Hello!
Does anyone have any experience running high percentage formamide solutions directly on agarose gels?
I am eluting biotinylated DNA from Dynabeads MyOne Streptavidin T1, which requires elution in 95% formamide + 10 mM EDTA (According to Invitrogen). My question is rather or not I can run these samples directly on the gel or if I need to do an EtOH precipitation first. I have seen examples and protocols of people running 60% formamide solutions, but nothing higher than that.
Thank you!
Twofold C‐H annulation of readily available formamides and alkynes without built‐in chelating groups was achieved, providing a series of chiral ferrocenes in 40‐98% yield and 93‐99% ee. Bulky chiral SPO ligand‐enabled Ni‐Al bimetallic catalysis proved to be critical to high reactivity and high selectivity.
https://ift.tt/2vbJtTp
Hello, i am wondering if anyone has any info on the proper disposal of a formamide-ethanol (85% formamide) solution? The msds seems to say that it shouldnt be reacted with acids or alkalis...
I am a biochemist/structural biologist graduate student and I'm currently working on an RNA project that is giving me some problems in regards to RNA degredation. I believe this degredation is due to contamination with RNAses. I know that formamide can protect RNA against RNAse degredation and I typically use formamide at a 50% concentration in all of my buffers to help with that. One step, however, involves extraction of RNA from a slice of acrylamide gel by placing the slice in an electric field and "electro-eluting" the RNA out of the slice into a dialysis bag of buffer. This buffer does not include formamide and is the most likely place where RNAses are degrading my RNA. I was wondering, if I included formamide in the electro-elution, if there would be any risk that the formamide might decompose into something nasty that would either be bad for me or for my RNA. The composition of the buffer is 20 mM Tris pH 7.5, 10 mM acetic acid, 500 uM EDTA. Is there any reason to believe under 100 V (couldn't tell you the number of amps) the formamide would degrade?
Hello women of science
I am a developmental biologist... and now also an experiential embryologist! i.e. I am going to have a baby. I do a lot of in situ hybridizations, which use formamide, which is a teratogen (boo!)
Does anyone know of in situ protocols that do not require formamide?
Thanks!
I don't want to step on anybody's toes here, but the amount of non-dad jokes here in this subreddit really annoys me. First of all, dad jokes CAN be NSFW, it clearly says so in the sub rules. Secondly, it doesn't automatically make it a dad joke if it's from a conversation between you and your child. Most importantly, the jokes that your CHILDREN tell YOU are not dad jokes. The point of a dad joke is that it's so cheesy only a dad who's trying to be funny would make such a joke. That's it. They are stupid plays on words, lame puns and so on. There has to be a clever pun or wordplay for it to be considered a dad joke.
Again, to all the fellow dads, I apologise if I'm sounding too harsh. But I just needed to get it off my chest.
Do your worst!
I'm surprised it hasn't decade.
For context I'm a Refuse Driver (Garbage man) & today I was on food waste. After I'd tipped I was checking the wagon for any defects when I spotted a lone pea balanced on the lifts.
I said "hey look, an escaPEA"
No one near me but it didn't half make me laugh for a good hour or so!
Edit: I can't believe how much this has blown up. Thank you everyone I've had a blast reading through the replies 😂
It really does, I swear!
Because she wanted to see the task manager.
Heard they've been doing some shady business.
but then I remembered it was ground this morning.
Edit: Thank you guys for the awards, they're much nicer than the cardboard sleeve I've been using and reassures me that my jokes aren't stale
Edit 2: I have already been made aware that Men In Black 3 has told a version of this joke before. If the joke is not new to you, please enjoy any of the single origin puns in the comments
They’re on standbi
A play on words.
Pilot on me!!
They were cooked in Greece.
Nothing, he was gladiator.
Or would that be too forward thinking?
Dad jokes are supposed to be jokes you can tell a kid and they will understand it and find it funny.
This sub is mostly just NSFW puns now.
If it needs a NSFW tag it's not a dad joke. There should just be a NSFW puns subreddit for that.
Edit* I'm not replying any longer and turning off notifications but to all those that say "no one cares", there sure are a lot of you arguing about it. Maybe I'm wrong but you people don't need to be rude about it. If you really don't care, don't comment.
What did 0 say to 8 ?
" Nice Belt "
So What did 3 say to 8 ?
" Hey, you two stop making out "
Two for Two: A highly enantioselective annulation of two C−H bonds with alkynes without built‐in chelating groups was achieved through Ni−Al bimetallic catalysis enabled by a bulky BINOL‐derived secondary phosphine oxide (SPO) ligand. This reaction uses readily available formamides as starting materials and provides concise access to a broad range of chiral ferrocenes in 40–98 % yield and 93–99 % ee.
Abstract
Twofold C−H annulation of readily available formamides and alkynes without built‐in chelating groups was achieved. Ni−Al bimetallic catalysis enabled by a bulky BINOL‐derived chiral secondary phosphine oxide (SPO) ligand proved to be critical for high reactivity and high selectivity. This reaction uses readily available formamides as starting materials and provides a concise synthetic pathway to a broad range of chiral ferrocenes in 40–98 % yield and 93–99 % ee.
https://ift.tt/2vbJtTp
