146). The perfect duration and conditions of machine perfusion also need to be determined. As discussed previously, several hours ofย ex-vivoย cell therapy could be sufficient to promote immunomodulatory and anti-inflammatory processes; however

... keep reading on reddit โžก

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๐Ÿ‘ค︎ u/wisdom_man1
๐Ÿ“…︎ Dec 24 2021
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Cardiovascular Perfusion careers & courses.

So I am just wondering whether anyone who has completed their BSc or B Biomed degree with a biomedical sciences major considered pursuing a career as a Certified Cardiovascular Perfusionist (CCP)?

Perfusionists provide assistance to cardiothoracic surgeons & cardiac anaesthetists by stopping the heart & lungs via operation of a heart lung bypass machine, thereby enabling work to be done on either organ.

Usually involved in all aspects of cardiothoracic surgery (coronary artery bypass, mitral valve replacement, heart lung transplantation, artificial devices etc).

Outside operating suite environments, perfusionists assist & consult with ICU, nurses, administer ECLS (Extra Corporeal Life Support) & pursue research.

Most of the major metropolitan hospitals (RCH, RMH, St Vincent's, Austin, The Alfred) have a large cardiac surgery unit specialising in adult & paediatric (RCH) cases.

I have searched high & low; however other than the ANZCP website.

Should I simply contact the relevant cardiac surgery department register interest and schedule a suitable time to discuss options in potentially shadowing a perfusionist to inspect workplace environment, setting up the HLM circuit, formulation of priming solution etc.

Also would the Biomedical Engineering Systems major provide sufficient background knowledge & preparation for a career in cardiovascular perfusion?

Or should one just major in HSF, PHYS, BCMB, MIiM, PATH or PHRM?

Thank you!

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๐Ÿ‘ค︎ u/Horror_Tomorrow4660
๐Ÿ“…︎ Dec 31 2021
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Ventilation or perfusion issue?

Had a 50 yom today, called to his house for nausea. He was sitting upright kinda spaced out, but a/o x4. Found him to be hypoxic at 80% on room air, rr 20 non-labored, with clear lung sounds, and a distinctive cough that sounds like rhonchi. Hr 140 sinus tach, bp 102/80. 12 lead sinus tach. He was also experiencing general malaise and coughing up green sputum. 6lpm of o2 nasal cannula got him up to 97%. No other symptoms. Got him loaded into the rig and his sats dropped down to 90% but 15lpm of o2 non-rebreather fixed it back up to 96%. Half way into transport he became altered, gcs of 8 and his spo2 went to 80% despite o2 therapy. I place an end tidal cannula on him and his vitals were 138 hr sinus, 136/98, spo2 80% 15lpm, etco2 77 with a normal waveform, 33 rr, temp 99.8F. Upgraded it code 3 and gave my ringdown to the hospital since we were 5 minutes away. Do you guys think I should have placed a npa/opa and bagged?

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๐Ÿ‘ค︎ u/KhanSTiPate
๐Ÿ“…︎ Oct 22 2021
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Short of active processes (perfusion/cryonics), what is the second best and passive way to preserve as much of your brain tissues, with a view to resurrection?

If life sucks in my lifetime (political corruption, the bands I like being gone), maybe by the time future descendants of humanity resurrect me, maybe all things are sorted out by the time they do.

   

I'm placing a huge bet on progressively replacing my brain with computers and then shutting the component down for future activation, but that requires a huge investment to begin replacement. I read that plasticizing your brain answers my question, please correct me.

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๐Ÿ‘ค︎ u/WeTheSummerKid
๐Ÿ“…︎ Dec 27 2021
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Early Experience with Donation after Circulatory Death Heart Transplantation Using Normothermic Regional Perfusion in The United States perfusion.com/early-experโ€ฆ
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๐Ÿ‘ค︎ u/PerfusionCom
๐Ÿ“…︎ Jan 12 2022
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Injectable, Poreโ€Forming, Perfusable Doubleโ€Network Hydrogels Resilient to Extreme Biomechanical Stimulations onlinelibrary.wiley.com/dโ€ฆ
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๐Ÿ‘ค︎ u/joosth3
๐Ÿ“…︎ Dec 02 2021
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In this video we talk about ECMO and how RTs and Nurses can specialize without going to perfusion school youtu.be/wpeA0PEwhOU
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๐Ÿ‘ค︎ u/Level-You-9183
๐Ÿ“…︎ Dec 23 2021
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Can someone explain this concept to me? Iโ€™m having a hard time wrapping my head around the difference between cerebral perfusion pressure and cerebral blood flow
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๐Ÿ‘ค︎ u/iMazin77
๐Ÿ“…︎ Dec 07 2021
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Normothermic machine perfusion of the liver (NMP-L)

I'm looking forward to further Data in this area, I think it's potential is largely overlooked.

The Delivery of Multipotent Adult Progenitor Cells to Extended Criteria Human Donor Livers Using Normothermic Machine Perfusion

6/25/20

Background: Pre-clinical research with multi-potent adult progenitor cells (MAPCยฎ cells, Multistem, Athersys Inc., Cleveland, Ohio) suggests their potential as an anti-inflammatory and immunomodulatory therapy in organ transplantation. Normothermic machine perfusion of the liver (NMP-L) has been proposed as a way of introducing therapeutic agents into the donor organ. Delivery of cellular therapy to human donor livers using this technique has not yet been described in the literature. The primary objectives of this study were to develop a technique for delivering cellular therapy to human donor livers using NMP-L and demonstrate engraftment.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344318/

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๐Ÿ‘ค︎ u/Streeker74
๐Ÿ“…︎ Dec 15 2021
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What is the state-of-the-art method to calculate intravascular perfusion parameters in contrast-enhanced CT?

I want to create perfusion metric maps of the pulmonary vascular tree, but, in literature, it isnโ€˜t clear to me which is the โ€žstate-of-the-artโ€œ method for this. My target metrics are blood flow, blood volume and mean transit time.

I understand that, in the field of blood kinetics, the choice falls between two types of methods:

  • Deconvolution-based methods - a one-compartment model is assumed with an input and an output flow. The compartment represents a region in space enclosing perfused tissue and its respective capillary vasculature. The input represents blood being supplied by an artery, and the output, blood being drained to a vein. In practice, the compartment is an ROI of voxels in organ tissue, the input an ROI in a major artery, and the output an ROI in a major vein. Through Fickโ€˜s principle it was derived that the mentioned metrics can be calculated from the impulse response, which is obtained by the deconvolution of the concentration curve (average pixel time-intensity in ROI) of the arterial ROI (arterial input function) and the tissueโ€˜s ROI.
  • Non-deconvolution-based methods - metrics get calculated directly from the concentration curves, as derived by the conservation of mass principle applied to a bolus passing through an ROI of interest. Here, there is a bolus being supplied into and drained out of an interest volume. As above, the supply is an artery and the drain is a vein. Blood flow then gets calculated by the maximum slope method, and blood volume gets calculated by the ratio of the areas under curve of the arterial and venous concentration curves.

Repeating both methods for a number of ROIs yields metric maps. However, they always assume an arterial and venous input and output, as well as a compartment where the blood gets โ€ždeposited and withdrawnโ€œ.

Is it reasonable at all to use the same rationale for vessels? More precisely, the curve concentration of an initial segment of the vessel would serve as input, a second segment as compartment, and a third segment as output. And repeating this procedure would eventually lead to mapping the entire vessel tree. I havenโ€˜t seen this applied in literature - am I missing some important assumption thatโ€˜s unreasonable in the intravascular case?

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๐Ÿ‘ค︎ u/Tall-Ad-8658
๐Ÿ“…︎ Dec 26 2021
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Perfusion During COVID-19

For those of you that are experienced practicing Perfusionists, how did COVID-19 effect your job? Did you face cancelled surgeries, layoffs, lost wages? I have read that certain departments were hit hard due to the cancelation of elective surgeries. Is that something that was faced by perfusionists?

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๐Ÿ‘ค︎ u/Vodkashot7
๐Ÿ“…︎ Nov 28 2021
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Thoughts on PhD/MS perfusion program?

After accepting an interview for a program they emailed about starting a PhD/MS program where I would graduate with a PhD in whatever I want (medical related) and MS in pharmacology with perfusion certification. (5-6 year commitment)

I like the idea of a doctorate and think it would be useful as I eventually want to go into academia but other than that Iโ€™m not sure. Thoughts?

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๐Ÿ‘ค︎ u/swaggycat11
๐Ÿ“…︎ Nov 23 2021
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Any tips on preparing for interviews for perfusion programs?

Hello All,

I am thrilled to share that I have been invited to interview with the perfusion program at the U of A.

Does anyone have any pointers on how to prepare that they wouldn't mind sharing? I have never interviewed with any graduate program and any pointers would be greatly appreciated.

Thank you all in advance!

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๐Ÿ‘ค︎ u/oren-ishiiii
๐Ÿ“…︎ Nov 23 2021
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Research during perfusion school?

Hey yโ€™all, I was just wondering if there are research opportunities that one can be a part of while in perfusion school, or are there institutions that more opportunities over others? Is there a path you can take that will help you lean towards academia in perfusion over clinical?

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๐Ÿ‘ค︎ u/Jcar62
๐Ÿ“…︎ Nov 22 2021
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