Isoforms Puns

Does RNA-Seq protocols deal with pre and Mature RNA after all the eukaryotic post-processing? or is it mixed and dealt with afterwards with QC scripts?

DOI: https://doi.org/10.1016/j.ejmech.2021.113996

URL: https://www.sciencedirect.com/science/article/abs/pii/S022352342100845X?via%3Dihub

Looking for this article for my PhD. Would really appreciate it if someone with institutional access could help me out.

I'm looking at two sequences for HSP70 for the same species and from the same authors in ncbi. The only difference in the two titles are the terms "major" vs. "minor form". What exactly defines the protein as major as opposed to minor form? Is the major form more commonly expressed than the minor one?

Hello everyone,

I am a bioinformatician who is relatively new to long reads sequencing technologies, and I was interested in its application to transcriptomics. I would be very interested in discovering the differences in isoforms proportion between, for instance, control vs. patient.

I did a fair amount of research and was pleased that there are many bioinformatics tools available.

I am, however, not sure about which sequencing technology is best suited for that kind of application.

As far as I understood, on the one hand, we got SMRT from PacBio producing quality long reads with more or less 10kb length. And on the other hand, we got Oxford Nanopore which can produce very long reads up to 1 million bases but with a higher error rate.

Can anyone who had experience in transcriptomic with one or both technology give feedback on the pros and cons, or difficulties, you have experienced?

Thanks in advance for your advice.

Hello everyone, I am struggling to find a validation cohort to test the prognostic value of some isoforms that I derived from TCGA-BRCA project RNASeq data. If you have come across a dataset that contains isoform expression data or know any useful database/software etc. it would be very useful Thank you in advance!