Or is it just one of those things that I should memorize because it will come up in M1 and M2 anyways?
Hi so Iβm kinda confused with other names of DNA Polymerase iii and DNA Polymerase i, cause like in my lecture, the lecturer calls it DNA Polymerase iii and DNA Polymerase i, but in the assignment it is called DNA Polymerase alpha, delta, epsilon. Sorry if its a dumb question cause I canβt think of a good search on googleπ
Primase adds RNA primers in the beginning of each okazaki fragment and then DNA pol. III adds DNA bases until the RNA primer of the next fragment. After they're joined together by DNA ligase and the primers need to be removed, does DNA pol. I just remove uracil and add adenine IF there happens to be any in the RNA primers? Or does it remove the whole RNA primer? If it does remove it all, 1) why? Apart from uracil, the rest of the sequence set by the primase should be correct, right? 2) how would it know exactly which 10 nucleotides were set by the primase? Bonus question: is exonuclease an enzyme that's part of DNA pol. I ? Or a seperate enzyme?
Passage link (question 4):https://jackwestin.com/daily/mcat-practice-passages/biological-biochemical-practice-passages/signaling-intermediates
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I found this online: "Reverse transcriptase contains three enzymatic activities: (1) RNA-dependent DNA polymerase, (2) RNase H, and (3) DNA-dependent DNA polymerase. First, RNA-dependent DNA polymerase synthesizes a DNA strand complementary to the RNA template. Then RNase H removes the RNA strand from the RNAβDNA hybrid double helix. Then the DNA-dependent DNA polymerase completes double-stranded DNA synthesis."
So if RT has activity (3), why is DNA polymerase needed in cDNA plasmid formation?
There is a word for this that I forgot and cannot seem to find. Polymerase activity is _______, which means that it doesn't easily fall off the DNA strand and continues for a long time along the DNA strand. It's something along the lines of "persistent" or "robust"
PROCESSIVITY that's the word!
Like between the Kaplan book and the internet... this shit got me heated. This isn't my job dude
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Hello everyone,
As the question says once the primer is cut out by RNAse H, how is DNA polymerase able to replace those bases if there is free 3'OH. Unless I am mistaken, there would be still be one primer at the very ends which would not be able to be replaced.
Thank You,
DNA-Protein Cross-links (DPCs) between DNA epigenetic mark 5-formylC and lysine residues of histone proteins spontaneously form in human cells. Such conjugates are likely to influence chromatin structure and mediate DNA replication, transcription, and repair, but are challenging to study due to their reversible nature. Here we report the construction of site specific, hydrolytically stable DPCs between 5fdC in DNA and K4 of histone H3 and an investigation of their effects on DNA replication. Our approach employs oxime ligation, allowing for site-specific conjugation of histones to DNA under physiological conditions. Primer extension experiments revealed that histone H3-DNA crosslinks blocked DNA synthesis by hPol Ξ· polymerase, but were bypassed following proteolytic processing.
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