I wanted to simulate a peptide with a Fmoc group attached at N terminal in Gromacs with preferably gromos54a7 forcefield. I got the topology files for Fmoc from prodrg server but I don't know how to add them to the RTP file list. Looking at how it is arranged there I feel uncomfortable adding it. Any help is appreciated.
https://doi.org/10.1038/s41598-021-86126-0
https://pubmed.ncbi.nlm.nih.gov/33753839
Abstract
In patients with cardiovascular disorders, blood total ketone body (TKB) levels increase with worsening heart failure and are consumed as an alternative fuel to fatty acid and glucose. We investigated factors contributing to the increase in the blood TKB levels in patients with cardiovascular disorders. The study population consisted of 1030 consecutive patients who underwent cardiac catheterization. Covariance structure analyses were performed to clarify the direct contribution of hemodynamic parameters, including the left ventricular end-diastolic pressure (LVEDP), left ventricular end-systolic volume index (LVESVI), left ventricular end-diastolic volume index (LVEDVI), and B-type natriuretic peptide (BNP) levels, to TKB by excluding other confounding factors. These analyses showed that the TKB levels were significantly associated with the BNP level (Pโ=โ0.003) but not the LVEDP, LVESVI, or LVEDVI levels. This was clearly demonstrated on a two-dimensional contour line by Bayesian structure equation modeling. The TKB level was positively correlated with the BNP level, but not LVEDP, LVESVI or LVEDVI. These findings suggested that elevated blood TKB levels were more strongly stimulated by the increase in BNP than by hemodynamic deterioration. BNP might induce the elevation of TKB levels for use as an important alternative fuel in the failing heart.
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Open Access: True
Authors: Yusuke Kashiwagi - Tomohisa Nagoshi - Yasunori Inoue - Yoshiro Tanaka - Hirotake Takahashi - Yuhei Oi - Haruka Kimura - Kousuke Minai - Michihiro Yoshimura -
Additional links:
B-Type Natriuretic Peptides and Cardiac Troponins for Diagnosis and Risk-Stratification of Syncope
Jeanne du Fay de Lavallaz, Patrick Badertscher, Thomas Nestelberger, Tobias Zimmermann, รscar Mirรณ, Emilio Salgado, Michael Christ, Nicolas Geigy, Louise Cullen, Martin Than, F. Javier Martin-Sanchez, Salvatore Di Somma, W. Frank Peacock, Beata Morawiec, Joan Walter, Raphael Twerenbold, Christian Puelacher, Desiree Wussler, Jasper Boeddinghaus, Luca Koechlin, โฆ Show all Authors Originally published25 Feb 2019https://doi.org/10.1161/CIRCULATIONAHA.118.038358Circulation. 2019;0
Thanks in advance
Journal of the American Chemical SocietyDOI: 10.1021/jacs.0c05186
https://ift.tt/2V810Wk
http://stke.sciencemag.org/content/10/489/eaam6870
When fat is more important than muscle
>Although natriuretic peptides were originally identified as modifiers of blood pressure, they also exert metabolic effects, and obese individuals have decreased circulating natriuretic peptide concentrations. Wu et al. sought to determine whether these metabolic effects were exerted by signaling in skeletal muscle or adipose tissue. Mice with an adipose tissueโspecific deficiency in the natriuretic peptide clearance receptor, which acts to limit natriuretic peptide signaling, were protected from the detrimental metabolic effects of diet-induced obesity, such as insulin resistance, inflammation, and hepatic steatosis. In contrast, mice with a muscle-specific deficiency in the clearance receptor gained weight and developed insulin resistance on a high-fat diet, similar to wild-type mice. These findings suggest that enhancing natriuretic peptide signaling in adipose tissue could be a way to counteract obesity.
Abstract
>In addition to controlling blood pressure, cardiac natriuretic peptides (NPs) can stimulate lipolysis in adipocytes and promote the โbrowningโ of white adipose tissue. NPs may also increase the oxidative capacity of skeletal muscle. To unravel the contribution of NP-stimulated metabolism in adipose tissue compared to that in muscle in vivo, we generated mice with tissue-specific deletion of the NP clearance receptor, NPRC, in adipose tissue (NprcAKO) or in skeletal muscle (NprcMKO). We showed that, similar to Nprc null mice, NprcAKO mice, but not NprcMKO mice, were resistant to obesity induced by a high-fat diet. NprcAKO mice exhibited increased energy expenditure, improved insulin sensitivity, and increased glucose uptake into brown fat. These mice were also protected from diet-induced hepatic steatosis and visceral fat inflammation. These findings support the conclusion that NPRC in adipose tissue is a critical regulator of energy metabolism and suggest that inhibiting this receptor may be an important avenue to explore for combating metabolic disease.
We report here an operationally very simple method to greatly facilitate chemical protein synthesis by fully convergent and oneโpot native chemical ligations utilizing the Fluorenylmethyloxycarbonyl (Fmoc) moiety as an Nโmasking group of the Nโterminal cysteine of the middle peptide thioester segment(s). The Fmoc group is stable to harsh oxidative conditions frequently used to generate peptide thioester from hydrazide or oโaminoanilide. The ready availability of the FmocโCys(Trt)โOH, routinely used in Fmoc chemistry solid phase peptide synthesis, where the Fmoc group is preโinstalled on cysteine residue, minimizes additional steps required for the temporary protection of the Nโterminal cysteinyl peptides. The Fmoc group is readily removed after ligation reaction by short exposure (
https://ift.tt/358jmKk
Hey all! I had a ton of blood tests done to me today at my doctor's office. I will discuss them with my doctors tomorrow. However, one of the results was for B Type Natriuetic Peptide with a value of 498 pg/mL but a standard range listed on the form was 99 pg/mL
What exactly is this? My level is nearly 5 times higher than the standard range. I plan on asking about this tomorrow too.
I am 27 year old male, that is 5'6" and weigh about 155 pounds. I'm Caucasian and take a beta blocker to help regulate my third heartbeat.
Can i stack them?
