Or vice versa. My idea here is obviously to speed up the breakdown of plastic waste in landfills. I’ve always been fascinated with fungus and understand that both species use enzymes they produce to break down their respective substrates. Since Pestalotiopis produces that enzyme but is slow (though functions anaerobically) could we use CRISPR or similar tech to teach that gene to a prolific light tolerant fungi like Trichoderma or Pleurotus?
I was inoculating my second set of bags since I’m always messing with my first ones I want to take these serious. I’m injecting on front of the bag close to the viewing window. As I’m going down I poke the the tip of the needle enough to create a tiny hole smaller then the syringe, I assumed it should be fine if I covered it with microspore tape to avoid contamination. Will they be fine? Time will tell.
Quick question you guys. Is it possible to do ziplock tek without microspore tape?
So ive just aquired a pressure cooker ($80 from target, what a deal. Digital and everything. Isnt big but thats ok). And i just finished making some holes in some mason jar lids with silicone over the top and bottem to create a self sealing inoculation port. Now im about to drill my air exchange hole in the lid also but i was wondering what i should use to cover it. Apparently masking tape works fine? Is that really breathable tho? Would cotton fiber work? Or do i need to order some microspore to add to the list of magic makers hahaha. Also a few more quick questions for whoever can be fucked to read them -im using jars that are slightly thinner at the top, i have BRF and realised i cant do BRF cakes because i would need to cut the myc out of the jars (not ideal at all). Would grain to bulk spawn work instead? Would it break up easier? I heard rye grain works great? I am going to pick some up tomorrow to try it out but thought if someone has a better sugestion that would be much appreciated
- to make a spore syringe, do i just scrape some spores from a spore print into some distilled water and then suck up into a sterile syringe? Is that it? The spores have been sitting in a paper bag in my draw for ages, so my guess is this is all gonna be contam galore but thats ok, im just gonna use what i have and then order some prints online later if it doesnt work out. Ps, love all of you people, enjoy your weekend :) mushlove
the tek i was using said not to fully tighten the lids, however im using micropore tape w/ self healing injection ports, do i still need to cover the jars with tin foil? and should i tighten the lids fully since there is a hole in the lid covered w/ micropore tape?
Currently gathering all the supplies needed while doing extensive research to be ready. I came across different methods on inoculation and storage with microspore tape if it’s needed during certain stages. Sooo I guess my question is there a general or updated consensus on when to use tape if at all for inoculated holes? Any words of wisdom or general storage advice is greatly appreciated also! Thanks :)
P.s for context, trying to be as clean as possible and will be clearing out a carpeted room and storing in closet. Going to clean like to cover up a murder scene lol
The funeral director was asking us what we think Mum should wear in her casket.
Mum always loved to wear sarongs (fabric wraps that go around the torso and drape downward a bit like a long skirt would), so my uncle suggested that she wear a sarong in there.
The funeral director looked a bit confused, as did some of our family members, to which my uncle added:
"What's sarong with that?"
I started laughing like an idiot. He was proud of it too. The funeral director was rather shocked. We assured her, and our more proper relatives, that Mum would've absolutely loved the joke (which is very true).
His delivery was perfect. I'll never forget the risk he took. We sometimes recall the moment as a way help cushion the blows of the grieving process.
--Edit-- I appreciate the condolences. I'm doing well and the worst is behind me and my family. But thanks :)
--Edit-- Massive thanks for all the awards and kind words. And the puns! Love 'em.
I would have a daughter
Capital of Ireland
It's Dublin everyday
http://m.imgur.com/ImM3RWz
But Bill kept the Windows
True story; it even happened last night. My 5-year-old son walks up behind me and out of the blue says, "hey."
I turn to him and say, "yeah, kiddo? What's up?"
He responds, "it's dead grass."
I'm really confused and trying to figure out what's wrong and what he wants from me. "What? There's dead grass? What's wrong with that?"
.
.
.
He says, totally straight-faced, "hay is dead grass," and runs off.
You officially hit rock bottom
And then you will all be sorry.
Now it’s syncing.
He replied, "Well, stop going to those places then!"
She said how do you know he was headed to work?
I will find you. You have my Word.
“thank you for your cervix.”
